FastQCFastQC Report
Fri 10 Feb 2017
ERR1630377.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630377.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321952
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33731.0476717026140543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT28860.8964069178014114No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18830.5848697942550443No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12770.39664297783520525No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT6380.19816618626379087No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA5520.17145412980817015No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5150.1599617334261008No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4780.1484693370440314No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT4510.140082993738197No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4430.13759815127720904No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATAC4180.1298330185866216No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG4140.12859059735612763No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3920.12175728058841069No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3860.11989364874266971No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGG3620.1124391213597058No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGG3560.11057548951396481No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCG3520.10933306828347082No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC3500.10871185766822385No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGG3430.10653762051485936No Hit
GCGTTATTCCCATGACCCGCCGGGCAGCTTCCGGGAAACCAAA3350.10405277805387138No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA3270.10156793559288342No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGG3220.10001490905476593No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAACA303.593775E-430.83333436
TTGAAAC250.00549054129.59999816
TATACCT250.00549054129.5999985
ATACCTT250.00549054129.5999986
GGTAAAC358.8554766E-426.4285735
CGGTTAT358.8554766E-426.4285724
GTGATGT358.8554766E-426.4285724
CCGGTTA358.8554766E-426.4285723
GTGACAC358.8554766E-426.4285724
TTGGTAA451.320324E-424.66666633
TGGTAAA451.320324E-424.66666634
TAATACT400.001928184523.1250024
TGCTAGA400.001928184523.12500216
TGATGTT400.001928184523.12500225
AATACTG400.001928184523.1250025
TAGTACT400.001928184523.1250024
TATTAGA502.696252E-422.1999992
CCTATAC502.696252E-422.1999993
CTATACC603.7155707E-521.5833344
TAACGGT450.003819918520.55555530
Produced by FastQC (version 0.11.5)