##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630381.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2195052
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85558018671084	33.0	31.0	34.0	30.0	34.0
2	32.058088373305054	33.0	31.0	34.0	30.0	34.0
3	32.18066314602114	34.0	31.0	34.0	30.0	34.0
4	35.819222961460596	37.0	35.0	37.0	35.0	37.0
5	35.753550713149394	37.0	35.0	37.0	35.0	37.0
6	35.81534970469948	37.0	35.0	37.0	35.0	37.0
7	35.79517706186459	37.0	35.0	37.0	35.0	37.0
8	35.80089811084202	37.0	35.0	37.0	35.0	37.0
9	37.53913028028493	39.0	37.0	39.0	35.0	39.0
10	37.44007431259032	39.0	37.0	39.0	34.0	39.0
11	37.50738342417401	39.0	37.0	39.0	35.0	39.0
12	37.43838915889008	39.0	37.0	39.0	34.0	39.0
13	37.44370247265213	39.0	37.0	39.0	34.0	39.0
14	38.66925886038235	40.0	38.0	41.0	34.0	41.0
15	38.68095835542848	40.0	38.0	41.0	34.0	41.0
16	38.639031330465066	40.0	38.0	41.0	34.0	41.0
17	38.631981839154605	40.0	38.0	41.0	34.0	41.0
18	38.595587712728445	40.0	38.0	41.0	34.0	41.0
19	38.64043357514993	40.0	38.0	41.0	34.0	41.0
20	38.631714875091795	40.0	38.0	41.0	34.0	41.0
21	38.57236958395519	40.0	38.0	41.0	34.0	41.0
22	38.53550895377422	40.0	38.0	41.0	34.0	41.0
23	38.47006676834991	40.0	38.0	41.0	34.0	41.0
24	38.4358274883693	40.0	38.0	41.0	34.0	41.0
25	38.41471728232406	40.0	38.0	41.0	34.0	41.0
26	38.25593380020155	40.0	38.0	41.0	34.0	41.0
27	38.12948030388346	40.0	38.0	41.0	33.0	41.0
28	38.061090580086486	40.0	38.0	41.0	33.0	41.0
29	37.99573495297606	40.0	37.0	41.0	33.0	41.0
30	37.91299067174718	40.0	37.0	41.0	33.0	41.0
31	37.83980470622108	40.0	37.0	41.0	33.0	41.0
32	37.73187742249387	40.0	37.0	41.0	33.0	41.0
33	37.629956374609804	40.0	37.0	41.0	33.0	41.0
34	37.55913891789352	40.0	37.0	41.0	32.0	41.0
35	37.427642716436786	40.0	36.0	41.0	32.0	41.0
36	37.32758631686174	40.0	36.0	41.0	31.0	41.0
37	37.26182022111549	40.0	36.0	41.0	31.0	41.0
38	37.13788056046052	40.0	36.0	41.0	31.0	41.0
39	37.04648773696477	39.0	36.0	41.0	31.0	41.0
40	36.93132782275773	39.0	36.0	41.0	31.0	41.0
41	36.80223612014658	39.0	35.0	41.0	30.0	41.0
42	36.74121569785135	39.0	35.0	41.0	30.0	41.0
43	35.55631666129094	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	0.0
10	2.0
11	6.0
12	6.0
13	4.0
14	3.0
15	6.0
16	14.0
17	45.0
18	94.0
19	186.0
20	354.0
21	682.0
22	1280.0
23	2278.0
24	3522.0
25	5668.0
26	8600.0
27	12918.0
28	18107.0
29	25428.0
30	33835.0
31	44405.0
32	57110.0
33	74302.0
34	97149.0
35	129544.0
36	180673.0
37	278622.0
38	494277.0
39	725929.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.418220616185856	19.970916406536155	12.785528543287358	24.82533443399063
2	17.700036263377818	22.717457262971447	35.059625011161465	24.52288146248927
3	19.374575180906874	24.54730001840503	31.086871746090754	24.99125305459734
4	14.249184073999158	16.186541366673772	35.6671277035806	33.89714685574647
5	13.62528085895004	37.940741267177266	34.39699833990265	14.036979533970037
6	32.481326182705466	36.345653770389035	16.682292720172462	14.490727326733033
7	27.339352325138538	32.23723173756248	22.172641012604714	18.250774924694266
8	26.731166277609823	33.882978626474454	21.104693647348675	18.281161448567048
9	26.50251565794341	14.57204658477339	20.75522584430802	38.170211912975184
10	16.349270996769096	27.78034415585599	33.62981833687767	22.240566510497246
11	33.88434533669361	22.962326177238626	22.40001603606657	20.753312450001186
12	20.633770862831497	26.97708300304503	30.31700387963474	22.072142254488732
13	29.098809504285093	20.570446622676823	26.253182156960293	24.077561716077795
14	22.192184968738783	21.95619967089618	26.413269480631897	29.438345879733145
15	24.37618789896549	28.567432571073486	23.853967924222296	23.202411605738725
16	23.663812975729048	27.587410229917104	25.046969274531993	23.701807519821855
17	22.359880312630406	27.567775159768427	26.744150024691898	23.32819450290927
18	21.909549295415324	26.103709615990873	28.518504345227356	23.468236743366443
19	22.83968671357216	26.505431306410966	28.74551491263077	21.909367067386103
20	23.622447213095636	25.734105615721177	28.383154476522655	22.260292694660535
21	23.667229751276963	26.474999225530876	27.104004825398214	22.753766197793947
22	23.38883087963292	26.228034688927643	27.54850454567819	22.83462988576125
23	22.635454649821508	26.454954142316446	27.69510699518736	23.214484212674687
24	23.266601429032203	26.606613419636528	27.7259946461405	22.400790505190766
25	23.20765066157886	26.164938233809494	27.653832346568556	22.97357875804309
26	22.970799780597453	26.625610691682933	27.573059772615867	22.83052975510375
27	23.115534392807096	26.286484329300624	27.289877415204742	23.308103862687535
28	22.37974316781561	26.710984523373476	28.003755719682268	22.90551658912864
29	22.345438741314556	26.99867702450785	27.975783717196677	22.680100516980918
30	22.14927026785698	27.808862842429242	27.754604446728372	22.287262442985405
31	22.995172779505907	27.092706687586443	27.54208100764811	22.37003952525954
32	22.041619059594034	27.07393720057657	27.656702438028802	23.227741301800595
33	22.151639232236867	26.63353761095409	28.179970223940025	23.034852932869015
34	22.533361396449834	26.38333852683217	28.086897258014844	22.996402818703157
35	22.731488821221546	26.56292424963053	28.201837587446676	22.503749341701244
36	22.205852070930437	27.101954760069464	27.929634468796184	22.762558700203915
37	22.963692887457793	26.114233284678452	27.75123322818776	23.170840599675998
38	22.264073926266896	26.503609026118742	28.596771283778246	22.635545763836117
39	22.31559890152944	26.069678531533647	28.59034774574816	23.024374821188744
40	21.883900700302316	25.78672395915905	29.396433433012064	22.932941907526562
41	21.323412839422485	26.18603112819195	29.570142301868017	22.92041373051755
42	21.96881896191981	25.566774727887996	29.368461430526477	23.09594487966572
43	21.235260030286298	25.314343350408098	29.556702984712892	23.89369363459271
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	588.0
1	573.5
2	559.0
3	1404.0
4	2249.0
5	2249.0
6	3099.5
7	3950.0
8	3724.0
9	3498.0
10	4807.0
11	6116.0
12	6116.0
13	10217.5
14	14319.0
15	21888.5
16	29458.0
17	27672.0
18	25886.0
19	25886.0
20	28295.5
21	30705.0
22	30413.0
23	30121.0
24	34150.5
25	38180.0
26	38180.0
27	42757.0
28	47334.0
29	58756.5
30	70179.0
31	75003.5
32	79828.0
33	79828.0
34	85605.5
35	91383.0
36	98203.5
37	105024.0
38	114515.5
39	124007.0
40	124007.0
41	132221.5
42	140436.0
43	134758.5
44	129081.0
45	135110.0
46	141139.0
47	141139.0
48	143107.5
49	145076.0
50	147606.5
51	150137.0
52	156730.0
53	163323.0
54	163323.0
55	150572.0
56	137821.0
57	130447.0
58	123073.0
59	111853.0
60	100633.0
61	100633.0
62	91041.5
63	81450.0
64	70904.5
65	60359.0
66	51032.5
67	41706.0
68	41706.0
69	35008.0
70	28310.0
71	24604.0
72	20898.0
73	16542.0
74	12186.0
75	12186.0
76	9169.5
77	6153.0
78	5152.5
79	4152.0
80	3191.0
81	2230.0
82	2230.0
83	1732.0
84	1234.0
85	1064.0
86	894.0
87	817.0
88	740.0
89	740.0
90	626.0
91	512.0
92	303.0
93	94.0
94	57.5
95	21.0
96	21.0
97	13.5
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2195052.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.92883361009167
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.46080227986062	39.959171442253236
2	13.015696213698122	13.257484535738637
3	4.008266894237095	6.124090731643198
4	1.5711923686347815	3.2007597884658643
5	0.7917214125586041	2.0160724042871947
6	0.44506369389334344	1.3599944887312119
7	0.2824167673140127	1.0068209585864731
8	0.2053374181699831	0.8366076163123909
9	0.13559149963778647	0.6214965231596069
>10	0.8568488153780464	8.347826801403878
>50	0.09780471612415133	3.5475213930738407
>100	0.11520675955353171	12.354568784105009
>500	0.010918736526613876	3.7633753061673185
>1k	0.0028639308922265884	2.217887254109385
>5k	1.7899568076416177E-4	0.830380196785071
>10k+	8.949784038208089E-5	0.5559417751777095
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12197	0.555658818105448	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9409	0.4286458817376536	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8809	0.4013116773543406	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4926	0.22441381798699983	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.555700730552169E-5	0.0	0.0	0.0	0.0
6	4.555700730552169E-5	0.0	0.0	0.0	0.0
7	4.555700730552169E-5	0.0	0.0	4.555700730552169E-5	0.0
8	4.555700730552169E-5	0.0	0.0	4.555700730552169E-5	0.0
9	4.555700730552169E-5	0.0	0.0	9.111401461104338E-5	0.0
10	4.555700730552169E-5	0.0	0.0	9.111401461104338E-5	0.0
11	4.555700730552169E-5	0.0	0.0	1.8222802922208676E-4	0.0
12	4.555700730552169E-5	0.0	0.0	3.1889905113865184E-4	0.0
13	4.555700730552169E-5	0.0	0.0	3.1889905113865184E-4	0.0
14	4.555700730552169E-5	0.0	0.0	4.100130657496952E-4	0.0
15	4.555700730552169E-5	0.0	0.0	5.011270803607387E-4	0.0
16	4.555700730552169E-5	0.0	0.0	8.655831388049122E-4	0.0
17	4.555700730552169E-5	0.0	0.0	0.0016400522629987809	0.0
18	4.555700730552169E-5	0.0	0.0	0.0017767232849153459	0.0
19	4.555700730552169E-5	0.0	0.0	0.002323407372581606	0.0
20	4.555700730552169E-5	0.0	0.0	0.0031434335040809967	0.0
21	4.555700730552169E-5	0.0	0.0	0.004100130657496952	0.0
22	4.555700730552169E-5	0.0	0.0	0.005603511898579168	0.0
23	4.555700730552169E-5	0.0	0.0	0.008291375329604948	0.0
24	4.555700730552169E-5	0.0	0.0	0.01211816394326877	0.0
25	4.555700730552169E-5	0.0	0.0	0.013894887228184116	0.0
26	4.555700730552169E-5	0.0	0.0	0.01694720671765407	0.0
27	4.555700730552169E-5	0.0	0.0	0.034851110588724095	0.0
28	4.555700730552169E-5	0.0	0.0	0.0718434005208077	0.0
29	4.555700730552169E-5	0.0	0.0	0.12519065607557361	0.0
30	4.555700730552169E-5	0.0	0.0	0.20564433097712492	0.0
31	4.555700730552169E-5	0.0	0.0	0.4089196975743627	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGA	55	5.145144E-4	20.181818	6
CGAACGA	200	0.0	18.5	16
GACGGAC	270	0.0	17.12963	7
GTTCTAA	135	2.2222594E-8	16.444443	1
TACTCCG	130	2.5918962E-7	15.653846	5
CGGACCA	285	0.0	15.578947	9
ACGGACC	310	0.0	15.5161295	8
ATCTCGC	525	0.0	15.504762	11
ATACCGC	385	0.0	15.376623	27
GGTATCA	4505	0.0	15.029965	1
TCACGTA	75	0.004105989	14.8	25
AACGAAC	255	0.0	14.509804	14
GAGTCGT	115	2.2115444E-5	14.478261	6
TACCGCA	410	0.0	14.439024	28
CGAGCCG	400	0.0	14.337501	15
TCGCCAT	620	0.0	14.322581	13
TAGCACT	620	0.0	14.322581	4
AAGACGG	325	0.0	14.230769	5
ACGAACG	260	0.0	14.230769	15
TCTCGCC	565	0.0	14.079646	12
>>END_MODULE