FastQCFastQC Report
Fri 10 Feb 2017
ERR1630360.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630360.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1333671
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26030.19517557178644507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20080.15056187020637024No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG17430.13069190227574864No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT15800.11846999747313994No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15560.11667045320772514No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA14710.11029706726771445No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAT303.5997864E-430.83333225
TCGACAT250.00549665629.624
TATACAG1350.021.9259265
AAGACGG3650.021.287675
ACGGACC3850.019.70138
GCGCAAG4000.019.4250011
CGCAAGA4100.019.4024412
CAAGACG3850.019.2207814
GACGGAC4000.018.9625027
CGGACCA4150.018.722899
ACGCAAT500.007035459418.511
CGCAATT609.2378765E-418.512
GGTATCA11500.018.3391281
ACGCGCG2250.018.08888821
CGCAATA2150.018.06976736
AGACGGA4750.017.5263166
GTATACT852.7236458E-517.4117644
TTCGGGC1705.456968E-1217.41176435
CTTATAC6000.017.26666637
GTACTAG752.0675792E-417.2666661
Produced by FastQC (version 0.11.5)