FastQCFastQC Report
Fri 10 Feb 2017
ERR1630363.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630363.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1909308
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG51620.2703597324266174No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42550.22285561051438532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37210.19488736233232146No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT33310.17446111366002762No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30710.16084361454516505No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG30660.16058173956218694No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA26930.14104586583201872No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC26840.14057449086265808No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT22810.11946736723462113No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC20960.10977799286443046No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC20890.10941136788826109No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT20630.10804961797677483No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA19450.10186936837849106No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC19250.10082186844657855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATA250.005497242329.629
CTAGCGG2750.022.87272629
CGCAATA2850.022.71929736
TCTAGCG2800.022.46428528
AAGACGG4250.022.25
ACGGACC4100.021.6585378
GACGGAC4100.021.6585377
CAAGACG4400.021.4431824
CGCAAGA4450.019.9550552
AGACGGA4500.019.7333326
CGGACCA4500.019.7333329
CGGTCCA3450.018.76811610
GCGCAAG5100.018.51
GGTATCA13950.018.3010751
TACCGTC2850.018.1754387
CGGCGCA4600.018.09782633
GCTTAGG12250.017.8204081
GCAATAC3600.017.47222337
GTATACG752.0681688E-417.2666661
CGAACGA1301.3946192E-817.07692316
Produced by FastQC (version 0.11.5)