##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630365.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	299639
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.558919232810148	31.0	31.0	34.0	30.0	34.0
2	31.75297941856701	31.0	31.0	34.0	30.0	34.0
3	31.879304763398622	33.0	31.0	34.0	30.0	34.0
4	35.59018018348746	37.0	35.0	37.0	33.0	37.0
5	35.50205080113069	37.0	35.0	37.0	33.0	37.0
6	35.57325648530398	37.0	35.0	37.0	33.0	37.0
7	35.54745877539305	37.0	35.0	37.0	33.0	37.0
8	35.52962398085697	37.0	35.0	37.0	33.0	37.0
9	37.19994059518287	39.0	37.0	39.0	34.0	39.0
10	37.10039414094961	39.0	37.0	39.0	33.0	39.0
11	37.188810535344196	39.0	37.0	39.0	34.0	39.0
12	37.0530371547095	39.0	37.0	39.0	33.0	39.0
13	37.095831984488	39.0	37.0	39.0	33.0	39.0
14	38.19676010132192	40.0	38.0	41.0	33.0	41.0
15	38.1985021976445	40.0	38.0	41.0	33.0	41.0
16	38.137061597455606	40.0	37.0	41.0	33.0	41.0
17	38.12742667009301	40.0	37.0	41.0	33.0	41.0
18	38.10430885165149	40.0	37.0	41.0	33.0	41.0
19	38.08584329810205	40.0	37.0	41.0	33.0	41.0
20	38.06049279299424	40.0	37.0	41.0	33.0	41.0
21	37.98522555475088	40.0	37.0	41.0	33.0	41.0
22	37.93593290593014	40.0	37.0	41.0	33.0	41.0
23	37.897009401312914	40.0	37.0	41.0	33.0	41.0
24	37.84172287319074	40.0	37.0	41.0	33.0	41.0
25	37.79634493507187	40.0	37.0	41.0	33.0	41.0
26	37.59606393026275	39.0	37.0	41.0	32.0	41.0
27	37.459552995437846	39.0	37.0	41.0	32.0	41.0
28	37.33527010836373	39.0	36.0	41.0	31.0	41.0
29	37.271520062475176	39.0	36.0	41.0	31.0	41.0
30	37.177844005620095	39.0	36.0	41.0	31.0	41.0
31	37.05173892584076	39.0	36.0	40.0	31.0	41.0
32	36.95357747155744	39.0	36.0	40.0	31.0	41.0
33	36.85871665570904	39.0	35.0	40.0	30.0	41.0
34	36.74728590070051	39.0	35.0	40.0	30.0	41.0
35	36.62438467622706	39.0	35.0	40.0	30.0	41.0
36	36.48935218713185	39.0	35.0	40.0	30.0	41.0
37	36.42536518944463	39.0	35.0	40.0	30.0	41.0
38	36.30013449517586	39.0	35.0	40.0	30.0	41.0
39	36.186447692056106	38.0	35.0	40.0	29.0	41.0
40	36.02553072196877	38.0	35.0	40.0	29.0	41.0
41	35.88298919700039	38.0	35.0	40.0	28.0	41.0
42	35.790467862995136	38.0	35.0	40.0	28.0	41.0
43	34.478248826087395	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	0.0
14	0.0
15	3.0
16	4.0
17	8.0
18	22.0
19	28.0
20	73.0
21	174.0
22	247.0
23	490.0
24	756.0
25	1246.0
26	1732.0
27	2402.0
28	3412.0
29	4544.0
30	5986.0
31	7750.0
32	10072.0
33	12853.0
34	16573.0
35	21723.0
36	29580.0
37	43679.0
38	73392.0
39	62887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.54706830552765	18.803627031194203	12.132933296400001	25.516371366878143
2	21.55393657033964	20.413898057328986	30.432620586772753	27.59954478555862
3	22.177019680348685	21.15679200638101	28.42120017754698	28.24498813572332
4	16.16912351195939	15.03409102286418	32.34926027653276	36.44752518864367
5	16.474157235873836	34.707097540707316	32.50077593370689	16.317969289711954
6	37.28486612223376	32.38797352814554	14.322234422087913	16.0049259275328
7	30.670239855292536	28.963519435053513	19.256171593150427	21.110069116503528
8	29.589606159411826	29.57625676230397	19.1039884661209	21.7301486121633
9	27.27982672482554	13.054375431769563	18.993522205053416	40.672275638351486
10	20.04178361294758	24.703059348082192	30.026798914694012	25.228358124276212
11	38.32511789186321	20.461288417061866	18.77492582741232	22.438667863662605
12	23.93513527945294	24.73943645520109	25.50469064440877	25.820737620937194
13	32.86788435417286	17.566471654223918	23.15653169313741	26.40911229846582
14	24.491805138850417	20.29976071205684	22.519097981237422	32.68933616785532
15	28.508304993675722	25.879141233284052	20.181284812724645	25.431268960315577
16	27.06823878066607	24.476453332176384	21.909364268336233	26.545943618821315
17	25.93320629157086	24.181097921165133	22.790424477454536	27.095271309809473
18	25.321136434175788	22.27947630315146	25.291100290683122	27.108286971989624
19	26.419791816152106	23.284685905372797	24.5568834497512	25.738638828723897
20	27.723694178661656	22.14431365743445	23.941476243079173	26.190515920824726
21	28.387159214921958	22.63623894085883	23.307713615383847	25.668888228835364
22	27.581523099463016	22.89087869069113	23.64979191627258	25.87780629357327
23	26.398099045851843	22.61754978490784	23.60607264074436	27.378278528495958
24	26.057355684673887	23.697849745860854	24.20345816132079	26.041336408144467
25	26.738508672102096	23.659136494248077	23.353435300478242	26.248919533171584
26	27.204068896238475	23.884407570443102	23.24931000303699	25.66221353028144
27	26.993482156862093	22.43232690003638	24.092991900253306	26.481199042848225
28	25.736636419157723	23.78528829691729	23.90176178668331	26.57631349724168
29	26.0386665287229	24.035923227617236	24.32427020514686	25.60114003851301
30	25.918521954752215	24.423055743744975	24.072300334736134	25.58612196676668
31	26.34904001148048	24.394020805035392	23.42452084007756	25.832418343406566
32	24.678362963432665	23.464235296473422	23.544331679120543	28.313070060973374
33	24.826207536402137	23.567025654203892	24.60861236354413	26.99815444584984
34	26.379409889900845	22.469705211938365	24.47445092261021	26.67643397555058
35	25.97091833840054	23.145852175451125	24.616622001808842	26.266607484339488
36	24.613618387459578	24.50515453595827	24.791165368994022	26.09006170758813
37	25.94922556810028	23.290359399143636	24.735765370996432	26.024649661759653
38	25.452627995688143	23.108807598476833	24.89428946165219	26.54427494418283
39	25.518707511372018	22.093252213496907	25.612153291126987	26.77588698400408
40	24.355975023278013	22.681626891025534	26.29530868812137	26.667089397575083
41	23.400825660211122	22.590517255764436	27.180373716372035	26.828283367652407
42	24.357309962988797	22.47738111527538	27.632250808472865	25.533058113262957
43	23.958496724391683	22.177353415276382	26.86966649868675	26.994483361645177
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	13.0
2	19.0
3	53.0
4	87.0
5	87.0
6	134.0
7	181.0
8	177.5
9	174.0
10	246.0
11	318.0
12	318.0
13	596.0
14	874.0
15	1410.5
16	1947.0
17	1654.5
18	1362.0
19	1362.0
20	1599.0
21	1836.0
22	1440.5
23	1045.0
24	1165.5
25	1286.0
26	1286.0
27	1652.0
28	2018.0
29	2340.0
30	2662.0
31	3149.5
32	3637.0
33	3637.0
34	4507.5
35	5378.0
36	6369.5
37	7361.0
38	8711.5
39	10062.0
40	10062.0
41	11079.5
42	12097.0
43	13336.5
44	14576.0
45	16790.0
46	19004.0
47	19004.0
48	21513.0
49	24022.0
50	23842.5
51	23663.0
52	24507.5
53	25352.0
54	25352.0
55	25221.5
56	25091.0
57	25088.5
58	25086.0
59	24706.5
60	24327.0
61	24327.0
62	22132.0
63	19937.0
64	17961.5
65	15986.0
66	13746.0
67	11506.0
68	11506.0
69	10200.0
70	8894.0
71	7237.5
72	5581.0
73	3787.5
74	1994.0
75	1994.0
76	1419.5
77	845.0
78	673.5
79	502.0
80	444.0
81	386.0
82	386.0
83	281.0
84	176.0
85	165.5
86	155.0
87	127.5
88	100.0
89	100.0
90	94.5
91	89.0
92	51.5
93	14.0
94	8.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	299639.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.871792069872335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.7490146393216	45.11709502706228
2	9.232876902833167	9.947832494323107
3	2.4660097376311674	3.9854509148384007
4	1.0998343676867524	2.3700019346928096
5	0.6490592837616493	1.748299338791392
6	0.42554070578294156	1.375478425152321
7	0.31380910404439305	1.1833821161898734
8	0.22380506386800816	0.9645423891905468
9	0.1845174998764054	0.8946259547934966
>10	1.336357611391565	14.509232246354149
>50	0.21920000891484606	8.197176166787232
>100	0.09749122209527147	8.705318127175325
>500	0.0024838527922362162	1.0015648646490765
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	945	0.3153795066730299	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	916	0.30570119376983634	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	576	0.19223131835308488	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	557	0.18589035472685464	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	447	0.14917951268025859	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	428	0.14283854905402835	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA	421	0.14050240456015406	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	378	0.12615180266921194	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	336	0.11213493570596618	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	336	0.11213493570596618	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	316	0.10546023715203963	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	310	0.10345782758586165	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	309	0.10312409265816533	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	305	0.10178915294738003	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC	302	0.10078794816429104	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.674698553926559E-4	0.0
10	0.0	0.0	0.0	6.674698553926559E-4	0.0
11	0.0	0.0	0.0	6.674698553926559E-4	0.0
12	0.0	0.0	0.0	6.674698553926559E-4	0.0
13	0.0	0.0	0.0	0.0010012047830889837	0.0
14	0.0	0.0	0.0	0.0010012047830889837	0.0
15	0.0	0.0	0.0	0.0013349397107853117	0.0
16	0.0	0.0	0.0	0.0016686746384816395	0.0
17	0.0	0.0	0.0	0.0020024095661779675	0.0
18	0.0	0.0	0.0	0.0020024095661779675	0.0
19	0.0	0.0	0.0	0.003337349276963279	0.0
20	0.0	0.0	0.0	0.004338554060052263	0.0
21	0.0	0.0	0.0	0.006674698553926558	0.0
22	0.0	0.0	0.0	0.009010843047800853	0.0
23	0.0	0.0	0.0	0.014016866963245772	0.0
24	0.0	0.0	0.0	0.01935662580638702	0.0
25	0.0	0.0	0.0	0.02069156551717233	0.0
26	0.0	0.0	0.0	0.02736626407109889	0.0
27	0.0	0.0	0.0	0.04505421523900427	0.0
28	0.0	0.0	0.0	0.08710481612874159	0.0
29	0.0	0.0	0.0	0.13182529644004953	0.0
30	0.0	0.0	0.0	0.21359035372564986	0.0
31	0.0	0.0	0.0	0.4582180557270582	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGGTC	20	0.0018397649	37.0	4
TATACCA	25	0.00548994	29.6	5
ATACACT	35	8.854032E-4	26.428572	4
GACCATA	50	9.061829E-6	25.900002	11
CGACCAT	55	1.8965784E-5	23.545454	10
CCGACCA	55	1.8965784E-5	23.545454	9
CCTATCC	50	2.695646E-4	22.2	3
ACGACGG	50	2.695646E-4	22.2	6
TCTTATA	255	0.0	21.764706	37
CCCCTAT	45	0.0038193075	20.555555	1
CCGGTGC	45	0.0038193075	20.555555	8
CGAACGA	55	5.130905E-4	20.181818	16
TAACGAA	55	5.130905E-4	20.181818	13
CATACTC	55	5.130905E-4	20.181818	3
ACGGTAT	55	5.130905E-4	20.181818	9
ACGAACG	55	5.130905E-4	20.181818	15
AGGCCCG	85	1.2390301E-6	19.588236	10
CAAGACG	95	1.6658123E-7	19.473684	4
GTCGCTC	50	0.0070230444	18.5	26
GATAACG	70	1.21538316E-4	18.5	11
>>END_MODULE