##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630378.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1741671
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89075893208304	33.0	31.0	34.0	30.0	34.0
2	32.0877714562624	33.0	31.0	34.0	30.0	34.0
3	32.22354336726052	34.0	31.0	34.0	30.0	34.0
4	35.864373351798356	37.0	35.0	37.0	35.0	37.0
5	35.7777565338115	37.0	35.0	37.0	35.0	37.0
6	35.84468536250532	37.0	35.0	37.0	35.0	37.0
7	35.820973651166035	37.0	35.0	37.0	35.0	37.0
8	35.800341166615276	37.0	35.0	37.0	35.0	37.0
9	37.521876979062064	39.0	37.0	39.0	35.0	39.0
10	37.443678513335755	39.0	37.0	39.0	34.0	39.0
11	37.52137860709629	39.0	37.0	39.0	35.0	39.0
12	37.40707860439773	39.0	37.0	39.0	34.0	39.0
13	37.42627166669251	39.0	37.0	39.0	34.0	39.0
14	38.68102529122894	40.0	38.0	41.0	35.0	41.0
15	38.69313090704272	40.0	38.0	41.0	35.0	41.0
16	38.66129825897084	40.0	38.0	41.0	34.0	41.0
17	38.62976130394317	40.0	38.0	41.0	34.0	41.0
18	38.5926486690081	40.0	38.0	41.0	34.0	41.0
19	38.62481145979924	40.0	38.0	41.0	34.0	41.0
20	38.57732717602808	40.0	38.0	41.0	34.0	41.0
21	38.540692817415	40.0	38.0	41.0	34.0	41.0
22	38.52814969072804	40.0	38.0	41.0	34.0	41.0
23	38.482199565819265	40.0	38.0	41.0	34.0	41.0
24	38.474917478674215	40.0	38.0	41.0	34.0	41.0
25	38.440510291553345	40.0	38.0	41.0	34.0	41.0
26	38.29134032776569	40.0	38.0	41.0	34.0	41.0
27	38.198842376085956	40.0	38.0	41.0	34.0	41.0
28	38.13638626353657	40.0	38.0	41.0	33.0	41.0
29	38.08530026623857	40.0	38.0	41.0	33.0	41.0
30	38.036301919248814	40.0	38.0	41.0	33.0	41.0
31	37.93150084028499	40.0	37.0	41.0	33.0	41.0
32	37.836266436083505	40.0	37.0	41.0	33.0	41.0
33	37.79398922069667	40.0	37.0	41.0	33.0	41.0
34	37.720377155042485	40.0	37.0	41.0	33.0	41.0
35	37.6316233088798	40.0	37.0	41.0	32.0	41.0
36	37.546645147102986	40.0	37.0	41.0	32.0	41.0
37	37.500031865949424	40.0	37.0	41.0	32.0	41.0
38	37.393464092816615	40.0	37.0	41.0	32.0	41.0
39	37.31031520878513	40.0	36.0	41.0	31.0	41.0
40	37.231914638298505	39.0	36.0	41.0	31.0	41.0
41	37.09744377669491	39.0	36.0	41.0	31.0	41.0
42	37.07221283468577	39.0	36.0	41.0	31.0	41.0
43	35.89188084316728	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	2.0
12	0.0
13	5.0
14	1.0
15	1.0
16	11.0
17	22.0
18	76.0
19	138.0
20	260.0
21	418.0
22	902.0
23	1556.0
24	2457.0
25	3989.0
26	6122.0
27	8905.0
28	12796.0
29	18262.0
30	24864.0
31	32920.0
32	43207.0
33	56720.0
34	75723.0
35	102913.0
36	144074.0
37	218890.0
38	403875.0
39	582558.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.523760227964985	18.12805059049614	12.677308171290674	26.670881010248205
2	18.029869016593832	20.890684865281674	35.22094586176149	25.858500256362998
3	19.721233229467565	22.357437196806977	30.21006837686337	27.71126119686209
4	15.175139277165433	15.338315904668562	33.15941989043855	36.32712492772745
5	14.35592600439463	37.00543902952968	34.19026900028766	14.448365965788026
6	35.603452087104856	33.85702581027071	15.610583169840917	14.928938932783517
7	29.97747565412756	30.440479286845797	20.42584391656059	19.156201142466056
8	26.76045016538715	35.05593191825551	19.637405686837525	18.546212229519814
9	26.614153878660208	14.88323569721262	18.413293899938623	40.089316524188554
10	17.017163402272875	27.55279269161627	32.4360341304414	22.994009775669458
11	35.63147115614832	21.898337860594797	20.8270103825579	21.643180600698983
12	21.280138441760815	25.47949641465007	28.470187538289377	24.770177605299736
13	30.044020943105785	19.301980684067196	25.451132848856066	25.202865523970946
14	23.27075549859876	20.27392084957492	23.92168210873351	32.53364154309281
15	25.635438610391976	27.42848678079844	21.844079622385628	25.09199498642396
16	25.631706562261186	26.463953295427206	23.078583727925654	24.825756414385953
17	24.56566136773248	26.84714851427164	24.33140357736909	24.25578654062679
18	23.276726775608022	25.539668513743415	26.116011577387464	25.067593133261102
19	25.28020504446592	25.512970015576997	25.804069769778565	23.402755170178523
20	25.009086101795347	24.584551272886785	26.381675988174575	24.024686637143294
21	24.328934683990262	25.619993672743014	24.860378337814662	25.19069330545206
22	24.718847589470112	24.847172629044177	24.873756294960415	25.560223486525295
23	23.859270780761694	25.96741864565696	25.269181148448816	24.90412942513253
24	25.287956221352943	25.7445292480612	25.119784390967066	23.847730139618793
25	25.256894097679755	25.00736361804267	25.272339035328717	24.463403248948854
26	25.322118815780936	25.515438908955822	24.87582327546362	24.286618999799618
27	25.362941680719263	24.64713484923387	24.656378845373208	25.33354462467366
28	24.18924125164856	25.154119233770327	25.70031883174262	24.95632068283849
29	24.217317736817115	25.713581956638194	25.2771619898362	24.791938316708496
30	23.82757707971253	26.808162965336162	25.15589913364809	24.20836082130322
31	24.912454763270446	25.3779847054926	25.346233588318345	24.36332694291861
32	23.669108574466705	25.653639522045207	25.607075044597977	25.070176858890115
33	24.134006939312876	25.185238773568603	25.462788322249153	25.21796596486937
34	24.55343173308851	25.0980810956834	25.61769702773945	24.730790143488637
35	24.9583876633417	25.181506725437814	25.524510656719894	24.335594954500593
36	24.232762674466073	25.68447198121804	25.605754473720925	24.477010870594963
37	25.455840971113375	24.44962337892748	25.19138229895313	24.903153351006015
38	24.522427025540413	24.997545460652443	26.2434179589601	24.236609554847043
39	24.66608217051326	24.754732667650778	26.030404134879664	24.548781026956295
40	24.052590873936584	24.045758355050985	26.984602717734862	24.917048053277572
41	23.383750432774043	24.847919038670334	27.20249691244787	24.56583361610775
42	24.380436948195154	23.796916868914966	26.685177625395383	25.137468557494497
43	23.587807341340586	24.15525090559583	27.017559573535987	25.239382179527592
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	55.0
1	76.5
2	98.0
3	255.5
4	413.0
5	413.0
6	582.0
7	751.0
8	777.0
9	803.0
10	1191.5
11	1580.0
12	1580.0
13	2728.0
14	3876.0
15	6598.0
16	9320.0
17	8674.5
18	8029.0
19	8029.0
20	8966.0
21	9903.0
22	10360.0
23	10817.0
24	12397.0
25	13977.0
26	13977.0
27	16492.5
28	19008.0
29	28019.0
30	37030.0
31	40218.0
32	43406.0
33	43406.0
34	48538.5
35	53671.0
36	58007.5
37	62344.0
38	76983.0
39	91622.0
40	91622.0
41	100723.5
42	109825.0
43	104227.0
44	98629.0
45	110098.5
46	121568.0
47	121568.0
48	131216.5
49	140865.0
50	148449.0
51	156033.0
52	166126.5
53	176220.0
54	176220.0
55	151452.0
56	126684.0
57	117468.5
58	108253.0
59	96105.5
60	83958.0
61	83958.0
62	78758.5
63	73559.0
64	64775.0
65	55991.0
66	48466.0
67	40941.0
68	40941.0
69	34912.5
70	28884.0
71	25417.0
72	21950.0
73	17917.0
74	13884.0
75	13884.0
76	10936.0
77	7988.0
78	6608.0
79	5228.0
80	3778.0
81	2328.0
82	2328.0
83	1707.0
84	1086.0
85	825.5
86	565.0
87	435.0
88	305.0
89	305.0
90	241.5
91	178.0
92	103.5
93	29.0
94	21.0
95	13.0
96	13.0
97	7.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1741671.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.85276579894884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4220659188055	36.071520877238086
2	10.989582893281186	9.858263754809512
3	3.3701380238315233	4.534800344791426
4	1.5204758740665587	2.7279019312983754
5	0.8435272501287641	1.8917265097528397
6	0.5236529780789851	1.4092370631419286
7	0.37546620225857297	1.1788488344727173
8	0.25630450908719915	0.9196772895442159
9	0.19578676688910412	0.7903420201629341
>10	1.2139812619511983	10.265225350400899
>50	0.12543581280077498	3.9816822594314947
>100	0.1338636589746295	13.138377628383083
>500	0.02036766002371205	6.117769731704647
>1k	0.009223091331492248	6.793104217950223
>5k	1.2809849071517012E-4	0.32152218691766565
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5596	0.3213006359984176	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	4023	0.2309850712333156	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3655	0.20985593720053902	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	3465	0.19894687343361633	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3191	0.18321485515921204	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3113	0.17873639740226482	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2999	0.17219095914211122	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2800	0.16076515024938692	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2425	0.1392341033409869	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2343	0.1345259810836834	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	2169	0.1245355753181858	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2149	0.12338725281640449	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2117	0.12154993681355435	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2048	0.11758822418240873	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	2014	0.11563607592938047	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1950	0.1119614439236802	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1896	0.10886097316887058	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	1841	0.10570308628897192	No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA	1821	0.10455476378719059	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1817	0.1043250992868343	No Hit
TCTCCAGACTCACTGGTTTTCCCAGAGGCAAATGGCTCCCAGG	1807	0.10375093803594365	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1787	0.1026026155341623	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	2.2966450035626705E-4	0.0
12	0.0	0.0	0.0	2.2966450035626705E-4	0.0
13	0.0	0.0	0.0	2.2966450035626705E-4	0.0
14	0.0	0.0	0.0	2.870806254453338E-4	0.0
15	0.0	0.0	0.0	4.019128756234673E-4	0.0
16	0.0	0.0	0.0	7.464096261578679E-4	0.0
17	0.0	0.0	0.0	0.0014928192523157358	0.0
18	0.0	0.0	0.0	0.001722483752672003	0.0
19	0.0	0.0	0.0	0.0020669805032064036	0.0
20	0.0	0.0	0.0	0.002411477253740804	0.0
21	0.0	0.0	0.0	0.003387551380254939	0.0
22	0.0	0.0	0.0	0.005167451258016009	0.0
23	0.0	0.0	0.0	0.00746409626157868	0.0
24	0.0	0.0	0.0	0.010047821890586684	0.0
25	0.0	0.0	0.0	0.011310976642546152	0.0
26	0.0	0.0	0.0	0.013205708770485356	0.0
27	0.0	0.0	0.0	0.01825832777832323	0.0
28	0.0	0.0	0.0	0.03381809767746032	0.0
29	0.0	0.0	0.0	0.06160750222056864	0.0
30	0.0	0.0	0.0	0.09823899002739324	0.0
31	0.0	0.0	0.0	0.22484154584878543	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCCGT	45	0.0038265875	20.555557	8
CGAACGA	80	1.6174503E-5	18.5	16
TTAGACT	60	9.2394237E-4	18.5	4
CGCACTA	50	0.0070363022	18.5	29
GTATAGG	150	1.2732926E-11	18.499998	1
GCTTAGG	1320	0.0	17.65909	1
CGTTATT	160	3.45608E-11	17.34375	2
TCTTATA	945	0.0	17.227514	37
CCTGTCG	65	0.0015803761	17.076923	12
CTTAGGA	1440	0.0	16.958334	2
ATTACAC	120	1.04208084E-7	16.958332	3
TAGGACA	1480	0.0	16.875	4
GGTATCA	1865	0.0	16.86327	1
ATGTGCT	825	0.0	16.818182	37
GATGTGC	850	0.0	16.758823	36
TTAGGAC	1550	0.0	16.351612	3
TCGCCAT	795	0.0	16.289309	13
CGGCCTT	830	0.0	16.271084	24
ACTTTTG	960	0.0	16.1875	30
AAGACCG	115	1.2430173E-6	16.086956	5
>>END_MODULE