##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630376.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	828762
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.79055627550491	31.0	31.0	34.0	30.0	34.0
2	32.01991162722229	33.0	31.0	34.0	30.0	34.0
3	32.150454533388356	34.0	31.0	34.0	30.0	34.0
4	35.798645449477654	37.0	35.0	37.0	35.0	37.0
5	35.728141492973855	37.0	35.0	37.0	35.0	37.0
6	35.77859506106699	37.0	35.0	37.0	35.0	37.0
7	35.744692686199414	37.0	35.0	37.0	33.0	37.0
8	35.73909518052227	37.0	35.0	37.0	33.0	37.0
9	37.48774919699503	39.0	37.0	39.0	35.0	39.0
10	37.37189446427322	39.0	37.0	39.0	34.0	39.0
11	37.445780573916274	39.0	37.0	39.0	35.0	39.0
12	37.34896870271562	39.0	37.0	39.0	34.0	39.0
13	37.36292083855196	39.0	37.0	39.0	34.0	39.0
14	38.59272022607214	40.0	38.0	41.0	34.0	41.0
15	38.59213622246194	40.0	38.0	41.0	34.0	41.0
16	38.54851694455103	40.0	38.0	41.0	34.0	41.0
17	38.506076533431795	40.0	38.0	41.0	34.0	41.0
18	38.44474891464618	40.0	38.0	41.0	34.0	41.0
19	38.45581481776433	40.0	38.0	41.0	34.0	41.0
20	38.43516473969608	40.0	38.0	41.0	34.0	41.0
21	38.36841457499258	40.0	38.0	41.0	34.0	41.0
22	38.33234149249121	40.0	38.0	41.0	34.0	41.0
23	38.24571710575533	40.0	38.0	41.0	33.0	41.0
24	38.19159420919395	40.0	38.0	41.0	33.0	41.0
25	38.17739592307562	40.0	38.0	41.0	33.0	41.0
26	38.010757008646635	40.0	38.0	41.0	33.0	41.0
27	37.89029781770882	40.0	37.0	41.0	33.0	41.0
28	37.774474457081766	40.0	37.0	41.0	32.0	41.0
29	37.71203916202722	40.0	37.0	41.0	32.0	41.0
30	37.650522104054	40.0	37.0	41.0	32.0	41.0
31	37.607763145511015	40.0	37.0	41.0	32.0	41.0
32	37.52324551560038	40.0	37.0	41.0	32.0	41.0
33	37.40723874888086	40.0	37.0	41.0	31.0	41.0
34	37.34878770986121	40.0	37.0	41.0	31.0	41.0
35	37.20382087981833	39.0	36.0	41.0	31.0	41.0
36	37.11247378620159	39.0	36.0	41.0	31.0	41.0
37	37.06000999080556	39.0	36.0	41.0	31.0	41.0
38	36.951852280871954	39.0	36.0	41.0	30.0	41.0
39	36.87467692775489	39.0	36.0	41.0	30.0	41.0
40	36.753000258216474	39.0	35.0	41.0	30.0	41.0
41	36.644186147530895	39.0	35.0	41.0	30.0	41.0
42	36.59848665841339	39.0	35.0	41.0	30.0	41.0
43	35.28407552469829	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	4.0
14	0.0
15	0.0
16	8.0
17	15.0
18	31.0
19	92.0
20	172.0
21	325.0
22	581.0
23	1050.0
24	1716.0
25	2521.0
26	3845.0
27	5535.0
28	7773.0
29	10339.0
30	13618.0
31	17698.0
32	22942.0
33	29586.0
34	38883.0
35	52054.0
36	71707.0
37	106413.0
38	192810.0
39	249043.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.08494115318994	19.162920114580544	12.053038146053993	26.699100586175522
2	19.40448524425589	20.65647314910674	32.659919253054554	27.279122353582814
3	19.684420859064485	21.952261324722897	29.93151230389424	28.431805512318377
4	14.629652421322406	15.300653263542488	34.62067517574406	35.44901913939104
5	15.524119107777626	35.45782745830528	33.790159297844255	15.227894136072843
6	35.6399062698338	35.0528861120563	15.206174993544588	14.101032624565315
7	30.340797478648877	30.054587444887677	20.626307673373056	18.97830740309039
8	28.484776087706727	31.878995417260924	19.639051983561025	19.997176511471327
9	27.001358653027047	13.73856426814936	18.856921528738045	40.40315555008555
10	17.373142108349562	25.568015908065284	32.28466073492752	24.774181248657637
11	38.52963818321786	20.419613833645847	20.630892825684573	20.419855157451718
12	22.611919948067115	24.312046160417587	28.240315072360943	24.835718819154355
13	30.830564142660982	18.146705568064174	23.989396231970094	27.033334057304753
14	23.417217488253563	19.797963709726073	23.395739669531178	33.38907913248918
15	26.28112775440959	26.926186287498705	20.60760507841817	26.18508087967354
16	26.590142887825458	25.3291053402545	22.583805724683323	25.496946047236722
17	25.18660363288857	24.973273388499955	24.032955178929534	25.807167799681935
18	25.692780315699803	23.696067145935746	24.9171656036353	25.693986934729153
19	25.836367980192144	24.27114177532271	24.215999285681537	25.676490958803612
20	26.282455035341872	23.613293080522517	23.84158540087504	26.26266648326057
21	27.33860867172964	24.074945521150827	23.475376525468107	25.111069281651428
22	26.798646656096686	23.875129409890896	23.996756608049115	25.329467325963307
23	25.893561722183208	23.435075449887904	24.345831493239313	26.325531334689572
24	25.871239270140283	24.37527299755539	23.86342520530623	25.890062526998104
25	26.342785986809243	23.486839406246908	23.966711794218366	26.203662812725486
26	25.912626302846895	24.686580707127014	23.88562699544622	25.51516599457987
27	26.537292974340037	23.717665626561065	23.819866258346785	25.92517514075211
28	25.694710906146756	24.05720822141942	24.538407890323157	25.709672982110664
29	25.027691906723522	24.528392952379573	24.877829823278578	25.566085317618324
30	25.113844505418925	24.902686175283133	24.52441110958273	25.459058209715213
31	26.038959315219568	24.715539563831353	23.51085112493092	25.734649996018156
32	25.59926734092538	24.13189793933602	23.77111884956115	26.497715870177448
33	25.04265398268743	23.91591313308284	24.58763794672053	26.4537949375092
34	26.268820240310248	23.82626133920233	24.262092132602607	25.642826287884823
35	26.15636334677507	23.81938361073505	25.11010398642795	24.914149056061934
36	25.1140858292248	24.729295020765914	24.475181053185356	25.68143809682394
37	25.665390063733618	23.800922339586034	24.943952546086816	25.589735050593536
38	24.984132959764082	23.770032892434738	25.50551304234509	25.740321105456086
39	25.530972703864318	23.51109244873679	25.127237976644683	25.83069687075421
40	25.116016419671748	23.641889951518046	25.930001616869497	25.312092011940702
41	24.348486055103876	23.641769289615112	26.513160593753092	25.496584061527916
42	25.126514005227072	23.52460658186548	26.393343324138897	24.955536088768547
43	24.307219684300197	22.616867086087442	26.662057381974556	26.4138558476378
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	10.0
2	14.0
3	23.0
4	32.0
5	32.0
6	55.0
7	78.0
8	65.5
9	53.0
10	73.5
11	94.0
12	94.0
13	190.0
14	286.0
15	492.0
16	698.0
17	867.5
18	1037.0
19	1037.0
20	1544.5
21	2052.0
22	2712.5
23	3373.0
24	4516.0
25	5659.0
26	5659.0
27	6810.0
28	7961.0
29	9769.5
30	11578.0
31	14262.0
32	16946.0
33	16946.0
34	20180.5
35	23415.0
36	27986.0
37	32557.0
38	36753.5
39	40950.0
40	40950.0
41	45102.5
42	49255.0
43	50953.0
44	52651.0
45	55087.0
46	57523.0
47	57523.0
48	59463.0
49	61403.0
50	61716.0
51	62029.0
52	63614.5
53	65200.0
54	65200.0
55	63353.5
56	61507.0
57	59970.5
58	58434.0
59	56477.5
60	54521.0
61	54521.0
62	49958.0
63	45395.0
64	40702.5
65	36010.0
66	31286.5
67	26563.0
68	26563.0
69	22881.5
70	19200.0
71	16375.5
72	13551.0
73	10584.0
74	7617.0
75	7617.0
76	5880.5
77	4144.0
78	3438.0
79	2732.0
80	2347.5
81	1963.0
82	1963.0
83	1430.0
84	897.0
85	757.5
86	618.0
87	501.5
88	385.0
89	385.0
90	343.0
91	301.0
92	177.5
93	54.0
94	34.5
95	15.0
96	15.0
97	8.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	828762.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.244273078391686
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.27189723893913	45.710787819058794
2	11.212031223826292	12.612250918326803
3	3.201334835795623	5.401702521595716
4	1.3526000286124213	3.043040215004697
5	0.6948613262378153	1.954098509226655
6	0.4274827325715434	1.4426073328230582
7	0.3040621639460872	1.1971228767253304
8	0.20234430391170088	0.9104566628053427
9	0.1560324368790317	0.7898337890020056
>10	0.9683864316423079	10.40948504858739
>50	0.11341556990634401	4.492140522606768
>100	0.09038675055965466	9.985912121234055
>500	0.004949750623268399	1.9169320026554666
>1k	2.1520654883775648E-4	0.13362966034802268
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1104	0.13321074083995163	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	974	0.1175246934584356	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT	891	0.10750975551485227	No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATC	867	0.10461386984441853	No Hit
GTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAA	849	0.10244195559159323	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.2066190293473881E-4	0.0
5	0.0	0.0	0.0	1.2066190293473881E-4	0.0
6	0.0	0.0	0.0	1.2066190293473881E-4	0.0
7	0.0	0.0	0.0	1.2066190293473881E-4	0.0
8	0.0	0.0	0.0	1.2066190293473881E-4	0.0
9	0.0	0.0	0.0	3.6198570880421644E-4	0.0
10	0.0	0.0	0.0	3.6198570880421644E-4	0.0
11	0.0	0.0	0.0	3.6198570880421644E-4	0.0
12	0.0	0.0	0.0	4.8264761173895523E-4	0.0
13	0.0	0.0	0.0	4.8264761173895523E-4	0.0
14	0.0	0.0	0.0	4.8264761173895523E-4	0.0
15	0.0	0.0	0.0	4.8264761173895523E-4	0.0
16	0.0	0.0	0.0	4.8264761173895523E-4	0.0
17	0.0	0.0	0.0	4.8264761173895523E-4	0.0
18	0.0	0.0	0.0	4.8264761173895523E-4	0.0
19	0.0	0.0	0.0	4.8264761173895523E-4	0.0
20	0.0	0.0	0.0	4.8264761173895523E-4	0.0
21	0.0	0.0	0.0	4.8264761173895523E-4	0.0
22	0.0	0.0	0.0	7.239714176084329E-4	0.0
23	0.0	0.0	0.0	9.652952234779105E-4	0.0
24	0.0	0.0	0.0	0.0010859571264126493	0.0
25	0.0	0.0	0.0	0.001206619029347388	0.0
26	0.0	0.0	0.0	0.001809928544021082	0.0
27	0.0	0.0	0.0	0.007722361787823284	0.0
28	0.0	0.0	0.0	0.023529071072274067	0.0
29	0.0	0.0	0.0	0.04754078975628709	0.0
30	0.0	0.0	0.0	0.0802401654516013	0.0
31	0.0	0.0	0.0	0.1869052876459104	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	225	0.0	26.311111	1
TGGACTA	55	5.141431E-4	20.181818	5
GACGGAC	250	0.0	19.24	7
ATAATAC	60	9.2338515E-4	18.5	3
CAAGACG	270	0.0	18.5	4
ACCGTCC	50	0.0070332685	18.499998	22
TATATGG	105	4.7932735E-7	17.619047	3
AAGACGG	295	0.0	17.559322	5
CGCAAGA	285	0.0	17.526316	2
GCGCAAG	290	0.0	17.224138	1
TATAATA	65	0.0015794309	17.076923	2
CGGGAGT	235	0.0	16.531914	4
CTAAGTC	90	4.4435496E-5	16.444445	4
AGCGAAA	305	0.0	16.37705	17
CGGACCA	305	0.0	16.37705	9
CGGGTAA	260	0.0	16.365385	24
GTGGGTA	260	0.0	16.365385	9
GTGTTAT	80	3.381141E-4	16.1875	1
TTCAGTA	115	1.2410965E-6	16.086956	2
CGAAAGC	300	0.0	16.033333	19
>>END_MODULE