FastQCFastQC Report
Fri 10 Feb 2017
ERR1630362.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630362.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283847
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60770.2660861257343421No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55610.24349266829170255No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38800.16988878852217335No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23660.1035971323823356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA1107.8159064E-716.81818215
AAGACGG2900.016.5862065
TCTTATA16050.016.48286437
TCGTTTA2600.016.36538530
TAACGCC2850.016.2280714
CTTATAC9350.016.224637
TCTAGCG1851.8189894E-1116.028
AATAACG2550.015.9607842
TAATACG957.064556E-515.5789484
CGCAATA2150.015.48837436
CTAGCGG2055.456968E-1215.34146329
ATAACGC3350.014.9104483
CCGCTTA1304.4483004E-614.23076925
GGTATCA34000.013.9294121
TTAGACT2009.858923E-1013.8749994
ATCGTAC950.001245732513.63157910
ATACCGT1907.1358954E-913.6315796
CTCTTAT25150.013.09343937
CGTACAC850.00940844913.0588233
CAAGACG4550.013.0109884
Produced by FastQC (version 0.11.5)