FastQCFastQC Report
Fri 10 Feb 2017
ERR1630374.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630374.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1553180
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT94890.6109401357215519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61000.3927426312468613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT58760.37832060675517326No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.1887739991501307No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG22600.14550792567506665No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT18040.11614880438841602No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC17710.11402413113740842No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA16380.10546105409546866No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC2400.021.5833348
GACGGAC2450.019.6326547
GTACCGC851.245884E-619.5882366
CGGACCA2500.019.249
GTCCGGT500.007035968418.51
GTAAACG2350.018.10638227
CGAACGA1552.1827873E-1117.90322516
CTTATAC7650.017.89542437
TCTAGCG1258.583811E-917.7628
ACGCTTC2400.017.72916831
GCGAAAG2650.017.45283118
AAACGCT2600.017.07692329
TATACTG2100.016.7380945
ACGAACG1708.54925E-1116.3235315
CGGCCTT5450.016.29357724
TCGCCAT4550.016.26373513
CGAAAGC2850.016.2280719
GCGTTAT2400.016.18751
TTATACT2400.016.18754
TTCGGGC2750.016.14545435
Produced by FastQC (version 0.11.5)