FastQCFastQC Report
Fri 10 Feb 2017
ERR1630361.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630361.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649646
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269654.150722085566601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT175212.6970072932027596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT152952.354359143287267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71071.0939804139485196No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA60520.9315842782069005No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA35370.5444503621972582No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA29780.45840349975217276No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22150.340954920064158No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA19770.3043195832807406No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15820.24351723861918642No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA13670.2104222915249228No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13650.2101144315519529No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA13540.20842120170061848No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12980.19980112245746146No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA12390.19071925325484956No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA11590.1784048543360538No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA10630.16362757563349886No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9420.14500204726882027No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9340.14377060737694067No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9340.14377060737694067No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9190.14146165757966647No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA9080.13976842772833203No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA8450.13007083857978036No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8150.12545293898523197No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG7400.11390818999886092No Hit
GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA7240.11144531021510178No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA7070.10882850044485765No Hit
GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6940.10682741062055334No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA6890.10605776068812861No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6730.10359488090436945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATGCG303.5977704E-430.83333419
ATACCGA303.5977704E-430.8333346
GGTCCTT250.005494606729.59999832
GGCCCCA250.005494606729.59999818
CAAAGGC250.005494606729.59999814
CGACGAG358.8652276E-426.4285724
TCACATT358.8652276E-426.4285726
GGATCAC358.8652276E-426.4285723
TCACTTA358.8652276E-426.4285730
CCCATTA706.5811037E-926.4285714
CTCTAAT1200.024.6666681
TAAACAG1150.024.13043237
CTATATC701.9202525E-723.7857134
CAGTCGG1750.023.25714110
GGTAAAC1200.023.12500235
GACGTGC400.00193028823.12500232
GGTCCAT400.00193028823.12500237
TACATAC802.7212081E-823.1250023
GTGCTAA400.00193028823.12500235
TGCGACG400.00193028823.12500222
Produced by FastQC (version 0.11.5)