##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630374.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1553180
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.800705649055487	31.0	31.0	34.0	30.0	34.0
2	32.010521639475144	33.0	31.0	34.0	30.0	34.0
3	32.13410744408246	34.0	31.0	34.0	30.0	34.0
4	35.79436639668293	37.0	35.0	37.0	35.0	37.0
5	35.7246674564442	37.0	35.0	37.0	33.0	37.0
6	35.784820175382116	37.0	35.0	37.0	35.0	37.0
7	35.76099550599415	37.0	35.0	37.0	33.0	37.0
8	35.76684350815746	37.0	35.0	37.0	35.0	37.0
9	37.49282633049614	39.0	37.0	39.0	35.0	39.0
10	37.38622117204702	39.0	37.0	39.0	34.0	39.0
11	37.456150607141474	39.0	37.0	39.0	35.0	39.0
12	37.385975225022214	39.0	37.0	39.0	34.0	39.0
13	37.39114140022406	39.0	37.0	39.0	34.0	39.0
14	38.603640273503395	40.0	38.0	41.0	34.0	41.0
15	38.60913867034085	40.0	38.0	41.0	34.0	41.0
16	38.56749571846148	40.0	38.0	41.0	34.0	41.0
17	38.562506599363886	40.0	38.0	41.0	34.0	41.0
18	38.52357228395936	40.0	38.0	41.0	34.0	41.0
19	38.57124029410628	40.0	38.0	41.0	34.0	41.0
20	38.556759036299724	40.0	38.0	41.0	34.0	41.0
21	38.50113187138645	40.0	38.0	41.0	34.0	41.0
22	38.46721693557733	40.0	38.0	41.0	34.0	41.0
23	38.40893457294067	40.0	38.0	41.0	34.0	41.0
24	38.37526944719865	40.0	38.0	41.0	34.0	41.0
25	38.35181949291132	40.0	38.0	41.0	34.0	41.0
26	38.18379324997746	40.0	38.0	41.0	33.0	41.0
27	38.06172368946291	40.0	38.0	41.0	33.0	41.0
28	37.98627847384076	40.0	37.0	41.0	33.0	41.0
29	37.920586796121505	40.0	37.0	41.0	33.0	41.0
30	37.84704799186186	40.0	37.0	41.0	33.0	41.0
31	37.76844151997837	40.0	37.0	41.0	33.0	41.0
32	37.66504011125562	40.0	37.0	41.0	33.0	41.0
33	37.566034844641315	40.0	37.0	41.0	32.0	41.0
34	37.503415573211086	40.0	37.0	41.0	32.0	41.0
35	37.37367401073926	40.0	36.0	41.0	32.0	41.0
36	37.26831532726406	40.0	36.0	41.0	31.0	41.0
37	37.20087433523481	39.0	36.0	41.0	31.0	41.0
38	37.0740545204033	39.0	36.0	41.0	31.0	41.0
39	36.99129849727655	39.0	36.0	41.0	31.0	41.0
40	36.8769337745786	39.0	35.0	41.0	30.0	41.0
41	36.745784777038075	39.0	35.0	41.0	30.0	41.0
42	36.68882099949781	39.0	35.0	41.0	30.0	41.0
43	35.47055524794293	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	5.0
10	7.0
11	10.0
12	13.0
13	8.0
14	5.0
15	11.0
16	10.0
17	30.0
18	59.0
19	143.0
20	266.0
21	510.0
22	906.0
23	1532.0
24	2682.0
25	4027.0
26	6338.0
27	9233.0
28	13382.0
29	18337.0
30	24765.0
31	32228.0
32	41377.0
33	53440.0
34	70483.0
35	93723.0
36	131147.0
37	202188.0
38	359149.0
39	487166.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.264515381346655	19.74381591315881	12.96359726496607	25.028071440528464
2	16.996870935757606	22.406095880709252	35.89712718422848	24.699905999304654
3	18.67349566695425	24.27696725427832	31.843894461685057	25.205642617082376
4	13.819389896856771	16.06774488468819	36.02563772389549	34.08722749455955
5	13.368379711301975	38.17336046047464	34.75997630667405	13.698283521549337
6	32.600793211347046	36.94175819930723	16.521072895607723	13.93637569373801
7	27.62847834764805	32.43436047335145	21.926048494057355	18.011112684943146
8	26.814857260587953	34.987702648759324	20.56934804723213	17.628092043420594
9	26.338350996021063	14.808071182992313	20.268095133854413	38.585482687132206
10	16.109980813556703	28.24650072753963	33.300905239573005	22.342613219330666
11	34.526906089442306	23.010082540336597	22.421097361542127	20.04191400867897
12	20.594264669903037	26.92798001519463	30.09870073011499	22.37905458478734
13	29.00159672414015	20.097026745129348	26.511286521845506	24.390090008884997
14	22.02816157818154	21.42089133262082	26.336097554694238	30.214849534503408
15	24.18380355142353	28.749146911497704	23.619219922996688	23.447829614082078
16	23.68469848955047	27.804761843443774	24.788240899316243	23.722298767689516
17	22.584697201869712	27.735355850577527	26.62563257317246	23.054314374380304
18	22.026616361271714	26.207587015027233	28.244569206402346	23.521227417298704
19	23.225060842915823	26.677526107727374	28.304575129733838	21.79283791962297
20	23.49405735330097	25.884958601063623	28.144065723225896	22.47691832240951
21	23.530434334719736	26.798696867072714	26.84550406263279	22.82536473557476
22	23.40115118659782	26.43563527730205	27.123771874476883	23.03944166162325
23	22.57291492293231	26.67707541946201	27.47930053181215	23.270709125793534
24	23.373594818372627	26.768758289444882	27.331024092506983	22.526622799675504
25	23.331358889504113	25.958678324469798	27.577357421548047	23.13260536447804
26	23.092816029050077	26.731415547457477	27.381050489962526	22.79471793352992
27	23.175356365649826	26.432416075406586	27.120359520467684	23.2718680384759
28	22.315893843598296	26.88638792670521	28.02276619580474	22.774952033891758
29	22.44929757014641	27.27359353069187	27.745592912605105	22.531515986556613
30	22.183906565884186	27.95252321044567	27.477497778750692	22.386072444919456
31	22.86663490387463	27.15815295072046	27.36109143821064	22.61412070719427
32	22.019984805367056	27.253441326826255	27.381823098417442	23.344750769389254
33	22.167874940444765	26.877309777359997	27.868823961163546	23.085991321031692
34	22.673611558222486	26.743712898698153	27.616631684672736	22.966043858406625
35	23.091850268481437	26.857157573494376	27.64547573365611	22.40551642436807
36	22.377638135953333	27.241015207509754	27.550187357550314	22.831159298986595
37	23.114770985977156	25.942904235182013	27.610837121260897	23.331487657579935
38	22.437772827360643	26.439305167462884	28.346231602261167	22.77669040291531
39	22.471445679187216	26.251883233108845	28.348163123398447	22.92850796430549
40	22.17972160342008	25.89274906965065	29.0399052266962	22.88762410023307
41	21.392691124016533	26.433574988088953	29.309223657270888	22.864510230623626
42	22.308875983466177	25.688522901402283	28.992711726908666	23.00988938822287
43	21.3123398447057	25.530910776600262	29.30272086944205	23.854028509251986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	266.0
1	260.0
2	254.0
3	700.5
4	1147.0
5	1147.0
6	1591.5
7	2036.0
8	1915.5
9	1795.0
10	2519.5
11	3244.0
12	3244.0
13	5802.5
14	8361.0
15	14556.0
16	20751.0
17	18839.5
18	16928.0
19	16928.0
20	18889.5
21	20851.0
22	21107.0
23	21363.0
24	24430.0
25	27497.0
26	27497.0
27	30369.5
28	33242.0
29	40519.5
30	47797.0
31	51298.5
32	54800.0
33	54800.0
34	59999.5
35	65199.0
36	70273.0
37	75347.0
38	83745.5
39	92144.0
40	92144.0
41	98226.5
42	104309.0
43	99462.0
44	94615.0
45	99664.0
46	104713.0
47	104713.0
48	107168.0
49	109623.0
50	110227.5
51	110832.0
52	113739.0
53	116646.0
54	116646.0
55	104064.5
56	91483.0
57	86014.5
58	80546.0
59	72785.0
60	65024.0
61	65024.0
62	59092.5
63	53161.0
64	46742.0
65	40323.0
66	34634.5
67	28946.0
68	28946.0
69	24984.0
70	21022.0
71	18257.0
72	15492.0
73	12575.0
74	9658.0
75	9658.0
76	7538.5
77	5419.0
78	4514.5
79	3610.0
80	2782.0
81	1954.0
82	1954.0
83	1475.5
84	997.0
85	840.0
86	683.0
87	660.0
88	637.0
89	637.0
90	511.5
91	386.0
92	219.5
93	53.0
94	34.5
95	16.0
96	16.0
97	11.5
98	7.0
99	5.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1553180.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.675418892701586
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.53428641788011	41.31784118343584
2	10.94639916152544	11.094267257540382
3	3.157957273567745	4.800924230498977
4	1.3144434245315766	2.6643988459557932
5	0.6867434345493749	1.7400505608801082
6	0.4326878307264824	1.3155982243103312
7	0.30334359321152243	1.0760444558087816
8	0.21068419184858472	0.8541207740797868
9	0.15429680452110156	0.70371496826209
>10	0.99237363736271	9.75942000417402
>50	0.12382545388492106	4.439120579688779
>100	0.12907068026136423	13.303507137874016
>500	0.010702752980193513	3.551015462170378
>1k	0.002803101971003063	1.994036229579937
>5k	3.82241177864054E-4	1.385940085740807
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9489	0.6109401357215519	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6100	0.3927426312468613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5876	0.37832060675517326	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2932	0.1887739991501307	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2260	0.14550792567506665	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1804	0.11614880438841602	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1771	0.11402413113740842	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1638	0.10546105409546866	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	6.438403790932153E-5	0.0	0.0	0.0
11	0.0	6.438403790932153E-5	0.0	6.438403790932153E-5	0.0
12	0.0	6.438403790932153E-5	0.0	6.438403790932153E-5	0.0
13	0.0	6.438403790932153E-5	0.0	2.575361516372861E-4	0.0
14	0.0	6.438403790932153E-5	0.0	3.219201895466076E-4	0.0
15	0.0	6.438403790932153E-5	0.0	5.150723032745722E-4	0.0
16	0.0	6.438403790932153E-5	0.0	8.369924928211798E-4	0.0
17	0.0	6.438403790932153E-5	0.0	0.0012232967202771088	0.0
18	0.0	6.438403790932153E-5	0.0	0.0013520647960957519	0.0
19	0.0	6.438403790932153E-5	0.0	0.0019315211372796456	0.0
20	0.0	6.438403790932153E-5	0.0	0.0023178253647355746	0.0
21	0.0	6.438403790932153E-5	0.0	0.0030260497817381116	0.0
22	0.0	6.438403790932153E-5	0.0	0.003734274198740648	0.0
23	0.0	6.438403790932153E-5	0.0	0.004893186881108435	0.0
24	0.0	6.438403790932153E-5	0.0	0.0072753962837533315	0.0
25	0.0	6.438403790932153E-5	0.0	0.008627461079849083	0.0
26	0.0	6.438403790932153E-5	0.0	0.010816518368766015	0.0
27	0.0	6.438403790932153E-5	0.0	0.020602892130982885	0.0
28	0.0	6.438403790932153E-5	0.0	0.05292367916146229	0.0
29	0.0	6.438403790932153E-5	0.0	0.09625413667443568	0.0
30	0.0	6.438403790932153E-5	0.0	0.1668834262609614	0.0
31	0.0	6.438403790932153E-5	0.0	0.3804452800061809	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGACC	240	0.0	21.583334	8
GACGGAC	245	0.0	19.632654	7
GTACCGC	85	1.245884E-6	19.588236	6
CGGACCA	250	0.0	19.24	9
GTCCGGT	50	0.0070359684	18.5	1
GTAAACG	235	0.0	18.106382	27
CGAACGA	155	2.1827873E-11	17.903225	16
CTTATAC	765	0.0	17.895424	37
TCTAGCG	125	8.583811E-9	17.76	28
ACGCTTC	240	0.0	17.729168	31
GCGAAAG	265	0.0	17.452831	18
AAACGCT	260	0.0	17.076923	29
TATACTG	210	0.0	16.738094	5
ACGAACG	170	8.54925E-11	16.32353	15
CGGCCTT	545	0.0	16.293577	24
TCGCCAT	455	0.0	16.263735	13
CGAAAGC	285	0.0	16.22807	19
GCGTTAT	240	0.0	16.1875	1
TTATACT	240	0.0	16.1875	4
TTCGGGC	275	0.0	16.145454	35
>>END_MODULE