FastQCFastQC Report
Fri 10 Feb 2017
ERR1630382.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630382.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2268214
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT69680.3072020541271679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT57140.25191626539647494No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53010.2337081069070202No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29340.12935287411152563No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG28990.1278098098327583No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT23630.10417888259220691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18250.022.9095881
CGTATCG502.703226E-422.210
TCGCCAT5850.016.76068313
TATACTG3150.016.4444455
ATCTCGC5700.015.90350811
AGGTCGC6050.015.90082610
TATCCCG700.00259353715.8571425
CGAATTA2500.015.5415
GTCGCCA6400.015.320312512
TTGGCCG6200.015.21774131
TTGCGCG1101.4530282E-515.13636418
CGGCCTT6050.014.98347124
GGTCGCC6500.014.80000111
CGCACGT750.004106045714.79999924
TCTCGCC6200.014.62096712
GGCCGCA6600.014.57575933
GAGGTCG6750.014.5259269
GTATAGA2450.014.3469381
TACGTTA1956.730261E-1014.2307719
CGGGAGT1702.4399924E-814.14705854
Produced by FastQC (version 0.11.5)