##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630379.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1748986
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896721300227675	33.0	31.0	34.0	30.0	34.0
2	32.113376550755696	33.0	31.0	34.0	30.0	34.0
3	32.23566054845493	34.0	31.0	34.0	30.0	34.0
4	35.86433281913063	37.0	35.0	37.0	35.0	37.0
5	35.790132110834506	37.0	35.0	37.0	35.0	37.0
6	35.8430387664624	37.0	35.0	37.0	35.0	37.0
7	35.825116953480475	37.0	35.0	37.0	35.0	37.0
8	35.82179903098138	37.0	35.0	37.0	35.0	37.0
9	37.57892573182404	39.0	37.0	39.0	35.0	39.0
10	37.46984252589786	39.0	37.0	39.0	35.0	39.0
11	37.53908264560151	39.0	37.0	39.0	35.0	39.0
12	37.46360176696669	39.0	37.0	39.0	35.0	39.0
13	37.46943886343287	39.0	37.0	39.0	35.0	39.0
14	38.73078629560214	40.0	38.0	41.0	35.0	41.0
15	38.74617406886047	40.0	38.0	41.0	35.0	41.0
16	38.704309811513646	40.0	38.0	41.0	35.0	41.0
17	38.67988480182231	40.0	38.0	41.0	34.0	41.0
18	38.62432346513923	40.0	38.0	41.0	34.0	41.0
19	38.66235693138767	40.0	38.0	41.0	34.0	41.0
20	38.6389548000956	40.0	38.0	41.0	34.0	41.0
21	38.585147622679656	40.0	38.0	41.0	34.0	41.0
22	38.55728805147668	40.0	38.0	41.0	34.0	41.0
23	38.50239109975723	40.0	38.0	41.0	34.0	41.0
24	38.467446852061705	40.0	38.0	41.0	34.0	41.0
25	38.44366278517953	40.0	38.0	41.0	34.0	41.0
26	38.30166107676105	40.0	38.0	41.0	34.0	41.0
27	38.184473174742394	40.0	38.0	41.0	33.0	41.0
28	38.1266974121005	40.0	38.0	41.0	33.0	41.0
29	38.080503217292765	40.0	38.0	41.0	33.0	41.0
30	38.02507910297738	40.0	38.0	41.0	33.0	41.0
31	37.97800382621702	40.0	37.0	41.0	33.0	41.0
32	37.89650345971894	40.0	37.0	41.0	33.0	41.0
33	37.815563417888995	40.0	37.0	41.0	33.0	41.0
34	37.763597307239735	40.0	37.0	41.0	33.0	41.0
35	37.64385020806341	40.0	37.0	41.0	32.0	41.0
36	37.568726107584624	40.0	37.0	41.0	32.0	41.0
37	37.52651650727908	40.0	37.0	41.0	32.0	41.0
38	37.4174647481455	40.0	37.0	41.0	31.0	41.0
39	37.34633095976754	40.0	37.0	41.0	31.0	41.0
40	37.257962042006056	40.0	36.0	41.0	31.0	41.0
41	37.14958381599395	40.0	36.0	41.0	31.0	41.0
42	37.1143308179711	39.0	36.0	41.0	31.0	41.0
43	35.90911934114967	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	0.0
12	1.0
13	1.0
14	1.0
15	5.0
16	12.0
17	29.0
18	74.0
19	141.0
20	247.0
21	549.0
22	895.0
23	1546.0
24	2487.0
25	4142.0
26	6192.0
27	9257.0
28	13093.0
29	18559.0
30	24915.0
31	33305.0
32	43351.0
33	56295.0
34	74761.0
35	99321.0
36	139425.0
37	211792.0
38	404169.0
39	604416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.40954473048955	19.20621434362539	12.234460424497394	26.14978050138766
2	18.294085830303956	21.225212780433917	34.92515091601648	25.555550473245642
3	19.430115506928015	23.32420042241619	30.173311850409323	27.07237222024648
4	14.514295711915363	15.654556411543602	35.11040111241599	34.720746764125046
5	14.311320959687498	36.83116960341592	34.61542859691272	14.242080839983853
6	34.203589965843065	35.575870818863045	15.986234309479894	14.234304905813996
7	28.99897426280142	30.825518328906004	21.61223703334389	18.563270374948683
8	26.722340830629864	34.09289725589571	20.367801686234195	18.81696022724024
9	26.616851135457914	14.486736886401609	19.620511542116404	39.275900436024074
10	16.514940657043567	26.83126108499439	33.581000648375685	23.072797609586353
11	35.395137525400436	21.98702562513365	21.816755537208415	20.8010813122575
12	21.135846713467117	25.644058900414297	29.691832867730216	23.52826151838837
13	29.70824237586808	19.85481873496986	25.47241658881203	24.96452230035003
14	22.731171089991573	20.994450498746133	25.021240879000743	31.253137532261547
15	25.057661982428677	27.958771539623527	22.359698705421312	24.62386777252648
16	25.003459147185854	26.769682547487516	23.766971262205644	24.459887043120982
17	23.612824802485555	26.751672111726453	25.29076847956473	24.344734606223266
18	23.38629354380195	25.51284001129798	26.70753225011521	24.393334194784863
19	24.314088277436184	25.694602472518362	26.481744279256663	23.509564970788787
20	24.715120647049204	25.057490454469043	26.317477669918453	23.909911228563292
21	24.5973381147705	25.793116697332053	25.326160415234884	24.28338477266256
22	24.53581675325017	25.362524342676267	25.78362548356591	24.318033420507653
23	23.853078297939494	25.696317752114656	25.8866566113165	24.563947338629355
24	24.469263904914047	25.91158534144927	25.82187621856321	23.797274535073466
25	24.62666939586709	25.296543254205577	25.79471762495526	24.282069724972068
26	24.28904519533032	25.97545091841787	25.709982812898446	24.02552107335336
27	24.759203332673906	25.250059177146074	25.520101361588942	24.47063612859108
28	23.968287910823758	25.48528118578422	26.2442352311568	24.30219567223523
29	23.681092930417968	25.93045341700848	26.164932137821573	24.223521514751976
30	23.547301121907207	26.680545184466887	25.98728634763229	23.78486734599362
31	24.406255967743597	26.016617628728877	25.60415006180724	23.97297634172029
32	23.626661391228975	25.806381526210043	25.872305438694195	24.694651643866788
33	23.64335677929955	25.608952844676857	26.245092870954938	24.502597505068653
34	24.361773050213095	25.27384438754798	26.162759450332935	24.201623111905985
35	24.476353727245385	25.439997804442115	26.365333970654998	23.7183144976575
36	23.797903470925437	26.129254322218703	25.978938653597	24.093903553258862
37	24.83256012340865	25.02798764541283	25.949893252433125	24.1895589787454
38	24.090987577945164	25.492771240021362	26.455386149460313	23.960855032573157
39	24.1895589787454	25.28853861608955	26.35229784572318	24.169604559441872
40	23.755821944829748	25.004602666916714	27.06288100648033	24.17669438177321
41	23.223627862086946	25.48568141769002	27.31245418774078	23.97823653248225
42	24.0651440320277	24.943367185329098	26.990095975611013	24.00139280703219
43	23.47748924233813	24.63804741718916	27.173859596360405	24.7106037441123
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	112.5
2	119.0
3	292.5
4	466.0
5	466.0
6	643.5
7	821.0
8	756.5
9	692.0
10	1006.5
11	1321.0
12	1321.0
13	2362.5
14	3404.0
15	5203.5
16	7003.0
17	7609.5
18	8216.0
19	8216.0
20	10232.5
21	12249.0
22	14268.5
23	16288.0
24	19598.5
25	22909.0
26	22909.0
27	26966.0
28	31023.0
29	38833.0
30	46643.0
31	51248.0
32	55853.0
33	55853.0
34	61711.5
35	67570.0
36	73784.5
37	79999.0
38	88525.5
39	97052.0
40	97052.0
41	103748.0
42	110444.0
43	107374.5
44	104305.0
45	110357.0
46	116409.0
47	116409.0
48	120271.0
49	124133.0
50	126633.0
51	129133.0
52	134602.0
53	140071.0
54	140071.0
55	130040.0
56	120009.0
57	113699.5
58	107390.0
59	98530.5
60	89671.0
61	89671.0
62	82791.5
63	75912.0
64	66806.5
65	57701.0
66	49864.0
67	42027.0
68	42027.0
69	35511.0
70	28995.0
71	25263.0
72	21531.0
73	17167.0
74	12803.0
75	12803.0
76	9832.0
77	6861.0
78	5651.5
79	4442.0
80	3360.0
81	2278.0
82	2278.0
83	1701.0
84	1124.0
85	977.0
86	830.0
87	749.5
88	669.0
89	669.0
90	543.0
91	417.0
92	243.0
93	69.0
94	46.0
95	23.0
96	23.0
97	13.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1748986.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.49474677100071
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.39503527785388	49.09765928800881
2	9.044539301613993	10.400269936133203
3	2.254881130453478	3.889314587823916
4	0.9489053338025091	2.182282875065087
5	0.493790590599055	1.4195182482197777
6	0.32602253462083797	1.1246749841798935
7	0.2251737553712426	0.9062415631181397
8	0.16247001303236802	0.7472937805741748
9	0.1274791925298144	0.6596445503765993
>10	0.8024803577076384	8.904726413873437
>50	0.1050900539928548	4.247769528637179
>100	0.10456360130778118	12.076754957860388
>500	0.008073723190373253	3.121120871542504
>1k	0.0014951339241431952	1.2227284145868837
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2504	0.14316867030382177	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2227	0.12733092203139418	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2114	0.12087003555202842	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1860	0.10634733497009125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	1.1435197308611962E-4	0.0	0.0	0.0	0.0
12	1.715279596291794E-4	0.0	0.0	5.717598654305981E-5	0.0
13	1.715279596291794E-4	0.0	0.0	5.717598654305981E-5	0.0
14	1.715279596291794E-4	0.0	0.0	5.717598654305981E-5	0.0
15	1.715279596291794E-4	0.0	0.0	1.715279596291794E-4	0.0
16	1.715279596291794E-4	0.0	0.0	5.145838788875383E-4	0.0
17	1.715279596291794E-4	0.0	0.0	6.861118385167176E-4	0.0
18	1.715279596291794E-4	0.0	0.0	6.861118385167176E-4	0.0
19	2.2870394617223924E-4	0.0	0.0	8.576397981458971E-4	0.0
20	2.2870394617223924E-4	0.0	0.0	9.719917712320167E-4	0.0
21	2.8587993271529904E-4	0.0	0.0	0.0010863437443181362	0.0
22	2.8587993271529904E-4	0.0	0.0	0.0016009276232056746	0.0
23	3.430559192583588E-4	0.0	0.0	0.0019439835424640334	0.0
24	3.430559192583588E-4	0.0	0.0	0.003259031232954409	0.0
25	3.430559192583588E-4	0.0	0.0	0.0038879670849280667	0.0
26	3.430559192583588E-4	0.0	0.0	0.004859958856160084	0.0
27	3.430559192583588E-4	0.0	0.0	0.00903380587380345	0.0
28	3.430559192583588E-4	0.0	0.0	0.02052617916895847	0.0
29	4.0023190580141865E-4	5.717598654305981E-5	0.0	0.03796485506459171	0.0
30	4.0023190580141865E-4	5.717598654305981E-5	0.0	0.06266488125119354	0.0
31	4.0023190580141865E-4	5.717598654305981E-5	0.0	0.14860038902541245	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	40	0.0019315876	23.125002	33
GGTATCA	1100	0.0	21.863638	1
TCGAGTA	45	0.003826594	20.555557	2
TCTAGCG	200	0.0	19.425	28
CTAGCGG	215	0.0	18.069767	29
TACGCAT	105	4.800804E-7	17.61905	28
AAGACGG	255	0.0	17.411764	5
CGCAATA	225	0.0	17.266666	36
TTCCTCG	385	0.0	16.818182	19
CGGCCTT	375	0.0	16.28	24
TATACTG	285	0.0	16.22807	5
TAAACCG	70	0.002593218	15.857143	5
GTATACG	70	0.002593218	15.857143	1
CCCGATT	70	0.002593218	15.857143	14
GACGGAC	270	0.0	15.074073	7
ATCTCGC	395	0.0	14.987343	11
GTATCAA	1595	0.0	14.8463955	2
ATCCGTC	75	0.0041055446	14.8	30
CTAGACT	140	5.9995E-7	14.535715	4
TAGACTA	90	8.277056E-4	14.388889	5
>>END_MODULE