##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630370.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2077141
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.318049665381405	31.0	31.0	34.0	30.0	34.0
2	31.53347076582668	31.0	31.0	34.0	30.0	34.0
3	31.656693984664496	31.0	31.0	34.0	30.0	34.0
4	35.41842272623765	37.0	35.0	37.0	33.0	37.0
5	35.24092153589959	37.0	35.0	37.0	33.0	37.0
6	35.314272358015174	37.0	35.0	37.0	32.0	37.0
7	35.31064573854158	37.0	35.0	37.0	32.0	37.0
8	35.27483401463839	37.0	35.0	37.0	32.0	37.0
9	36.90638382276408	39.0	37.0	39.0	33.0	39.0
10	36.746540075998695	38.0	37.0	39.0	32.0	39.0
11	36.85516004931779	39.0	37.0	39.0	33.0	39.0
12	36.71792718934343	38.0	37.0	39.0	32.0	39.0
13	36.75835824337395	38.0	37.0	39.0	32.0	39.0
14	37.838108727332425	39.0	37.0	40.0	33.0	41.0
15	37.822761670969854	39.0	37.0	40.0	33.0	41.0
16	37.793680833414776	39.0	37.0	40.0	33.0	41.0
17	37.75904187534693	39.0	37.0	40.0	33.0	41.0
18	37.71721033863373	39.0	37.0	40.0	33.0	41.0
19	37.75217474403519	39.0	37.0	40.0	32.0	41.0
20	37.696635904832654	39.0	37.0	40.0	32.0	41.0
21	37.65026639982553	39.0	37.0	40.0	32.0	41.0
22	37.62954561101052	39.0	37.0	40.0	32.0	41.0
23	37.570196245705034	39.0	37.0	40.0	32.0	41.0
24	37.55822450185134	39.0	37.0	40.0	32.0	41.0
25	37.5148812719021	39.0	37.0	40.0	32.0	41.0
26	37.32929204132026	39.0	36.0	40.0	32.0	41.0
27	37.22055604313814	39.0	36.0	40.0	31.0	41.0
28	37.135757274060836	39.0	36.0	40.0	31.0	41.0
29	37.07111361241245	39.0	36.0	40.0	31.0	41.0
30	37.00055268274999	39.0	36.0	40.0	31.0	41.0
31	36.86576645494937	39.0	36.0	40.0	31.0	41.0
32	36.74285424051617	39.0	36.0	40.0	30.0	41.0
33	36.680023647889094	39.0	35.0	40.0	30.0	41.0
34	36.57919996764784	39.0	35.0	40.0	30.0	41.0
35	36.49281777212043	38.0	35.0	40.0	30.0	41.0
36	36.375749166763356	38.0	35.0	40.0	30.0	41.0
37	36.324269753473644	38.0	35.0	40.0	30.0	41.0
38	36.195531742910084	38.0	35.0	40.0	30.0	41.0
39	36.08723529120074	38.0	35.0	40.0	29.0	41.0
40	35.9925782602144	38.0	35.0	40.0	29.0	41.0
41	35.855651590334986	38.0	35.0	40.0	28.0	41.0
42	35.800507524525294	38.0	35.0	40.0	28.0	41.0
43	34.59445892214347	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	2.0
11	4.0
12	3.0
13	4.0
14	8.0
15	18.0
16	33.0
17	72.0
18	158.0
19	305.0
20	657.0
21	1160.0
22	1991.0
23	3357.0
24	5436.0
25	8225.0
26	12511.0
27	18132.0
28	24868.0
29	34013.0
30	45121.0
31	58456.0
32	73882.0
33	94759.0
34	123113.0
35	164102.0
36	230366.0
37	347156.0
38	533667.0
39	295556.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.39097923540096	20.243305582047633	13.353547014863217	25.01216816768818
2	17.49958235863622	22.551285637325535	35.820726662272804	24.128405341765436
3	19.32117270806363	24.23364615112792	31.70271060077289	24.74247054003556
4	14.821478176012123	16.25662388831572	35.248642244315626	33.67325569135654
5	13.419888202100868	38.404374089192785	34.68040927409358	13.49532843461277
6	33.33312471324768	36.321174152356534	16.212765527231902	14.132935607163885
7	27.872156969603896	32.21817873702363	21.543602480524914	18.366061812847565
8	26.242850148352954	35.76112550857164	20.253800777125868	17.742223565949544
9	26.097409853255026	15.08645778018921	19.817961322798983	38.99817104375678
10	16.10574342329192	28.538361141588364	33.398936326421754	21.956959108697966
11	34.748628042102105	22.82098326497816	22.143032177401533	20.287356515518205
12	20.589550733436006	26.87935002968022	29.629524428047976	22.901574808835797
13	29.110638131932305	20.230162516651493	26.23129580514756	24.427903546268645
14	22.0384653713927	21.55067951573822	25.64828290424194	30.762572208627148
15	24.410475745267174	28.857020298573854	23.3284596471785	23.404044308980467
16	24.222428809599347	27.75772082877378	24.100578631879106	23.919271729747763
17	23.11253785852766	27.7842476750495	25.714190803609384	23.389023662813454
18	21.854269883460006	26.362726459108938	27.858339900854105	23.92466375657695
19	23.605956456494766	26.713978492552986	27.821654861176974	21.858410189775274
20	23.822889250176082	25.698399867895343	27.884674174743076	22.594036707185502
21	23.402455586789728	27.081117747904454	26.231247662050865	23.285179003254957
22	23.514773431365516	26.229514510570056	26.64975560156966	23.605956456494766
23	22.499146663611185	26.934762733969432	26.896392685908175	23.669697916511204
24	24.357951626779307	26.746234367334715	26.521550535086448	22.374263470799526
25	23.785193205468477	25.964920051166484	27.08920578814823	23.16068095521681
26	23.81725650786345	26.261818528448476	26.64903345511932	23.27189150856875
27	23.543755575572387	26.07723789574227	26.563820173979526	23.81518635470582
28	22.547723048170536	26.600986644623546	27.49707410329872	23.354216203907196
29	23.081148559486333	27.168208609815125	26.76746547297463	22.98317735772391
30	22.475123258363297	28.02140056934026	26.67657130642552	22.826904865870926
31	23.531430942819963	26.723655254987506	27.010202966481334	22.7347108357112
32	22.34114102027739	27.183469971465584	26.85778192236348	23.617607085893543
33	22.7719735925486	26.445147440640763	27.264157801516603	23.518721165294025
34	22.91515116210214	26.407788397609984	27.141681763539406	23.535378676748472
35	23.485261713095067	26.575422660281607	26.8594669307476	23.079848695875725
36	22.89921579709803	27.189584144745112	26.929996567397207	22.981203490759654
37	24.08377669113459	25.723145419593568	26.63386837966224	23.5592095096096
38	23.12722150301785	26.697850555162116	27.577713790253043	22.597214151566984
39	23.233617746700872	26.15508528308863	27.583442818759053	23.027854151451443
40	22.983369930110666	25.5397202212079	28.306889132706925	23.170020715974506
41	22.149675924744635	26.719466805575546	28.29432378447106	22.836533485208758
42	23.228370149161755	25.497113580638004	27.885492607386787	23.389023662813454
43	22.29189063236439	25.682897790761437	28.04864956206632	23.976562014807854
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	166.0
2	204.0
3	534.5
4	865.0
5	865.0
6	1224.0
7	1583.0
8	1580.5
9	1578.0
10	2268.0
11	2958.0
12	2958.0
13	5210.0
14	7462.0
15	13862.0
16	20262.0
17	19183.0
18	18104.0
19	18104.0
20	19770.0
21	21436.0
22	23322.0
23	25208.0
24	28178.0
25	31148.0
26	31148.0
27	35223.0
28	39298.0
29	53237.0
30	67176.0
31	68265.0
32	69354.0
33	69354.0
34	76850.5
35	84347.0
36	88615.0
37	92883.0
38	109226.0
39	125569.0
40	125569.0
41	136634.0
42	147699.0
43	136283.5
44	124868.0
45	134157.0
46	143446.0
47	143446.0
48	149662.5
49	155879.0
50	159688.5
51	163498.0
52	170701.0
53	177904.0
54	177904.0
55	152759.5
56	127615.0
57	117857.5
58	108100.0
59	96979.0
60	85858.0
61	85858.0
62	77669.5
63	69481.0
64	60613.0
65	51745.0
66	44686.5
67	37628.0
68	37628.0
69	32037.5
70	26447.0
71	22883.5
72	19320.0
73	15704.5
74	12089.0
75	12089.0
76	9358.0
77	6627.0
78	5346.5
79	4066.0
80	3122.0
81	2178.0
82	2178.0
83	1644.0
84	1110.0
85	955.5
86	801.0
87	702.5
88	604.0
89	604.0
90	547.0
91	490.0
92	287.0
93	84.0
94	57.0
95	30.0
96	30.0
97	17.5
98	5.0
99	5.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2077141.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.471009954625764
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.00140140908533	38.10687941174867
2	10.704083952175598	9.948591837934039
3	3.0083974871371613	4.1940980871666635
4	1.2785226680287145	2.376569585327083
5	0.6791281464783979	1.5779885427732085
6	0.41045051769571583	1.1444430056231345
7	0.2983188493565366	0.970422475267008
8	0.20649647641825308	0.7676879848982227
9	0.14885444716223253	0.6225674846279781
>10	0.9935695902637145	9.006355467636217
>50	0.1214434292749324	3.8969593555844075
>100	0.11768256380466	11.614604275784634
>500	0.021792122147330405	6.884733478998014
>1k	0.009443252930509842	7.6650444243468145
>5k	4.150880409015316E-4	1.2230545822839307
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	8444	0.40652030844319187	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6192	0.2981020546992236	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5392	0.2595875773479027	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5334	0.25679527773993194	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	4119	0.19830141526261338	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	4108	0.1977718411990327	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3922	0.18881722521485061	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3624	0.17447058240148358	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	3610	0.17379657904783546	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	3063	0.14746230515886982	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	3051	0.1468845879986	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	2963	0.14264799548995472	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2940	0.14154070426610424	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	2886	0.13894097704489008	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2831	0.13629310672698675	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	2754	0.13258608828192212	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	2650	0.1275792062262504	No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA	2558	0.1231500413308485	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2528	0.12170574843017397	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	2520	0.12132060365666077	No Hit
GGTGTATTCTGAGGCCACATTGCTTTGCATGCCAATAAATAAA	2486	0.11968373836922963	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	2485	0.11963559527254047	No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC	2369	0.11405099605659895	No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC	2351	0.11318442031619423	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	2351	0.11318442031619423	No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC	2320	0.11169198431883055	No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG	2202	0.10601109890951071	No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA	2109	0.10153379091741967	No Hit
ATCTAGCACTTTCATATTTTAAAGCTGATATTTTAGCAATATT	2097	0.10095607375714985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	9.62861933783022E-5	4.81430966891511E-5	0.0	0.0	0.0
6	9.62861933783022E-5	4.81430966891511E-5	0.0	0.0	0.0
7	9.62861933783022E-5	4.81430966891511E-5	0.0	0.0	0.0
8	9.62861933783022E-5	4.81430966891511E-5	0.0	0.0	0.0
9	9.62861933783022E-5	4.81430966891511E-5	0.0	4.81430966891511E-5	0.0
10	9.62861933783022E-5	4.81430966891511E-5	0.0	1.444292900674533E-4	0.0
11	9.62861933783022E-5	9.62861933783022E-5	0.0	1.444292900674533E-4	0.0
12	9.62861933783022E-5	9.62861933783022E-5	0.0	1.444292900674533E-4	0.0
13	9.62861933783022E-5	9.62861933783022E-5	0.0	1.444292900674533E-4	0.0
14	9.62861933783022E-5	9.62861933783022E-5	0.0	1.444292900674533E-4	0.0
15	9.62861933783022E-5	9.62861933783022E-5	0.0	1.444292900674533E-4	0.0
16	9.62861933783022E-5	9.62861933783022E-5	0.0	3.370016768240577E-4	0.0
17	9.62861933783022E-5	9.62861933783022E-5	0.0	5.295740635806621E-4	0.0
18	9.62861933783022E-5	9.62861933783022E-5	0.0	6.258602569589643E-4	0.0
19	9.62861933783022E-5	9.62861933783022E-5	0.0	8.184326437155687E-4	0.0
20	9.62861933783022E-5	9.62861933783022E-5	0.0	0.0010110050304721731	0.0
21	1.444292900674533E-4	9.62861933783022E-5	0.0	0.0013480067072962308	0.0
22	1.444292900674533E-4	9.62861933783022E-5	0.0	0.0017812945774985905	0.0
23	1.444292900674533E-4	9.62861933783022E-5	0.0	0.002792299607970764	0.0
24	1.444292900674533E-4	9.62861933783022E-5	0.0	0.004477307992091052	0.0
25	1.444292900674533E-4	9.62861933783022E-5	0.0	0.0050068820556717145	0.0
26	1.444292900674533E-4	9.62861933783022E-5	0.0	0.006258602569589643	0.0
27	2.4071548344575548E-4	9.62861933783022E-5	0.0	0.009291617661006162	0.0
28	2.888585801349066E-4	9.62861933783022E-5	0.0	0.01973866964255195	0.0
29	2.888585801349066E-4	9.62861933783022E-5	0.0	0.03976619786523881	0.0
30	2.888585801349066E-4	9.62861933783022E-5	0.0	0.07033706426284975	0.0
31	2.888585801349066E-4	9.62861933783022E-5	0.0	0.18848022353802654	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	95	1.6777449E-7	19.473684	6
TCGCCAT	905	0.0	19.01105	13
CGAACGA	170	5.456968E-12	17.411764	16
CGGCCTT	865	0.0	17.323698	24
TCGCACG	210	0.0	16.738096	22
AAGACGG	325	0.0	16.507692	5
CCTTATA	135	2.2220775E-8	16.444443	2
CGCAAGA	370	0.0	16.0	2
GTCGCCA	1065	0.0	15.807511	12
AGGTCGC	1070	0.0	15.7336445	10
ACGAACG	200	3.6379788E-12	15.725	15
GCTTAGG	1435	0.0	15.599303	1
ATAACGA	190	2.7284841E-11	15.578948	12
CCGAAAC	85	5.3667394E-4	15.235294	17
TTAGAAC	210	9.094947E-12	14.9761915	3
CTTAGGA	1545	0.0	14.967639	2
CCATTGT	1225	0.0	14.95102	16
CATTGTT	1230	0.0	14.890244	17
TTCCTCG	995	0.0	14.874372	19
GAGGTCG	1170	0.0	14.863247	9
>>END_MODULE