FastQCFastQC Report
Fri 10 Feb 2017
ERR1630365.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630365.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299639
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9450.3153795066730299No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT9160.30570119376983634No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC5760.19223131835308488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5570.18589035472685464No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT4470.14917951268025859No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA4280.14283854905402835No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA4210.14050240456015406No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG3780.12615180266921194No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG3360.11213493570596618No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3360.11213493570596618No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG3160.10546023715203963No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT3100.10345782758586165No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG3090.10312409265816533No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG3050.10178915294738003No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC3020.10078794816429104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTC200.001839764937.04
TATACCA250.0054899429.65
ATACACT358.854032E-426.4285724
GACCATA509.061829E-625.90000211
CGACCAT551.8965784E-523.54545410
CCGACCA551.8965784E-523.5454549
CCTATCC502.695646E-422.23
ACGACGG502.695646E-422.26
TCTTATA2550.021.76470637
CCCCTAT450.003819307520.5555551
CCGGTGC450.003819307520.5555558
CGAACGA555.130905E-420.18181816
TAACGAA555.130905E-420.18181813
CATACTC555.130905E-420.1818183
ACGGTAT555.130905E-420.1818189
ACGAACG555.130905E-420.18181815
AGGCCCG851.2390301E-619.58823610
CAAGACG951.6658123E-719.4736844
GTCGCTC500.007023044418.526
GATAACG701.21538316E-418.511
Produced by FastQC (version 0.11.5)