FastQCFastQC Report
Fri 10 Feb 2017
ERR1630379.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630379.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1748986
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25040.14316867030382177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT22270.12733092203139418No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG21140.12087003555202842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18600.10634733497009125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAA400.001931587623.12500233
GGTATCA11000.021.8636381
TCGAGTA450.00382659420.5555572
TCTAGCG2000.019.42528
CTAGCGG2150.018.06976729
TACGCAT1054.800804E-717.6190528
AAGACGG2550.017.4117645
CGCAATA2250.017.26666636
TTCCTCG3850.016.81818219
CGGCCTT3750.016.2824
TATACTG2850.016.228075
TAAACCG700.00259321815.8571435
GTATACG700.00259321815.8571431
CCCGATT700.00259321815.85714314
GACGGAC2700.015.0740737
ATCTCGC3950.014.98734311
GTATCAA15950.014.84639552
ATCCGTC750.004105544614.830
CTAGACT1405.9995E-714.5357154
TAGACTA908.277056E-414.3888895
Produced by FastQC (version 0.11.5)