FastQCFastQC Report
Fri 10 Feb 2017
ERR1630376.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630376.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828762
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT11040.13321074083995163No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9740.1175246934584356No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT8910.10750975551485227No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC8670.10461386984441853No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA8490.10244195559159323No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA2250.026.3111111
TGGACTA555.141431E-420.1818185
GACGGAC2500.019.247
ATAATAC609.2338515E-418.53
CAAGACG2700.018.54
ACCGTCC500.007033268518.49999822
TATATGG1054.7932735E-717.6190473
AAGACGG2950.017.5593225
CGCAAGA2850.017.5263162
GCGCAAG2900.017.2241381
TATAATA650.001579430917.0769232
CGGGAGT2350.016.5319144
CTAAGTC904.4435496E-516.4444454
AGCGAAA3050.016.3770517
CGGACCA3050.016.377059
CGGGTAA2600.016.36538524
GTGGGTA2600.016.3653859
GTGTTAT803.381141E-416.18751
TTCAGTA1151.2410965E-616.0869562
CGAAAGC3000.016.03333319
Produced by FastQC (version 0.11.5)