##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1630361.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 649646 Sequences flagged as poor quality 0 Sequence length 43 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.62935660344249 31.0 31.0 34.0 30.0 34.0 2 31.755215917592043 31.0 31.0 34.0 30.0 34.0 3 31.808606533404348 33.0 31.0 34.0 30.0 34.0 4 35.566902590025954 37.0 35.0 37.0 33.0 37.0 5 35.52615116540393 37.0 35.0 37.0 33.0 37.0 6 35.65258925630266 37.0 35.0 37.0 33.0 37.0 7 35.610366261009844 37.0 35.0 37.0 33.0 37.0 8 35.65883573515422 37.0 35.0 37.0 33.0 37.0 9 37.26102215668225 39.0 37.0 39.0 34.0 39.0 10 37.12733396342008 39.0 37.0 39.0 33.0 39.0 11 37.25330256786003 39.0 37.0 39.0 34.0 39.0 12 37.14035028307725 39.0 37.0 39.0 33.0 39.0 13 37.242947697669194 39.0 37.0 39.0 34.0 39.0 14 38.20053690779286 40.0 37.0 41.0 33.0 41.0 15 38.139976233210085 40.0 37.0 41.0 33.0 41.0 16 38.08768467750129 40.0 37.0 41.0 33.0 41.0 17 38.24633569667173 40.0 38.0 41.0 34.0 41.0 18 38.359788253910594 40.0 38.0 41.0 34.0 41.0 19 38.45454909289059 40.0 38.0 41.0 34.0 41.0 20 38.473450155931076 40.0 38.0 41.0 34.0 41.0 21 38.48067562949668 40.0 38.0 41.0 34.0 41.0 22 38.45467685477937 40.0 38.0 41.0 34.0 41.0 23 38.3965113307863 40.0 38.0 41.0 34.0 41.0 24 38.38672292294573 40.0 38.0 41.0 34.0 41.0 25 38.351645357625536 40.0 38.0 41.0 34.0 41.0 26 38.12138303014257 40.0 37.0 41.0 33.0 41.0 27 37.9744168362462 40.0 37.0 41.0 33.0 41.0 28 37.82918543329752 40.0 37.0 41.0 33.0 41.0 29 37.646569054531234 39.0 36.0 41.0 33.0 41.0 30 37.388103674924494 39.0 36.0 41.0 33.0 41.0 31 37.02774895866364 39.0 35.0 40.0 32.0 41.0 32 36.760466469431044 38.0 35.0 40.0 32.0 41.0 33 36.57200382977806 38.0 35.0 40.0 31.0 41.0 34 36.434253424172546 38.0 35.0 40.0 31.0 41.0 35 36.23340711710686 38.0 35.0 40.0 31.0 41.0 36 36.00147310997066 38.0 35.0 40.0 30.0 41.0 37 35.77684000209345 38.0 35.0 40.0 30.0 41.0 38 35.530445812026855 38.0 35.0 40.0 29.0 41.0 39 35.2622505179744 38.0 35.0 40.0 27.0 41.0 40 34.954410555902754 37.0 34.0 40.0 25.0 41.0 41 34.56615295099177 37.0 34.0 40.0 23.0 41.0 42 34.218640613503354 37.0 33.0 40.0 21.0 41.0 43 33.187205031663396 35.0 33.0 40.0 18.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 6.0 11 12.0 12 6.0 13 7.0 14 6.0 15 1.0 16 4.0 17 8.0 18 26.0 19 39.0 20 91.0 21 189.0 22 449.0 23 814.0 24 1314.0 25 2220.0 26 3649.0 27 5222.0 28 7577.0 29 10365.0 30 13472.0 31 17353.0 32 21955.0 33 28339.0 34 37424.0 35 47846.0 36 69185.0 37 117077.0 38 137297.0 39 127690.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.949190790061046 18.790849170163444 14.57624614020559 18.68371389956992 2 17.341290487434698 27.430477521604075 33.37710075949055 21.851131231470678 3 19.177983086173082 30.279259781480995 31.51424006304972 19.028517069296203 4 12.163393602054041 17.297112581313513 34.28605732968417 36.253436486948274 5 10.895780163350501 44.5628850173787 31.614294554264937 12.927040265005866 6 28.389923127364746 36.91656686872543 17.85480092234848 16.838709081561344 7 25.388442320894768 34.42613361738547 22.443761679437724 17.74166238228204 8 32.90976932052225 31.22731456824178 18.509003364909503 17.35391274632646 9 29.258396111112823 11.85307074930039 21.018831794546568 37.86970134504023 10 19.137653429714028 32.16505604590808 28.24584466001484 20.451445864363052 11 30.915452415623278 28.56155506229546 18.421725062572538 22.101267459508716 12 20.11726386370423 34.215249535901094 27.25576698694366 18.41171961345102 13 31.743287882939324 20.741296028914206 25.63626959913553 21.87914648901094 14 23.58145820954797 23.23896398961896 29.18743438734326 23.992143413489806 15 25.275765570787783 29.56979647377187 25.52112996924479 19.633307986195558 16 19.907919082084703 27.06566345363475 28.964081976953604 24.062335487326944 17 19.03682928856639 30.14364746338775 30.24370195460297 20.57582129344289 18 17.261862614408464 25.776961606782773 33.403268857193 23.557906921615775 19 18.328289560776177 27.72463772577681 37.00569233090022 16.941380382546804 20 18.284727374600937 27.74803508372252 36.63579857337689 17.331438968299658 21 19.367163039563085 27.289631583970348 35.15453031343224 18.188675063034328 22 20.26842311043245 27.415392382928548 34.02914818224079 18.287036324398212 23 18.22854292953393 28.927446640170185 34.571597454613745 18.27241297568214 24 17.008955646613693 30.17628062052256 33.87414068585045 18.940623047013297 25 17.922837976374826 29.200364506207997 34.95811565067745 17.918681866739732 26 17.982870671103953 29.726958989973003 34.650871397653496 17.63929894126955 27 16.67154111623869 30.759213479341057 33.977735566754816 18.591509837665438 28 16.894585666655377 31.125720777161714 35.961431302586334 16.018262253596575 29 17.90652139780742 30.81570578438103 35.04216142329823 16.23561139451332 30 16.780985336629488 32.49862232662096 34.26881717119785 16.451575165551702 31 17.295111491489212 32.029135867841866 34.498172851060424 16.177579789608494 32 15.842012419071311 32.574355879971556 34.47708444291199 17.10654725804515 33 15.587720081398176 32.75953365371295 34.805571034070866 16.847175230818014 34 16.96031377088445 32.048531046138976 33.91370069237708 17.077454490599493 35 16.17834943954092 33.269195838964606 34.0884112270375 16.46404349445698 36 16.26455023197249 33.323379194207305 32.51478497520188 17.897285598618325 37 16.17465511986528 32.76907731287501 33.68265178266318 17.373615784596534 38 15.96869679794842 33.258882529870114 33.61538437856925 17.15703629361221 39 15.657604295262342 33.122192701871484 34.24926806291426 16.970934939951913 40 16.208365786905485 32.84188619648239 34.28482588979229 16.664922126819835 41 15.687774572613392 32.95148434685967 33.863519516782986 17.49722156374395 42 14.90734954113471 33.53441720567817 33.54134405506999 18.016889198117127 43 15.619121798641105 32.63007853507911 33.9738873170927 17.776912349187096 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 59.0 1 153.5 2 248.0 3 989.5 4 1731.0 5 1731.0 6 2623.0 7 3515.0 8 3233.5 9 2952.0 10 3912.0 11 4872.0 12 4872.0 13 10529.5 14 16187.0 15 31788.5 16 47390.0 17 38043.5 18 28697.0 19 28697.0 20 31320.5 21 33944.0 22 21997.5 23 10051.0 24 11012.0 25 11973.0 26 11973.0 27 12644.5 28 13316.0 29 14879.5 30 16443.0 31 17622.0 32 18801.0 33 18801.0 34 21415.0 35 24029.0 36 25929.0 37 27829.0 38 30060.0 39 32291.0 40 32291.0 41 36753.5 42 41216.0 43 44147.0 44 47078.0 45 49177.0 46 51276.0 47 51276.0 48 53728.0 49 56180.0 50 51131.0 51 46082.0 52 40285.5 53 34489.0 54 34489.0 55 30745.0 56 27001.0 57 21890.5 58 16780.0 59 14792.5 60 12805.0 61 12805.0 62 10948.5 63 9092.0 64 7264.0 65 5436.0 66 4430.5 67 3425.0 68 3425.0 69 2549.0 70 1673.0 71 1443.5 72 1214.0 73 1003.5 74 793.0 75 793.0 76 587.5 77 382.0 78 287.5 79 193.0 80 149.5 81 106.0 82 106.0 83 72.5 84 39.0 85 33.0 86 27.0 87 23.5 88 20.0 89 20.0 90 14.5 91 9.0 92 5.5 93 2.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 43 649646.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.54567963713253 #Duplication Level Percentage of deduplicated Percentage of total 1 67.19405026499417 13.805474302659505 2 11.713130899440213 4.813084700153932 3 5.123435621386341 3.1579340075543043 4 2.9906038905578396 2.457759578278541 5 1.9354095994932972 1.9882152798910129 6 1.409564054340947 1.7376270893104058 7 1.0720661642670783 1.5418429572588626 8 0.8370734649609891 1.375859459506637 9 0.6721123986346784 1.2428105420243534 >10 5.781105115414887 24.2897643913136 >50 0.794187058397235 11.342548537661003 >100 0.4480413782278741 15.37225967737776 >500 0.01648312762711242 2.3935357365519097 >1k 0.008990796887515867 3.253262862967912 >5k 0.0014984661479193113 2.0256310217561633 >10k+ 0.0022476992218789667 9.202389855734113 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 26965 4.150722085566601 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 17521 2.6970072932027596 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 15295 2.354359143287267 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7107 1.0939804139485196 No Hit GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 6052 0.9315842782069005 No Hit GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA 3537 0.5444503621972582 No Hit GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2978 0.45840349975217276 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2215 0.340954920064158 No Hit GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA 1977 0.3043195832807406 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1582 0.24351723861918642 No Hit GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA 1367 0.2104222915249228 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1365 0.2101144315519529 No Hit GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 1354 0.20842120170061848 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1298 0.19980112245746146 No Hit GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA 1239 0.19071925325484956 No Hit GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA 1159 0.1784048543360538 No Hit GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA 1063 0.16362757563349886 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 942 0.14500204726882027 No Hit GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 934 0.14377060737694067 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 934 0.14377060737694067 No Hit GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 919 0.14146165757966647 No Hit ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA 908 0.13976842772833203 No Hit GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA 845 0.13007083857978036 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 815 0.12545293898523197 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 740 0.11390818999886092 No Hit GAGTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAA 724 0.11144531021510178 No Hit GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA 707 0.10882850044485765 No Hit GTACATGGGAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAA 694 0.10682741062055334 No Hit GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA 689 0.10605776068812861 No Hit GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 673 0.10359488090436945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.539299864849472E-4 0.0 5 0.0 0.0 0.0 1.539299864849472E-4 0.0 6 0.0 0.0 0.0 1.539299864849472E-4 0.0 7 0.0 0.0 0.0 1.539299864849472E-4 0.0 8 0.0 0.0 0.0 1.539299864849472E-4 0.0 9 0.0 0.0 0.0 3.078599729698944E-4 0.0 10 0.0 0.0 0.0 6.157199459397888E-4 0.0 11 0.0 0.0 0.0 9.235799189096832E-4 0.0 12 0.0 0.0 0.0 0.0013853698783645248 0.0 13 0.0 0.0 0.0 0.0015392998648494718 0.0 14 0.0 0.0 0.0 0.001693229851334419 0.0 15 0.0 0.0 0.0 0.0020010898243043133 0.0 16 0.0 0.0 0.0 0.0036943196756387326 0.0 17 0.0 0.0 0.0 0.00492575956751831 0.0 18 0.0 0.0 0.0 0.005387549526973152 0.0 19 0.0 0.0 0.0 0.007388639351277465 0.0 20 0.0 0.0 0.0 0.010313309094491461 0.0 21 0.0 0.0 0.0 0.014007628770130193 0.0 22 0.0 0.0 0.0 0.02308949797274208 0.0 23 0.0 0.0 0.0 0.036943196756387325 0.0 24 0.0 0.0 0.0 0.05726195497240035 0.0 25 0.0 0.0 0.0 0.06264950449937351 0.0 26 0.0 0.0 0.0 0.06926849391822623 0.0 27 0.0 0.0 0.0 0.07804250314786822 0.0 28 0.0 0.0 0.0 0.09435908171527263 0.0 29 0.0 0.0 0.0 0.1279158187689911 0.0 30 0.0 0.0 0.0 0.21781093087620026 0.0 31 0.0 0.0 0.0 0.4697943187520588 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATGCG 30 3.5977704E-4 30.833334 19 ATACCGA 30 3.5977704E-4 30.833334 6 GGTCCTT 25 0.0054946067 29.599998 32 GGCCCCA 25 0.0054946067 29.599998 18 CAAAGGC 25 0.0054946067 29.599998 14 CGACGAG 35 8.8652276E-4 26.42857 24 TCACATT 35 8.8652276E-4 26.42857 26 GGATCAC 35 8.8652276E-4 26.42857 23 TCACTTA 35 8.8652276E-4 26.42857 30 CCCATTA 70 6.5811037E-9 26.42857 14 CTCTAAT 120 0.0 24.666668 1 TAAACAG 115 0.0 24.130432 37 CTATATC 70 1.9202525E-7 23.785713 4 CAGTCGG 175 0.0 23.257141 10 GGTAAAC 120 0.0 23.125002 35 GACGTGC 40 0.001930288 23.125002 32 GGTCCAT 40 0.001930288 23.125002 37 TACATAC 80 2.7212081E-8 23.125002 3 GTGCTAA 40 0.001930288 23.125002 35 TGCGACG 40 0.001930288 23.125002 22 >>END_MODULE