FastQCFastQC Report
Fri 10 Feb 2017
ERR1630369.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630369.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991884
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG40360.4069024200410532No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG31990.3225175524557307No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA22650.22835331550866833No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT22330.22512713180170263No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT21450.21625512660754684No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC19120.1927644764912026No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC16300.16433373257356707No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC15860.15989772997648918No Hit
CAGTGATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAG14400.14517826681345802No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA13700.13812098995447047No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC13660.13771771699109978No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA13390.13499562448834743No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC13200.13308007791233653No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT12940.13045880365042686No Hit
ATCCTGATCAGATGAACGAGGACAAGCGCCATTCACAGGGCAC12940.13045880365042686No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC12830.1293498030011574No Hit
TATCTGGACTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGA12350.1245105274407088No Hit
GTACAAGGCAGCTGGCAACGTTCCCTTCAAGACACAGAGGAGA12180.12279661734638325No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC11750.11846143299014804No Hit
CATCAAAAGTTCCCAAAGAGGGCTTGCTCTCTCTTCACCTGCT11310.11402543039307016No Hit
GCCTTGTACCAGCATTACAAATAATCCAGCCACAAAGTAAATG11300.11392461215222749No Hit
TCATCAACCACTGCACAAAATCTTGGGCACGCCTGGAGTCCAG11260.11352133918885675No Hit
GTCCAGATACTTGCTGTAGTCACTGGTGAATGTGCCCTGTGAA11200.1129164297438007No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC11100.1119082473353739No Hit
TTCCCAGGCAGACCCACTCAGTGATCCTGATCAGATGAACGAG10920.11009351900020568No Hit
GTCTTGAAGGGAACGTTGCCAGCTGCCTTGTACCAGCATTACA10880.10969024603683494No Hit
GGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGATCTGG10830.10918615483262156No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC10510.10595997112565582No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT10480.10565751640312779No Hit
GATCAGGATCACTGAGTGGGTCTGCCTGGGAAGCTGAGAATGA10320.10404442454964491No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA10280.1036411515862742No Hit
GTGTTCATCTCATCAGAGAAAGAACCATCAGCATGTCTGCGGC10200.10283460565953277No Hit
GATGAACACCATTCTTGATAATCTTGCCGCCAGGGACTTTATA10070.10152396852857794No Hit
GGTAAAGGTCCCTTCAGCATGTCTCTCAAATTCATCGTGACGT9950.10031414963846579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3000.023.4333341
CGAATTA400.001931001423.12515
GTACTAG450.003825444520.5555551
CCGAATA450.003825444520.55555535
GCTTAGG9850.018.593911
GCCGACG1002.8739305E-718.512
CGAATAA500.00703421918.536
CCGAATT609.235598E-418.514
CTTAGGA10600.017.9764162
TTATTGT1351.1514203E-917.8148144
TCGCCAT5250.017.61904713
TCTTATA6650.017.24812137
GAGGTCG5500.017.1545479
CTAAGTC650.001579727717.0769234
TCTAGGG650.001579727717.0769233
TAGGACA11300.017.0265484
TTAGGAC11150.016.9237673
GATGTGC5600.016.84821336
GTCGCCA5550.016.66666612
ATTATCC1005.876156E-616.6500023
Produced by FastQC (version 0.11.5)