##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630373.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1712789 Sequences flagged as poor quality 0 Sequence length 43 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.864093592380613 33.0 31.0 34.0 30.0 34.0 2 32.076527815159956 33.0 31.0 34.0 30.0 34.0 3 32.204982633587676 34.0 31.0 34.0 30.0 34.0 4 35.84430656665824 37.0 35.0 37.0 35.0 37.0 5 35.768047903156784 37.0 35.0 37.0 35.0 37.0 6 35.82615196617914 37.0 35.0 37.0 35.0 37.0 7 35.80528599845048 37.0 35.0 37.0 35.0 37.0 8 35.8072675618538 37.0 35.0 37.0 35.0 37.0 9 37.55763903201153 39.0 37.0 39.0 35.0 39.0 10 37.44433727680409 39.0 37.0 39.0 34.0 39.0 11 37.51269654347383 39.0 37.0 39.0 35.0 39.0 12 37.44381882415172 39.0 37.0 39.0 35.0 39.0 13 37.44295707177008 39.0 37.0 39.0 34.0 39.0 14 38.69805329202838 40.0 38.0 41.0 35.0 41.0 15 38.71446512092266 40.0 38.0 41.0 35.0 41.0 16 38.674072521483964 40.0 38.0 41.0 34.0 41.0 17 38.64358598753262 40.0 38.0 41.0 34.0 41.0 18 38.592383533523396 40.0 38.0 41.0 34.0 41.0 19 38.63686887293181 40.0 38.0 41.0 34.0 41.0 20 38.61859108156346 40.0 38.0 41.0 34.0 41.0 21 38.56053489367342 40.0 38.0 41.0 34.0 41.0 22 38.5401068082525 40.0 38.0 41.0 34.0 41.0 23 38.48916766747101 40.0 38.0 41.0 34.0 41.0 24 38.45907522759663 40.0 38.0 41.0 34.0 41.0 25 38.43498936529835 40.0 38.0 41.0 34.0 41.0 26 38.28762854035144 40.0 38.0 41.0 34.0 41.0 27 38.174416113134775 40.0 38.0 41.0 33.0 41.0 28 38.126067484085894 40.0 38.0 41.0 33.0 41.0 29 38.07913525834181 40.0 38.0 41.0 33.0 41.0 30 38.019023942820745 40.0 38.0 41.0 33.0 41.0 31 37.964742884266535 40.0 37.0 41.0 33.0 41.0 32 37.87356119171714 40.0 37.0 41.0 33.0 41.0 33 37.80290742175481 40.0 37.0 41.0 33.0 41.0 34 37.74721521448351 40.0 37.0 41.0 33.0 41.0 35 37.63126514707883 40.0 37.0 41.0 32.0 41.0 36 37.55212346646318 40.0 37.0 41.0 32.0 41.0 37 37.504520988866695 40.0 37.0 41.0 32.0 41.0 38 37.401444077466635 40.0 37.0 41.0 32.0 41.0 39 37.32577509547294 40.0 37.0 41.0 31.0 41.0 40 37.24348182992768 40.0 36.0 41.0 31.0 41.0 41 37.131745941852735 39.0 36.0 41.0 31.0 41.0 42 37.11367775014902 39.0 36.0 41.0 31.0 41.0 43 35.9030779623176 38.0 35.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 5.0 11 1.0 12 2.0 13 2.0 14 0.0 15 3.0 16 12.0 17 20.0 18 70.0 19 124.0 20 243.0 21 446.0 22 834.0 23 1500.0 24 2424.0 25 3766.0 26 6038.0 27 8787.0 28 12937.0 29 17953.0 30 24575.0 31 32928.0 32 42731.0 33 56143.0 34 74002.0 35 99187.0 36 138628.0 37 212077.0 38 401051.0 39 576298.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.340767018003966 19.096864821060855 12.409234295642953 26.153133865292222 2 17.708777905509667 21.42523101210949 35.359872115012415 25.506118967368423 3 19.327891526627038 23.32289616526029 30.649367785524078 26.699844522588595 4 14.634201877756103 15.704678159423022 34.94026409557745 34.72085586724343 5 14.020524419528616 37.105679683837295 34.71390813462721 14.159887762006878 6 34.04085383546952 35.518093588877555 16.130533299781817 14.310519275871108 7 28.73663948098686 31.143941256044965 21.56634588381873 18.553073379149446 8 26.70480718874304 34.73965561432261 20.31306833474526 18.242468862189096 9 26.560364411494934 14.647571884219246 19.489674443261837 39.302389261023976 10 16.209118577945095 27.43367688606127 33.73135862035546 22.62584591563818 11 34.927536316498994 22.24792429190052 21.95699528663484 20.867544104965642 12 20.934569290204454 25.80341186217333 29.917170182666986 23.34484866495523 13 29.562193591855156 20.003923425477392 25.68565071354382 24.748232269123633 14 22.5386781442431 20.87285707696628 25.42508154828178 31.163383230508835 15 24.798442773745045 27.957500894739518 22.729886751958357 24.514169579557084 16 24.660422270343865 27.0741463192489 23.924546456101716 24.340884954305523 17 23.403875200039234 27.05744840724689 25.62656579415211 23.91211059856176 18 22.771748300578764 26.085116146822525 27.07140225678703 24.071733295811683 19 24.190662130595186 25.88024561110563 27.121612761408443 22.807479496890743 20 24.471432266321187 25.264057627647073 26.964442205081884 23.300067900949852 21 23.925480605024905 26.072972210821067 25.794420678787638 24.207126505366393 22 24.081717012428268 25.600117702764326 26.05820098097314 24.259964303834273 23 23.47218484004743 26.135443420059328 26.224012414839187 24.16835932505405 24 24.353846270614767 26.017740655737516 26.29518288592465 23.333230187723064 25 24.39699227400456 25.43489011197526 26.276791828999368 23.891325785020808 26 24.09105850166016 26.013478601275462 26.063805874512276 23.831657022552104 27 24.155047702898607 25.514467923369427 25.984870290502798 24.345614083229165 28 23.478607113894356 25.905876322185627 26.52854496379881 24.086971600121206 29 23.514455078821733 26.24619845176493 26.432969852095034 23.806376617318303 30 23.26626338679195 27.03713066816753 26.26295474807463 23.433651196965886 31 24.084752996428634 26.118511970826532 26.236097966532945 23.560637066211893 32 23.158135648932824 26.34142325762251 26.36214968685577 24.138291406588902 33 23.430148138503924 25.853622366794742 26.606079324423497 24.110150170277834 34 23.7471749293112 25.628317323383094 26.615479197963086 24.009028549342624 35 23.91561365702372 25.903832871416157 26.55394213764801 23.626611333912116 36 23.572313927751754 26.226698092993356 26.502505562564917 23.698482416689973 37 24.401254328466614 25.215715420872044 26.242520240379875 24.140510010281478 38 23.719675920384823 25.832779168946086 26.921763276153687 23.525781634515404 39 23.727849723462725 25.527954698447967 26.92357318969237 23.820622388396938 40 23.220840395401883 25.079796752548038 27.620097980545182 24.079264871504897 41 22.74810265596054 25.64881021538555 27.784216269487956 23.81887085916596 42 23.378010951728438 24.884442858986134 27.54478222361307 24.192763965672363 43 22.930670386136295 24.856068085444267 27.679182899936887 24.53407862848255 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 164.0 1 158.5 2 153.0 3 362.5 4 572.0 5 572.0 6 826.5 7 1081.0 8 1015.0 9 949.0 10 1439.5 11 1930.0 12 1930.0 13 3207.5 14 4485.0 15 7466.5 16 10448.0 17 10747.5 18 11047.0 19 11047.0 20 12833.5 21 14620.0 22 16424.0 23 18228.0 24 21142.5 25 24057.0 26 24057.0 27 27538.5 28 31020.0 29 39521.0 30 48022.0 31 51957.5 32 55893.0 33 55893.0 34 61517.0 35 67141.0 36 71890.0 37 76639.0 38 86032.5 39 95426.0 40 95426.0 41 103153.5 42 110881.0 43 105860.0 44 100839.0 45 107146.5 46 113454.0 47 113454.0 48 117165.5 49 120877.0 50 125873.0 51 130869.0 52 137426.0 53 143983.0 54 143983.0 55 129679.0 56 115375.0 57 108080.5 58 100786.0 59 90568.5 60 80351.0 61 80351.0 62 74148.0 63 67945.0 64 60067.5 65 52190.0 66 45264.0 67 38338.0 68 38338.0 69 32581.0 70 26824.0 71 23333.5 72 19843.0 73 16128.5 74 12414.0 75 12414.0 76 9605.5 77 6797.0 78 5701.5 79 4606.0 80 3414.0 81 2222.0 82 2222.0 83 1616.0 84 1010.0 85 813.5 86 617.0 87 511.5 88 406.0 89 406.0 90 314.5 91 223.0 92 134.5 93 46.0 94 29.5 95 13.0 96 13.0 97 7.5 98 2.0 99 2.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 1712789.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.237287411415956 #Duplication Level Percentage of deduplicated Percentage of total 1 84.52996035840084 46.692057151925844 2 9.363934569586773 10.344766902439163 3 2.498945271439137 4.141048745516474 4 1.0219655028294743 2.258024088173756 5 0.5502802862711359 1.5197995164797493 6 0.35728173336614427 1.1841164275676754 7 0.23829942422063252 0.9214109649955011 8 0.17794824338957563 0.7863502611573271 9 0.13517636812870637 0.672009830780105 >10 0.906394782689536 9.659272226587666 >50 0.10831127146809347 4.211425439694907 >100 0.09903111422856034 11.3616829832287 >500 0.009194774884661933 3.3622027015068836 >1k 0.0032762990968335623 2.885832759946344 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 3907 0.22810749018121906 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 3604 0.210417044948327 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 3006 0.1755032289441373 No Hit GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG 2673 0.1560612544802658 No Hit GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT 1944 0.11349909416746605 No Hit TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA 1941 0.1133239412443681 No Hit CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC 1845 0.10771904770523397 No Hit CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG 1753 0.1023476913968971 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 5.838430769931381E-5 0.0 5 0.0 0.0 0.0 5.838430769931381E-5 0.0 6 0.0 0.0 0.0 5.838430769931381E-5 0.0 7 0.0 0.0 0.0 5.838430769931381E-5 0.0 8 0.0 0.0 0.0 5.838430769931381E-5 0.0 9 0.0 0.0 0.0 1.1676861539862762E-4 0.0 10 0.0 0.0 0.0 1.1676861539862762E-4 0.0 11 0.0 0.0 0.0 1.1676861539862762E-4 0.0 12 0.0 0.0 0.0 1.7515292309794143E-4 0.0 13 0.0 0.0 0.0 2.3353723079725525E-4 0.0 14 0.0 0.0 0.0 2.3353723079725525E-4 0.0 15 0.0 0.0 0.0 3.5030584619588287E-4 0.0 16 0.0 0.0 0.0 4.670744615945105E-4 0.0 17 0.0 0.0 0.0 5.254587692938243E-4 0.0 18 0.0 0.0 0.0 5.254587692938243E-4 0.0 19 0.0 0.0 0.0 5.254587692938243E-4 0.0 20 0.0 0.0 0.0 7.006116923917657E-4 0.0 21 0.0 0.0 0.0 8.757646154897072E-4 0.0 22 0.0 0.0 0.0 0.0011676861539862762 0.0 23 0.0 0.0 0.0 0.0019850664617766696 0.0 24 0.0 0.0 0.0 0.0025689095387698077 0.0 25 0.0 0.0 0.0 0.003094368308063632 0.0 26 0.0 0.0 0.0 0.0042036701543505944 0.0 27 0.0 0.0 0.0 0.009458257847288837 0.0 28 0.0 0.0 0.0 0.02422948769521523 0.0 29 0.0 0.0 0.0 0.04851735969812977 0.0 30 0.0 0.0 0.0 0.07794305077858393 0.0 31 0.0 0.0 0.0 0.18297642032964948 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCGTTA 100 1.2909368E-8 20.349998 37 TAGGGCG 55 5.1445083E-4 20.181818 5 AATGCGT 115 3.0540832E-9 19.304348 35 CGTTTAG 115 6.4103006E-8 17.695652 26 GGTATCA 1520 0.0 17.526316 1 TCGCCAT 445 0.0 17.460672 13 AAGACGG 170 5.456968E-12 17.411764 5 GACGGAC 185 1.8189894E-12 17.0 7 TGCGTTT 125 1.6589911E-7 16.279999 24 ATGCGTT 115 1.2429882E-6 16.086956 36 AAATGCG 115 1.2429882E-6 16.086956 34 TATACTG 140 3.4760888E-8 15.857142 5 GTCGCCA 520 0.0 15.298077 12 ACCGTCG 165 1.6463673E-8 14.575757 8 ATCTCGC 560 0.0 14.535714 11 GCTTAGG 815 0.0 14.300613 1 GTAGCAC 130 4.446738E-6 14.230769 3 CGACGGT 105 1.6563782E-4 14.095239 7 CGAATTA 145 8.9165405E-7 14.034483 15 ACGGACC 185 4.9021764E-9 14.0 8 >>END_MODULE