FastQCFastQC Report
Fri 10 Feb 2017
ERR1630372.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630372.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899378
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC52050.2740370795070808No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT46540.24502758271392006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46010.2422371955450679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT40930.21549159777569288No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA37980.19996019749623298No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA35710.18800891660322483No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG32400.17058215900152576No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31630.1665282002845142No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG27530.14494218633679026No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA26530.13967730488612587No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG26430.13915081674105945No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG26170.1377819475638867No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG26040.13709751297530035No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG26010.1369395665317804No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA24080.12677834533199817No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA23330.12282968424399988No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG21080.11098370098000504No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19930.104929087311741No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC19540.10287578354598188No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT19360.1019281048848623No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC19290.1015595631833158No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA19240.10129631911078259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17300.021.8150271
AAGACGG3350.020.9850735
ACGGACC3300.020.1818188
TAGTACG656.9051646E-519.9230754
CGGACCA3400.019.5882369
GACGGAC3500.019.5571427
GATAGAC701.2197289E-418.52
GCTTTAT11700.018.51
GCGCAAG3850.017.7792221
CGCAAGA3850.017.7792222
CAAGACG4150.017.3855424
CTTACAC2200.016.8181843
GCGAAAG4200.016.29761918
CGAAAGC4150.016.04819319
GAGCGAA4300.015.48837216
AGCGAAA4350.015.31034617
TTATTGA13900.015.3057574
CTTATAC5600.015.19642937
AGACCGT1853.0559022E-1015.06
AGACGGA4450.014.9662916
Produced by FastQC (version 0.11.5)