FastQCFastQC Report
Fri 10 Feb 2017
ERR1630358.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630358.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558316
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT62080.3983787627156494No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60640.3891380182196679No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC48730.31270936061748705No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT45840.29416369978874635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT42460.27247361895790073No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA35740.2293501446433201No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA35550.22813087974454474No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG33580.21548902789934776No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30390.19501821196727748No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG27140.17416236501454133No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG26570.1705045703182153No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA25650.16460076133467152No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG24790.15908198337179366No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG24340.15619425071679943No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA23770.1525364560204734No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG22900.14695350622081785No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA22560.14477166377037778No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG21420.1374560743777257No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT20800.1334774204975114No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC20280.13014048498507363No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA19240.12346661396019805No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA19120.12269655191886625No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC19080.12243986457175567No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC18750.12032219395809321No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC18300.11743446130309898No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA18290.11737028946632133No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG18140.1164077119146566No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT17240.11063224660466811No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT16860.10819371680711742No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG16760.10755199843934093No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA16650.10684610823478678No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA16490.10581935884634439No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG16180.10383003190623724No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC15890.1019690486396854No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG15750.10107064292479832No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG15590.10004389353635591No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20850.020.4964031
GGCTACG902.1532178E-618.51
GCTTTAT11350.018.2555071
CTTATAC10300.016.16504937
ATTACAC1504.6729838E-916.0333333
CTACGGC1403.475361E-815.85714330
CGAGCGT957.062255E-515.5789486
TACGGCT1201.936478E-615.41666631
TTATTGA11700.015.3376074
AAGACGG3050.015.1639355
ACGAGCG1101.452449E-515.1363645
CTATCCA1252.960198E-614.7999999
ACGCTCT1001.09376066E-414.79999933
CGAGCCG2006.184564E-1114.79999915
CTTTATT13500.014.7999992
TTTATTG12750.014.5098043
GCTACGA1152.210732E-514.478262
CGTCAAC908.276346E-414.3888883
CCGGAAC2850.014.28070313
GACGGAC3000.014.1833337
Produced by FastQC (version 0.11.5)