FastQCFastQC Report
Fri 10 Feb 2017
ERR1630366.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630366.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964755
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG49460.2517362215645208No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG36960.18811505760260186No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT28740.146277780181244No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA27830.14164615944481626No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT25440.12948179289529738No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23420.11920061279905127No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC20660.10515305979625958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10300.020.1165071
TAGGACG759.271258E-619.7333344
ACGGACC2250.018.0888888
TCTAGCG1950.018.0256428
GTTATAC1005.8820624E-616.653
AAGACGG2700.016.4444455
TTAGAGT1951.8189894E-1216.1282044
CGAGCCG3150.015.85714215
GCTTAGG13450.015.4052051
AACACCG855.3665554E-415.2352945
CTTATAC5350.015.21495237
TTTAGGC1101.4528323E-515.1363643
CTAGCGG2201.8189894E-1215.13636429
TACCGCA2950.015.05084728
GTAAACG2109.094947E-1214.9761927
GTATAGG2350.014.9574461
CGCAATA2350.014.95744636
TCGTACA750.00410578314.82
GACGGAC2750.014.7999997
CTTAGGA14250.014.5403512
Produced by FastQC (version 0.11.5)