FastQCFastQC Report
Fri 10 Feb 2017
ERR1630380.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630380.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404472
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG52450.21813520806231054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41660.17326049128457308No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40350.1678123097295373No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT35720.14855652301212074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33990.14136159622569944No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG31800.13225356751918926No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC30300.1260151916928124No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA28720.11944410248902879No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC24100.10022990494378808No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13050.025.3754791
GTACCGT450.003827005420.5555556
GACGGAC2850.016.8771937
TAACGCC2300.016.0869564
TATCCGG700.00259359715.85714310
ACGGACC3200.015.6093758
GTATCAA21500.015.4883722
CGGACCA3000.015.4166679
TCTATAC3400.015.2352943
TCGTTTA2201.8189894E-1215.13636430
AATAACG2450.015.102042
GCTTAGG12250.014.951021
GCATCGC2600.014.94230714
AAGACGG3600.014.9027785
CGGCCTT8850.014.84180724
GCGTTAT2251.8189894E-1214.81
AACGCCG2251.8189894E-1214.85
GCGAAAG3400.014.69117618
TCGCCAT8350.014.62275413
AGGTCGC8650.014.32947910
Produced by FastQC (version 0.11.5)