FastQCFastQC Report
Fri 10 Feb 2017
ERR1630359.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630359.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552097
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT96980.3800012303607582No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT91240.3575099222325797No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76780.3008506338121161No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56670.2220526884362154No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA45690.17902924536175546No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC45470.17816720916172074No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA41140.16120076940649197No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT41110.16108321901557818No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG32470.1272287064323966No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA30560.11974466487754971No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA25800.10109333618588949No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28650.024.6666661
TTTAGCG500.007037176818.526
AAGACGG3050.016.9836065
GCTTTAT15250.016.1344261
GTATCAA44150.015.9648922
CGCAAGA3700.014.52
CTTATAC19950.014.1879737
GACGGAC3350.013.805977
CGGACCA3400.013.60294159
CGAGCCG3150.013.50793715
ACGGACC3450.013.4057968
ATAACGC2355.638867E-1113.3829793
TTATTGA17850.013.3697474
GCGCAAG3750.013.321
ACCGACC850.00940884113.0588248
CGCAATA1857.39401E-813.036
TACCGTC2001.4648322E-812.9500017
CTTCGCG1153.579831E-412.86956537
CGAATTA1307.004336E-512.80769315
ATACCGT2602.3646862E-1112.8076936
Produced by FastQC (version 0.11.5)