FastQCFastQC Report
Fri 10 Feb 2017
ERR1630373.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630373.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1712789
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.22810749018121906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36040.210417044948327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30060.1755032289441373No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG26730.1560612544802658No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT19440.11349909416746605No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA19410.1133239412443681No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC18450.10771904770523397No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG17530.1023476913968971No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTA1001.2909368E-820.34999837
TAGGGCG555.1445083E-420.1818185
AATGCGT1153.0540832E-919.30434835
CGTTTAG1156.4103006E-817.69565226
GGTATCA15200.017.5263161
TCGCCAT4450.017.46067213
AAGACGG1705.456968E-1217.4117645
GACGGAC1851.8189894E-1217.07
TGCGTTT1251.6589911E-716.27999924
ATGCGTT1151.2429882E-616.08695636
AAATGCG1151.2429882E-616.08695634
TATACTG1403.4760888E-815.8571425
GTCGCCA5200.015.29807712
ACCGTCG1651.6463673E-814.5757578
ATCTCGC5600.014.53571411
GCTTAGG8150.014.3006131
GTAGCAC1304.446738E-614.2307693
CGACGGT1051.6563782E-414.0952397
CGAATTA1458.9165405E-714.03448315
ACGGACC1854.9021764E-914.08
Produced by FastQC (version 0.11.5)