##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630359.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2552097
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.951684830161238	33.0	31.0	34.0	30.0	34.0
2	32.16780670954121	33.0	31.0	34.0	30.0	34.0
3	32.30277297453819	34.0	31.0	34.0	30.0	34.0
4	35.920676604376716	37.0	35.0	37.0	35.0	37.0
5	35.84890699687355	37.0	35.0	37.0	35.0	37.0
6	35.90528416435582	37.0	35.0	37.0	35.0	37.0
7	35.89803443991353	37.0	35.0	37.0	35.0	37.0
8	35.90181486048532	37.0	35.0	37.0	35.0	37.0
9	37.6587688477358	39.0	37.0	39.0	35.0	39.0
10	37.577178688741064	39.0	37.0	39.0	35.0	39.0
11	37.62841655313258	39.0	37.0	39.0	35.0	39.0
12	37.57322586092927	39.0	37.0	39.0	35.0	39.0
13	37.57974442194008	39.0	37.0	39.0	35.0	39.0
14	38.841558530102894	40.0	38.0	41.0	35.0	41.0
15	38.851843797473215	40.0	38.0	41.0	35.0	41.0
16	38.808535882452745	40.0	38.0	41.0	35.0	41.0
17	38.80401802909529	40.0	38.0	41.0	35.0	41.0
18	38.77147106869371	40.0	38.0	41.0	35.0	41.0
19	38.7896898119468	40.0	38.0	41.0	35.0	41.0
20	38.783376964120094	40.0	38.0	41.0	35.0	41.0
21	38.73005101295131	40.0	38.0	41.0	35.0	41.0
22	38.68868228754628	40.0	38.0	41.0	34.0	41.0
23	38.64885621510468	40.0	38.0	41.0	34.0	41.0
24	38.62088705875991	40.0	38.0	41.0	34.0	41.0
25	38.609142599203714	40.0	38.0	41.0	34.0	41.0
26	38.459992312204434	40.0	38.0	41.0	34.0	41.0
27	38.343190325446095	40.0	38.0	41.0	34.0	41.0
28	38.29046544860952	40.0	38.0	41.0	34.0	41.0
29	38.24887455296566	40.0	38.0	41.0	34.0	41.0
30	38.19695489630684	40.0	38.0	41.0	34.0	41.0
31	38.13574366491556	40.0	38.0	41.0	34.0	41.0
32	38.06705270215043	40.0	37.0	41.0	33.0	41.0
33	37.98064846281313	40.0	37.0	41.0	33.0	41.0
34	37.92147712253884	40.0	37.0	41.0	33.0	41.0
35	37.808696142819024	40.0	37.0	41.0	33.0	41.0
36	37.73344900291799	40.0	37.0	41.0	33.0	41.0
37	37.67209945390007	40.0	37.0	41.0	33.0	41.0
38	37.58250803163046	40.0	37.0	41.0	33.0	41.0
39	37.496135530898705	40.0	37.0	41.0	32.0	41.0
40	37.38665379881721	40.0	37.0	41.0	32.0	41.0
41	37.289065031619096	40.0	36.0	41.0	32.0	41.0
42	37.25250490087171	40.0	36.0	41.0	32.0	41.0
43	36.12145071288435	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	8.0
11	3.0
12	5.0
13	2.0
14	5.0
15	7.0
16	13.0
17	22.0
18	60.0
19	136.0
20	247.0
21	487.0
22	886.0
23	1592.0
24	2824.0
25	4543.0
26	7152.0
27	10822.0
28	16192.0
29	23222.0
30	31776.0
31	43032.0
32	57716.0
33	76729.0
34	103605.0
35	143073.0
36	207142.0
37	322843.0
38	606854.0
39	891094.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.608529377997776	20.016127913633376	12.691719789647493	24.683622918721348
2	18.484132852317135	21.500867717802265	34.27075851740745	25.744240912473153
3	20.316586712809112	23.61258212364185	29.527952895207356	26.54287826834168
4	14.533851965658046	15.616922084074393	35.57317766526899	34.27604828499857
5	14.50293621284771	36.7579288718258	34.20022828285915	14.538906632467338
6	33.77497015199658	35.49845480011144	15.907389100022451	14.819185947869537
7	28.58993995917867	30.79052246054911	21.92784992106491	18.69168765920731
8	27.668736729050657	32.41761578811463	20.75281621349032	19.160831269344385
9	26.544014588787178	14.617077642425032	20.852812412694345	37.98609535609344
10	17.664571526865945	26.94321571633053	33.253712535221034	22.138500221582486
11	34.24352601017908	22.757990781698346	21.964525643030026	21.03395756509255
12	22.244804958432223	26.239323975538547	29.20500278790344	22.310868278125792
13	30.309506260929737	20.403887469794448	25.179254550277676	24.107351718998142
14	22.498321968169705	21.43676357129059	26.190423012918394	29.87449144762131
15	24.999715919888622	28.467256534528275	22.863629399666234	23.66939814591687
16	23.911395217344793	26.861714111963614	25.04187732676305	24.185013343928542
17	23.324348565121152	26.69741784892972	26.12428132629755	23.853952259651575
18	22.833536499592295	25.478890496717018	27.89584408429617	23.791728919394522
19	24.19187045006518	25.460239168025357	28.099911562922568	22.247978818986898
20	24.818492400563144	25.31408484865583	26.778880269833007	23.08854248094802
21	24.360986279126536	25.323841531101678	27.029771987506745	23.285400202265038
22	24.316944065997493	25.61473956514976	27.469018614888068	22.59929775396468
23	24.042150435504606	25.41533491869627	26.651612379936967	23.890902265862152
24	23.367489558586527	25.985101663455584	27.561334855219062	23.08607392273883
25	23.799330511340283	26.162485203344545	26.75415550427746	23.284028781037712
26	23.580882701558757	26.156725234189764	26.86504470637284	23.39734735787864
27	23.733815760137645	25.184857785577897	27.334932802319035	23.746393651965423
28	23.35412799748599	25.894940513624675	26.940433690412235	23.810497798477094
29	23.343901113476488	26.292417568767956	27.406089972285535	22.957591345470018
30	23.850700032169627	26.093679041196317	26.427091133291565	23.628529793342494
31	22.87287669708479	26.675671026610665	27.41737480981326	23.034077466491283
32	22.76261443040762	26.086234183105113	27.20100372360455	23.95014766288272
33	22.28559494407932	26.44715306667419	27.586177171165517	23.681074818080976
34	24.02988601138593	24.88937528628418	27.45569623725117	23.625042465078717
35	23.110485220585268	25.30691427481009	27.456597456914842	24.1260030476898
36	22.63052697448412	26.064526544249688	27.760504400890717	23.54444208037547
37	23.114795401585443	24.908575183466773	28.194108609508177	23.782520805439606
38	23.247000407899858	25.387867310686076	27.667443674750608	23.697688606663462
39	22.889568852594554	24.752193980087746	28.499191057393197	23.859046109924506
40	22.118555838590776	24.447229082593648	29.13893946820987	24.29527561060571
41	21.443855778209056	24.714225203822583	29.450095352958762	24.3918236650096
42	21.66806355714536	24.65066962580184	29.75776390944388	23.92350290760892
43	21.90657330030951	24.678137233812038	29.431013006167085	23.984276459711367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3079.0
1	2311.0
2	1543.0
3	2549.5
4	3556.0
5	3556.0
6	4235.0
7	4914.0
8	4196.0
9	3478.0
10	5057.0
11	6636.0
12	6636.0
13	10983.5
14	15331.0
15	19258.5
16	23186.0
17	23599.0
18	24012.0
19	24012.0
20	28287.5
21	32563.0
22	32508.0
23	32453.0
24	38510.0
25	44567.0
26	44567.0
27	50365.0
28	56163.0
29	63659.5
30	71156.0
31	78639.0
32	86122.0
33	86122.0
34	95013.0
35	103904.0
36	111937.5
37	119971.0
38	124115.0
39	128259.0
40	128259.0
41	133093.0
42	137927.0
43	139738.0
44	141549.0
45	145986.5
46	150424.0
47	150424.0
48	158429.0
49	166434.0
50	165890.5
51	165347.0
52	166119.0
53	166891.0
54	166891.0
55	161502.5
56	156114.0
57	153213.5
58	150313.0
59	148354.5
60	146396.0
61	146396.0
62	134077.0
63	121758.0
64	111437.0
65	101116.0
66	85825.0
67	70534.0
68	70534.0
69	62856.0
70	55178.0
71	45439.5
72	35701.0
73	24080.0
74	12459.0
75	12459.0
76	9035.5
77	5612.0
78	4377.0
79	3142.0
80	2505.5
81	1869.0
82	1869.0
83	1420.0
84	971.0
85	811.5
86	652.0
87	544.0
88	436.0
89	436.0
90	365.5
91	295.0
92	177.0
93	59.0
94	38.5
95	18.0
96	18.0
97	12.0
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2552097.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.629097864822384
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.4394921612571	46.68474069526087
2	11.167486394946831	12.648093599270332
3	2.9968075901903592	5.091195309207973
4	1.1213792355847088	2.5401077790202478
5	0.5415138557016425	1.533272056484281
6	0.3196960615838149	1.0862459735056884
7	0.21037131061002823	0.8339196275540367
8	0.1489949295603836	0.6749958757949824
9	0.11810230552501699	0.6019224315873607
>10	0.7795431492872387	8.639278750098867
>50	0.08258108735255594	3.2629797817035975
>100	0.05991851189699771	6.792887670119042
>500	0.009038142095815606	3.506333896023823
>1k	0.004797167727777874	4.837582484332396
>5k	2.780966798711811E-4	1.266444070036462
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9698	0.3800012303607582	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9124	0.3575099222325797	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7678	0.3008506338121161	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5667	0.2220526884362154	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4569	0.17902924536175546	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	4547	0.17816720916172074	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	4114	0.16120076940649197	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	4111	0.16108321901557818	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	3247	0.1272287064323966	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	3056	0.11974466487754971	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	2580	0.10109333618588949	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.918346363794166E-5	0.0
3	0.0	0.0	0.0	3.918346363794166E-5	0.0
4	0.0	0.0	0.0	7.836692727588332E-5	0.0
5	0.0	0.0	0.0	7.836692727588332E-5	0.0
6	0.0	0.0	0.0	7.836692727588332E-5	0.0
7	0.0	0.0	0.0	7.836692727588332E-5	0.0
8	0.0	0.0	0.0	7.836692727588332E-5	0.0
9	0.0	0.0	0.0	1.5673385455176663E-4	0.0
10	0.0	0.0	0.0	3.1346770910353327E-4	0.0
11	0.0	0.0	0.0	5.485684909311832E-4	0.0
12	0.0	0.0	0.0	9.012196636726583E-4	0.0
13	0.0	0.0	0.0	9.795865909485416E-4	0.0
14	0.0	0.0	0.0	0.001057953518224425	0.0
15	0.0	0.0	0.0	0.0012146873727761915	0.0
16	0.0	0.0	0.0	0.001998356645535025	0.0
17	0.0	0.0	0.0	0.002664475527380033	0.0
18	0.0	0.0	0.0	0.0032914109455870998	0.0
19	0.0	0.0	0.0	0.0038399794365182828	0.0
20	0.0	0.0	0.0	0.0042318140728977	0.0
21	0.0	0.0	0.0	0.00493711641838065	0.0
22	0.0	0.0	0.0	0.006700372282088024	0.0
23	0.0	0.0	0.0	0.008933829709450699	0.0
24	0.0	0.0	0.0	0.01296972646415869	0.0
25	0.0	0.0	0.0	0.015947669700642257	0.0
26	0.0	0.0	0.0	0.02288314276455793	0.0
27	0.0	0.0	0.0	0.053015226302135066	0.0
28	0.0	0.0	0.0	0.11884344521387706	0.0
29	0.0	0.0	0.0	0.21249192330855762	0.0
30	0.0	0.0	0.0	0.3265549859586058	0.0
31	0.0	0.0	0.0	0.6238007411160312	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2865	0.0	24.666666	1
TTTAGCG	50	0.0070371768	18.5	26
AAGACGG	305	0.0	16.983606	5
GCTTTAT	1525	0.0	16.134426	1
GTATCAA	4415	0.0	15.964892	2
CGCAAGA	370	0.0	14.5	2
CTTATAC	1995	0.0	14.18797	37
GACGGAC	335	0.0	13.80597	7
CGGACCA	340	0.0	13.6029415	9
CGAGCCG	315	0.0	13.507937	15
ACGGACC	345	0.0	13.405796	8
ATAACGC	235	5.638867E-11	13.382979	3
TTATTGA	1785	0.0	13.369747	4
GCGCAAG	375	0.0	13.32	1
ACCGACC	85	0.009408841	13.058824	8
CGCAATA	185	7.39401E-8	13.0	36
TACCGTC	200	1.4648322E-8	12.950001	7
CTTCGCG	115	3.579831E-4	12.869565	37
CGAATTA	130	7.004336E-5	12.807693	15
ATACCGT	260	2.3646862E-11	12.807693	6
>>END_MODULE