FastQCFastQC Report
Fri 10 Feb 2017
ERR1630375.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630375.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269322
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG20510.161582325052272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19060.15015890372970767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18230.1436199798002398No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT16660.13125117188546326No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA15500.1221124348274118No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC15450.12171852374732338No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14790.11651889749015615No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG14580.11486447095378478No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA12750.1004473254225484No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGACCG502.7023183E-422.1999995
TGCGTTA1350.021.92592637
AATGCGT1400.021.14285735
ATGCGTT1400.021.14285736
CTAGCAC2900.019.1379323
CGTTTAG1551.8189894E-1219.09677526
AAATGCG1551.8189894E-1219.09677534
GGTATCA8550.018.1754381
TAGGACA5850.017.7094024
ACCGTCG953.6069869E-617.5263168
GTACACT752.0674802E-417.2666666
CGTTATT752.0674802E-417.2666662
TTAGGAC5850.017.0769233
GCTTAGG5500.016.8181821
ATACCGC1005.8787646E-616.6527
CGAATTA1452.9795046E-916.58620815
CGTCGTA904.446544E-516.44444510
CGGTCCA904.446544E-516.44444510
ATACCGT1151.242368E-616.0869566
TAGCACT3000.016.0333334
Produced by FastQC (version 0.11.5)