FastQCFastQC Report
Fri 10 Feb 2017
ERR1630370.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630370.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2077141
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84440.40652030844319187No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61920.2981020546992236No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53920.2595875773479027No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG53340.25679527773993194No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA41190.19830141526261338No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT41080.1977718411990327No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC39220.18881722521485061No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG36240.17447058240148358No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA36100.17379657904783546No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC30630.14746230515886982No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA30510.1468845879986No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA29630.14264799548995472No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC29400.14154070426610424No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC28860.13894097704489008No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT28310.13629310672698675No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT27540.13258608828192212No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT26500.1275792062262504No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA25580.1231500413308485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25280.12170574843017397No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG25200.12132060365666077No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA24860.11968373836922963No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC24850.11963559527254047No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC23690.11405099605659895No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC23510.11318442031619423No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC23510.11318442031619423No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC23200.11169198431883055No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG22020.10601109890951071No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA21090.10153379091741967No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT20970.10095607375714985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT951.6777449E-719.4736846
TCGCCAT9050.019.0110513
CGAACGA1705.456968E-1217.41176416
CGGCCTT8650.017.32369824
TCGCACG2100.016.73809622
AAGACGG3250.016.5076925
CCTTATA1352.2220775E-816.4444432
CGCAAGA3700.016.02
GTCGCCA10650.015.80751112
AGGTCGC10700.015.733644510
ACGAACG2003.6379788E-1215.72515
GCTTAGG14350.015.5993031
ATAACGA1902.7284841E-1115.57894812
CCGAAAC855.3667394E-415.23529417
TTAGAAC2109.094947E-1214.97619153
CTTAGGA15450.014.9676392
CCATTGT12250.014.9510216
CATTGTT12300.014.89024417
TTCCTCG9950.014.87437219
GAGGTCG11700.014.8632479
Produced by FastQC (version 0.11.5)