##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630364.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	491861
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.678960112714773	31.0	31.0	34.0	30.0	34.0
2	31.778504496188965	31.0	31.0	34.0	30.0	34.0
3	31.836703865522985	33.0	31.0	34.0	30.0	34.0
4	35.57553455142815	37.0	35.0	37.0	33.0	37.0
5	35.53042424587434	37.0	35.0	37.0	33.0	37.0
6	35.650769221385715	37.0	35.0	37.0	33.0	37.0
7	35.59420242710847	37.0	35.0	37.0	33.0	37.0
8	35.66063786313613	37.0	35.0	37.0	33.0	37.0
9	37.25671277047784	39.0	37.0	39.0	34.0	39.0
10	37.13806746214886	39.0	37.0	39.0	33.0	39.0
11	37.257924494928446	39.0	37.0	39.0	34.0	39.0
12	37.1678665313981	39.0	37.0	39.0	33.0	39.0
13	37.27254651212436	39.0	37.0	39.0	34.0	39.0
14	38.2108766501105	40.0	37.0	41.0	33.0	41.0
15	38.15555004360988	40.0	37.0	41.0	33.0	41.0
16	38.115719685032964	40.0	37.0	41.0	33.0	41.0
17	38.28878890580876	40.0	38.0	41.0	34.0	41.0
18	38.400045947940576	40.0	38.0	41.0	34.0	41.0
19	38.473156033920155	40.0	38.0	41.0	34.0	41.0
20	38.49677042904398	40.0	38.0	41.0	34.0	41.0
21	38.493645562465815	40.0	38.0	41.0	34.0	41.0
22	38.45008854127487	40.0	38.0	41.0	34.0	41.0
23	38.375374343564545	40.0	38.0	41.0	34.0	41.0
24	38.3489115827439	40.0	38.0	41.0	34.0	41.0
25	38.30172142129585	40.0	38.0	41.0	34.0	41.0
26	38.04393111061865	40.0	37.0	41.0	33.0	41.0
27	37.86137750299373	40.0	37.0	41.0	33.0	41.0
28	37.6706813510321	40.0	36.0	41.0	33.0	41.0
29	37.46507651551963	39.0	36.0	41.0	33.0	41.0
30	37.167333860582566	39.0	35.0	40.0	33.0	41.0
31	36.793388782603216	38.0	35.0	40.0	32.0	41.0
32	36.49985666682254	38.0	35.0	40.0	31.0	41.0
33	36.28385661802827	38.0	35.0	40.0	31.0	41.0
34	36.1187245990229	38.0	35.0	40.0	31.0	41.0
35	35.89319340220103	38.0	35.0	40.0	30.0	41.0
36	35.61820717641773	38.0	35.0	40.0	30.0	41.0
37	35.36991141806323	37.0	35.0	40.0	29.0	41.0
38	35.07041013619701	37.0	35.0	40.0	26.0	41.0
39	34.744078103366604	37.0	34.0	40.0	25.0	41.0
40	34.36000821370265	37.0	33.0	40.0	23.0	41.0
41	33.89178040137356	36.0	33.0	40.0	21.0	41.0
42	33.464466587104894	36.0	33.0	40.0	16.0	41.0
43	32.38476317496203	35.0	32.0	39.0	12.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	6.0
11	9.0
12	9.0
13	3.0
14	4.0
15	1.0
16	6.0
17	10.0
18	26.0
19	45.0
20	115.0
21	206.0
22	387.0
23	684.0
24	1254.0
25	2022.0
26	3003.0
27	4536.0
28	6406.0
29	8570.0
30	10983.0
31	13841.0
32	17472.0
33	22547.0
34	29939.0
35	37863.0
36	53007.0
37	89197.0
38	97634.0
39	92072.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.26322883904192	19.059653032055802	14.799099745659852	17.87801838324242
2	17.35104023291133	28.73067797609487	32.5876619614078	21.330619829586002
3	19.984101199322573	30.446609916216165	31.797601354854322	17.771687529606943
4	13.221621555683413	17.233324048867466	33.27810092688788	36.26695346856124
5	11.940975194211372	43.511479869312666	30.803011419893018	13.744533516582939
6	28.850630564326103	36.08499149149861	18.02135156070516	17.043026383470128
7	24.27250788332476	34.99281301017971	22.3599756841872	18.374703422308336
8	32.4363997145535	30.993309085290356	19.369090047798057	17.201201152358085
9	28.705467601619155	12.082885205373064	21.886671234352796	37.32497595865499
10	19.45773297740622	31.97366735724117	27.20951650974564	21.35908315560697
11	30.041617448832085	28.78760462813681	18.540197332173115	22.63058059085799
12	19.710853269521266	34.31904542136904	27.369114444934645	18.60098686417504
13	31.582093314981265	21.104743006662453	25.587310236021963	21.725853442334316
14	22.957298911684397	24.49716484941884	29.743159144555065	22.802377094341693
15	25.236398088077728	29.96456315910389	25.68896497181114	19.110073781007237
16	18.570897062381444	28.377122805020115	29.017954259435086	24.034025873163355
17	18.41963481552715	30.879659090678057	30.035111545741582	20.66559454805321
18	16.561996173715745	26.416202951646913	33.71440305289502	23.30739782174232
19	17.167655089547658	29.238545036097598	36.69491990623367	16.898879968121076
20	17.8556543413688	28.573316445093226	36.651614988787486	16.91941422475049
21	19.5217754609534	28.179099379702805	35.06356470628897	17.235560453054827
22	19.98084824777732	28.850630564326103	33.61823767283846	17.550283515058116
23	17.987602188423153	30.115825405958184	34.19766966683677	17.69890273878189
24	16.648809318079703	31.720547065126127	33.812398218195796	17.818245398598386
25	17.363848729620766	30.896127157875902	34.354624578895255	17.385399533608073
26	17.5569927276202	31.045966238429152	34.51035963412428	16.886681399826372
27	16.510762186878	31.962282026832785	34.13647351589169	17.39048227039753
28	16.348521228558475	32.59579434027093	35.49559733339297	15.560087097777625
29	17.283744797818894	32.25789399850771	34.50954639623796	15.948814807435435
30	16.15090442218432	33.92279526126284	34.53963619803156	15.38666411852129
31	16.849679075999113	32.91214387804685	34.18201483752524	16.056162208428802
32	15.114636045549453	33.50397774981143	34.33327708437953	17.048109120259586
33	14.940399828406806	33.765027111318034	35.14183885284663	16.15273420742852
34	15.776205066065414	33.4309896495148	34.0799534827929	16.712851801626883
35	15.617217059291141	34.027702948597266	34.30359390152909	16.0514860905825
36	15.419396943445404	34.001882645706814	33.10549118551786	17.47322922532992
37	15.485472521708369	33.88375984271979	33.520852435952435	17.109915199619405
38	15.088612433187427	33.748965663063345	33.82906959486522	17.333352308884013
39	15.035548661105475	33.13842731991355	34.7177352951342	17.10828872384678
40	15.220763589713354	33.478970684807294	34.98793358286182	16.312332142617528
41	14.836711997901848	33.15814833865665	34.894411225935784	17.110728437505717
42	14.321525796922302	33.64995395040469	34.62360300979342	17.404917242879595
43	14.52300548325645	33.06320281542956	34.40728173203405	18.00650996927994
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	133.0
2	208.0
3	850.5
4	1493.0
5	1493.0
6	2173.5
7	2854.0
8	2780.0
9	2706.0
10	3911.5
11	5117.0
12	5117.0
13	10611.0
14	16105.0
15	28130.0
16	40155.0
17	32526.0
18	24897.0
19	24897.0
20	26409.5
21	27922.0
22	18610.0
23	9298.0
24	9854.0
25	10410.0
26	10410.0
27	10412.5
28	10415.0
29	10919.0
30	11423.0
31	12232.5
32	13042.0
33	13042.0
34	14754.5
35	16467.0
36	17521.5
37	18576.0
38	19960.0
39	21344.0
40	21344.0
41	24435.5
42	27527.0
43	29472.5
44	31418.0
45	34470.0
46	37522.0
47	37522.0
48	39597.5
49	41673.0
50	36181.0
51	30689.0
52	27449.0
53	24209.0
54	24209.0
55	22246.5
56	20284.0
57	17829.5
58	15375.0
59	13481.5
60	11588.0
61	11588.0
62	9608.0
63	7628.0
64	5958.0
65	4288.0
66	3491.5
67	2695.0
68	2695.0
69	2228.0
70	1761.0
71	1400.0
72	1039.0
73	830.0
74	621.0
75	621.0
76	468.5
77	316.0
78	280.0
79	244.0
80	214.0
81	184.0
82	184.0
83	150.5
84	117.0
85	93.5
86	70.0
87	67.0
88	64.0
89	64.0
90	55.0
91	46.0
92	28.0
93	10.0
94	6.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	491861.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.152557436038137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.99476772989095	17.102423006761544
2	11.754675036851618	5.913202780127481
3	5.302968153799827	4.001496332097939
4	3.071891174691883	3.09063676834785
5	1.9897897350602947	2.5024150298371586
6	1.4044882833826686	2.1195883329615106
7	1.0991882508331194	1.935317692846883
8	0.8233215288495809	1.656691363417265
9	0.6842011418172251	1.5488467665624568
>10	5.133900573279366	25.516131147790862
>50	0.5208303538798962	8.979343288243278
>100	0.19571575409772493	8.815387176369686
>500	0.012131141782916835	2.106408856639009
>1k	0.00889617064080568	4.245568251754009
>5k	8.08742785527789E-4	1.2170588304462764
>10k+	0.002426228356583367	9.249484375796788
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20612	4.190614828173	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13121	2.667623576579562	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11737	2.3862432679151224	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5983	1.2164005684532824	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4764	0.968566322599271	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2929	0.5954934422529943	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	2618	0.5322641965921266	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1817	0.36941330985786636	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1445	0.29378218643071924	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1408	0.28625973598232024	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1291	0.26247252780765296	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1266	0.25738979101819415	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	1191	0.24214158064981775	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1135	0.23075625024142998	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1007	0.20473263787940088	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	987	0.20066644844783385	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	982	0.19964990108994207	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	807	0.1640707435637304	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	785	0.15959793518900667	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	784	0.1593946257174283	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	688	0.13987691644590647	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	679	0.1380471312017013	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	674	0.13703058384380953	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	649	0.13194784705435073	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	631	0.12828827656594038	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	567	0.11527647038492583	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	564	0.11466654197019076	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	526	0.10694078205021337	No Hit
GAACTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA	516	0.10490768733442984	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	516	0.10490768733442984	No Hit
GAACTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	493	0.10023156948812774	No Hit
GTACATGGGAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	492	0.10002826001654938	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.0330947157835242E-4	0.0
3	0.0	0.0	0.0	2.0330947157835242E-4	0.0
4	0.0	0.0	0.0	2.0330947157835242E-4	0.0
5	0.0	0.0	0.0	2.0330947157835242E-4	0.0
6	0.0	0.0	0.0	2.0330947157835242E-4	0.0
7	0.0	0.0	0.0	2.0330947157835242E-4	0.0
8	0.0	0.0	0.0	2.0330947157835242E-4	0.0
9	0.0	0.0	0.0	2.0330947157835242E-4	0.0
10	0.0	0.0	0.0	4.0661894315670484E-4	0.0
11	0.0	0.0	0.0	8.132378863134097E-4	0.0
12	0.0	0.0	0.0	8.132378863134097E-4	0.0
13	0.0	0.0	0.0	0.0012198568294701146	0.0
14	0.0	0.0	0.0	0.002033094715783524	0.0
15	0.0	0.0	0.0	0.0026430231305185816	0.0
16	0.0	0.0	0.0	0.005286046261037163	0.0
17	0.0	0.0	0.0	0.008945616749447506	0.0
18	0.0	0.0	0.0	0.010165473578917622	0.0
19	0.0	0.0	0.0	0.013825044067327964	0.0
20	0.0	0.0	0.0	0.015044900896798078	0.0
21	0.0	0.0	0.0	0.020940875572570298	0.0
22	0.0	0.0	0.0	0.03273282492411474	0.0
23	0.0	0.0	0.0	0.04676117846302106	0.0
24	0.0	0.0	0.0	0.06465241196191607	0.0
25	0.0	0.0	0.0	0.07034507716610994	0.0
26	0.0	0.0	0.0	0.08152709810291932	0.0
27	0.0	0.0	0.0	0.09901171265865763	0.0
28	0.0	0.0	0.0	0.13520079859960435	0.0
29	0.0	0.0	0.0	0.19375392641416986	0.0
30	0.0	0.0	0.0	0.3356639375758598	0.0
31	0.0	0.0	0.0	0.7451292133346616	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTATA	20	0.0018409122	37.0	2
CGATCGT	30	3.5965117E-4	30.833332	13
CCCAGTT	25	0.0054933247	29.6	24
TACATTC	25	0.0054933247	29.6	3
TCTCGTC	25	0.0054933247	29.6	12
TGTTCAG	25	0.0054933247	29.6	10
GTACTAG	25	0.0054933247	29.6	1
CTTAACG	35	8.862154E-4	26.42857	17
ATCGTGC	35	8.862154E-4	26.42857	15
GTCAGAA	35	8.862154E-4	26.42857	24
TAAGAGT	35	8.862154E-4	26.42857	19
AGGAGCC	50	9.077288E-6	25.900002	7
ACAGAGG	45	1.3217088E-4	24.666668	3
ATTTTGG	60	1.334809E-6	24.666666	20
CGTGCTT	40	0.0019296247	23.125002	17
AGTTCTA	40	0.0019296247	23.125002	5
CCAAGCA	40	0.0019296247	23.125002	36
GTAGTGT	40	0.0019296247	23.125002	6
AGTATAC	50	2.6990578E-4	22.2	2
CAGTCGG	75	3.731766E-7	22.2	10
>>END_MODULE