FastQCFastQC Report
Fri 10 Feb 2017
ERR1630364.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630364.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491861
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT206124.190614828173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131212.667623576579562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117372.3862432679151224No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59831.2164005684532824No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA47640.968566322599271No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29290.5954934422529943No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA26180.5322641965921266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18170.36941330985786636No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14450.29378218643071924No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA14080.28625973598232024No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12910.26247252780765296No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12660.25738979101819415No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA11910.24214158064981775No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA11350.23075625024142998No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10070.20473263787940088No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA9870.20066644844783385No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA9820.19964990108994207No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8070.1640707435637304No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7850.15959793518900667No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.1593946257174283No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA6880.13987691644590647No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA6790.1380471312017013No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6740.13703058384380953No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA6490.13194784705435073No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6310.12828827656594038No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5670.11527647038492583No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA5640.11466654197019076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG5260.10694078205021337No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA5160.10490768733442984No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5160.10490768733442984No Hit
GAACTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4930.10023156948812774No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4920.10002826001654938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTATA200.001840912237.02
CGATCGT303.5965117E-430.83333213
CCCAGTT250.005493324729.624
TACATTC250.005493324729.63
TCTCGTC250.005493324729.612
TGTTCAG250.005493324729.610
GTACTAG250.005493324729.61
CTTAACG358.862154E-426.4285717
ATCGTGC358.862154E-426.4285715
GTCAGAA358.862154E-426.4285724
TAAGAGT358.862154E-426.4285719
AGGAGCC509.077288E-625.9000027
ACAGAGG451.3217088E-424.6666683
ATTTTGG601.334809E-624.66666620
CGTGCTT400.001929624723.12500217
AGTTCTA400.001929624723.1250025
CCAAGCA400.001929624723.12500236
GTAGTGT400.001929624723.1250026
AGTATAC502.6990578E-422.22
CAGTCGG753.731766E-722.210
Produced by FastQC (version 0.11.5)