FastQCFastQC Report
Fri 10 Feb 2017
ERR1630371.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630371.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754186
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9790.129808827000236No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC9400.1246376888459876No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA9370.12423990898796848No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGT250.00549515929.64
TAGGGGT652.6808157E-622.769234
CCGGATA502.700908E-422.24
GGTATCA3100.020.8870961
GTTCTAA555.140839E-420.1818181
CTATACA555.140839E-420.1818184
CGGATAG555.140839E-420.1818185
TCTATAC656.89811E-519.9230773
TATACAG851.2441469E-619.5882365
ATAATAC701.218492E-418.53
ATAGCTA500.00703269318.55
ATACTAT609.232804E-418.56
CAAGACG1601.8189894E-1218.54
GCGCAAG1850.018.01
TAGAACA2700.017.8148144
CGGTCCT1054.791855E-717.6190512
CGCAAGA1705.456968E-1217.4117642
GAGTCGT650.001579253217.0769231
GACGGAC1851.8189894E-1217.07
AAGACGG1757.2759576E-1216.9142865
Produced by FastQC (version 0.11.5)