FastQCFastQC Report
Fri 10 Feb 2017
ERR1630368.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630368.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2559545
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT122440.4783662721303982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115820.4525023002135145No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC106590.4164412034170136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT97570.38120056494416No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT49860.19480024770027485No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44780.17495297015680522No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG37530.14662762326897943No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT36250.14162673443912882No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG35980.14057185945158224No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG35020.13682119282919425No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT32800.1281477762649221No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG31910.12467059575041659No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT29640.11580183196622837No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG28140.10994141536874717No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG28050.10958979037289832No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC26230.10247915156795445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37600.022.5345731
CAGGACA28650.019.178014
GACAGGC27650.018.6003637
TGCATCA26950.018.53432314
CCAGGAC29900.018.438133
GCATCAG27500.018.16363515
ACAGGCT29100.017.7371148
GGCCATC27600.017.69565226
GCTGCAT28350.017.48853512
ATCAAGC28500.017.3315830
TCCAGGA31600.017.3291132
CTGCATC28550.017.30122613
GGACAGG32000.017.1125016
CATCAAG29950.016.73956729
GCCATCA29600.016.687527
AGGCCAT29800.016.57550225
AGAGGCC29800.016.57550223
CCATCAA30650.016.23654228
AGCCCGT803.3845945E-416.18756
ATCAGAA31700.015.69873817
Produced by FastQC (version 0.11.5)