FastQCFastQC Report
Fri 10 Feb 2017
ERR1630367.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630367.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2840872
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG62320.21936926408511187No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT54100.1904344863126533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50650.17829032775851922No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG47270.16639257242142552No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC44780.15762765798670267No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA43890.15449481708433185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39700.13974582452148496No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC34440.12123038278387763No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC34220.12045597267317922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32890.11577431154941159No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA32450.11422549132801478No Hit
CTCCAGGCGTGCCCAAGATTTTGTGCAGTGGTTGATGAATACC32220.11341588075773917No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT32030.11274707202577237No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC31350.11035344077452275No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT31090.10943822882551554No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC30990.10908622422974355No Hit
CTTCAAGACACAGAGGAGAAATCCAGATCATTCTCAGCTTCCC29650.1043693626463987No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19250.018.6441571
GACGGAC3150.016.4444457
TCGCCAT10950.016.0502313
ACGTTAC1201.937844E-615.41666616
CTTATAC9400.015.15425437
GTCGCCA11850.015.14345912
TCTAGCG1252.9622734E-614.80000128
ACGGACC3750.014.7999998
CGGCCTT12150.014.769546524
CGACGTT1152.2119984E-514.47826114
GACGTTA1152.2119984E-514.47826115
ACCGTCG1551.212029E-714.3225818
ATCTCGC12150.014.31275711
TTCCTCG12600.014.24206419
TCCGTAC1051.6569848E-414.0952383
CGGACCA3700.014.09
TTGGCCG12300.013.98780431
GCGAAAG3750.013.81333318
AAGACGG4300.013.7674425
GAGGTCG12950.013.7142859
Produced by FastQC (version 0.11.5)