FastQCFastQC Report
Fri 10 Feb 2017
ERR1630378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1741671
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG55960.3213006359984176No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG40230.2309850712333156No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36550.20985593720053902No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT34650.19894687343361633No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA31910.18321485515921204No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31130.17873639740226482No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT29990.17219095914211122No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC28000.16076515024938692No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC24250.1392341033409869No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT23430.1345259810836834No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC21690.1245355753181858No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC21490.12338725281640449No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT21170.12154993681355435No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC20480.11758822418240873No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG20140.11563607592938047No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC19500.1119614439236802No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC18960.10886097316887058No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA18410.10570308628897192No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA18210.10455476378719059No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA18170.1043250992868343No Hit
TCTCCAGACTCACTGGTTTTCCCAGAGGCAAATGGCTCCCAGG18070.10375093803594365No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA17870.1026026155341623No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT450.003826587520.5555578
CGAACGA801.6174503E-518.516
TTAGACT609.2394237E-418.54
CGCACTA500.007036302218.529
GTATAGG1501.2732926E-1118.4999981
GCTTAGG13200.017.659091
CGTTATT1603.45608E-1117.343752
TCTTATA9450.017.22751437
CCTGTCG650.001580376117.07692312
CTTAGGA14400.016.9583342
ATTACAC1201.04208084E-716.9583323
TAGGACA14800.016.8754
GGTATCA18650.016.863271
ATGTGCT8250.016.81818237
GATGTGC8500.016.75882336
TTAGGAC15500.016.3516123
TCGCCAT7950.016.28930913
CGGCCTT8300.016.27108424
ACTTTTG9600.016.187530
AAGACCG1151.2430173E-616.0869565
Produced by FastQC (version 0.11.5)