FastQCFastQC Report
Fri 10 Feb 2017
ERR1630381.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630381.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195052
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT121970.555658818105448No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT94090.4286458817376536No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT88090.4013116773543406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49260.22441381798699983No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGA555.145144E-420.1818186
CGAACGA2000.018.516
GACGGAC2700.017.129637
GTTCTAA1352.2222594E-816.4444431
TACTCCG1302.5918962E-715.6538465
CGGACCA2850.015.5789479
ACGGACC3100.015.51612958
ATCTCGC5250.015.50476211
ATACCGC3850.015.37662327
GGTATCA45050.015.0299651
TCACGTA750.00410598914.825
AACGAAC2550.014.50980414
GAGTCGT1152.2115444E-514.4782616
TACCGCA4100.014.43902428
CGAGCCG4000.014.33750115
TCGCCAT6200.014.32258113
TAGCACT6200.014.3225814
AAGACGG3250.014.2307695
ACGAACG2600.014.23076915
TCTCGCC5650.014.07964612
Produced by FastQC (version 0.11.5)