##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630358.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1558316
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.54975242505371	31.0	31.0	34.0	30.0	34.0
2	31.765939000818832	31.0	31.0	34.0	30.0	34.0
3	31.888066990263848	33.0	31.0	34.0	30.0	34.0
4	35.58222529961831	37.0	35.0	37.0	33.0	37.0
5	35.48751857774675	37.0	35.0	37.0	33.0	37.0
6	35.55413151119542	37.0	35.0	37.0	33.0	37.0
7	35.53310240028338	37.0	35.0	37.0	33.0	37.0
8	35.54469889290747	37.0	35.0	37.0	33.0	37.0
9	37.232862269270164	39.0	37.0	39.0	34.0	39.0
10	37.11393067901504	39.0	37.0	39.0	33.0	39.0
11	37.18027216559414	39.0	37.0	39.0	34.0	39.0
12	37.102162847586754	39.0	37.0	39.0	33.0	39.0
13	37.114700099338	39.0	37.0	39.0	33.0	39.0
14	38.26332399847014	40.0	38.0	41.0	33.0	41.0
15	38.263436299184505	40.0	38.0	41.0	33.0	41.0
16	38.19468836872624	40.0	38.0	41.0	33.0	41.0
17	38.18595843205101	40.0	38.0	41.0	33.0	41.0
18	38.12903159564556	40.0	37.0	41.0	33.0	41.0
19	38.131486168402304	40.0	38.0	41.0	33.0	41.0
20	38.13058519581394	40.0	38.0	41.0	33.0	41.0
21	38.04244132768964	40.0	37.0	41.0	33.0	41.0
22	37.998992502162594	40.0	37.0	41.0	33.0	41.0
23	37.954213394459146	40.0	37.0	41.0	33.0	41.0
24	37.89045225743688	40.0	37.0	41.0	33.0	41.0
25	37.88124359885928	40.0	37.0	41.0	33.0	41.0
26	37.66925963668473	40.0	37.0	41.0	32.0	41.0
27	37.52799111348404	40.0	37.0	41.0	32.0	41.0
28	37.422602347662476	39.0	37.0	41.0	32.0	41.0
29	37.38246735578663	39.0	36.0	41.0	32.0	41.0
30	37.30203245041442	39.0	36.0	41.0	31.0	41.0
31	37.22029100644542	39.0	36.0	41.0	31.0	41.0
32	37.15367871471511	39.0	36.0	41.0	31.0	41.0
33	37.0158838130392	39.0	36.0	40.0	31.0	41.0
34	36.968539757019755	39.0	36.0	40.0	31.0	41.0
35	36.800181734641754	39.0	35.0	40.0	30.0	41.0
36	36.70214385272307	39.0	35.0	40.0	30.0	41.0
37	36.62444395103432	39.0	35.0	40.0	30.0	41.0
38	36.50091188180061	39.0	35.0	40.0	30.0	41.0
39	36.402736030432855	39.0	35.0	40.0	30.0	41.0
40	36.258830044740606	39.0	35.0	40.0	30.0	41.0
41	36.14045610774708	38.0	35.0	40.0	29.0	41.0
42	36.08598897784532	38.0	35.0	40.0	29.0	41.0
43	34.8035424137338	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	6.0
12	2.0
13	1.0
14	2.0
15	13.0
16	18.0
17	49.0
18	105.0
19	173.0
20	389.0
21	743.0
22	1423.0
23	2362.0
24	3811.0
25	5856.0
26	8434.0
27	12266.0
28	17094.0
29	22845.0
30	30557.0
31	38950.0
32	49389.0
33	62670.0
34	81278.0
35	108085.0
36	149572.0
37	225184.0
38	393386.0
39	343647.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.223903239137634	19.188598461416042	12.496695150405952	25.090803149040376
2	20.12287623306184	21.21424666113933	31.353589387518323	27.3092877182805
3	21.70965324106279	22.337189632911425	28.537793361551827	27.41536376447396
4	15.887406662063409	15.039889213740986	33.15861481240005	35.91408931179556
5	15.829395321616413	35.421955495547756	33.10188690868861	15.646762274147221
6	36.206199512807416	33.442575190141156	14.753105275181671	15.598120021869763
7	30.244186673306313	29.384348232322584	20.56155490927386	19.809910185097245
8	29.23675300773399	30.167052125499577	19.87754730106089	20.718647565705545
9	26.786608107726543	14.270212203429855	19.795664037332607	39.147515651510986
10	18.947119839621745	26.073081454595858	31.27966343155047	23.700135274231926
11	36.238606290380126	21.848713611359955	20.542239186403783	21.370440911856132
12	24.119562399410647	25.063979321267315	26.75753826566627	24.058920013655765
13	31.591474386453065	18.973430292700584	23.93051216826369	25.50458315258266
14	23.758467473862808	20.47543630431825	24.194964307624385	31.571131914194556
15	26.290623981272095	27.733271043870435	21.148085497421576	24.828019477435898
16	25.51626242687619	25.478786074198045	23.569609758226186	25.435341740699574
17	25.471598828478946	25.348003870845194	24.007646716070425	25.172750584605435
18	24.30405643014639	23.707579207298135	26.51400614509509	25.474358217460384
19	26.20822734284959	23.88924967721566	26.523696092448517	23.378826887486234
20	26.60301248270569	23.62434833499752	24.610733638106776	25.16190554419001
21	26.104846513800794	23.707001660767133	25.605846311017793	24.58230551441428
22	26.243393509403738	24.08105929734406	25.655130281663023	24.020416911589177
23	25.29320112223708	23.913121600496947	24.766093654945468	26.027583622320506
24	24.775526914951783	24.66797491651244	26.248912287366622	24.30758588116916
25	25.322527651644467	24.54656180132913	24.88968861257922	25.241221934447182
26	25.327276367566014	24.912084583614618	25.273115337325674	24.48752371149369
27	25.68317337433486	23.32306156132646	25.691130682095288	25.302634382243394
28	24.944170502003445	24.555930889498665	25.043701020845578	25.456197587652312
29	25.22517897525277	24.99152931754535	25.637226339202062	24.146065367999814
30	25.55027350036835	24.435544523703793	24.594048960544587	25.42013301538327
31	24.024010534448724	25.46608005051607	25.98600027208859	24.523909142946618
32	24.267029280325687	24.31689079750192	25.34344767043398	26.072632251738415
33	23.461223525908736	25.278698287125334	25.92368941857749	25.33638876838844
34	26.394133153994442	23.23290013065386	25.301350945507846	25.07161576984386
35	24.870757920729815	23.499790799812104	25.709098796393032	25.920352483065052
36	23.64212393378493	24.95193529425354	26.53280849327094	24.873132278690587
37	24.580572874821282	23.451405234881754	26.968214405807295	24.999807484489665
38	24.865239142766935	23.87423346740969	26.121531191362983	25.13899619846039
39	24.48534186904325	23.167701544487766	26.967508515602738	25.379448070866246
40	23.501010064710883	23.045775054610232	27.569761203760983	25.883453676917906
41	22.207434178947018	23.767002328154238	28.195821643363733	25.829741849535008
42	23.14902753998547	23.25888972454881	28.74641600291597	24.845666732549752
43	23.787280628575978	23.30605602458038	28.03776640938038	24.868896937463262
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	896.0
1	780.5
2	665.0
3	1083.5
4	1502.0
5	1502.0
6	1891.0
7	2280.0
8	2029.0
9	1778.0
10	2507.5
11	3237.0
12	3237.0
13	5737.5
14	8238.0
15	11293.0
16	14348.0
17	13133.0
18	11918.0
19	11918.0
20	13906.5
21	15895.0
22	14371.5
23	12848.0
24	15252.5
25	17657.0
26	17657.0
27	20111.0
28	22565.0
29	25798.5
30	29032.0
31	32507.0
32	35982.0
33	35982.0
34	40739.5
35	45497.0
36	50595.5
37	55694.0
38	59052.0
39	62410.0
40	62410.0
41	66923.5
42	71437.0
43	73124.0
44	74811.0
45	80732.0
46	86653.0
47	86653.0
48	96311.5
49	105970.0
50	106109.0
51	106248.0
52	108470.0
53	110692.0
54	110692.0
55	104740.5
56	98789.0
57	101136.5
58	103484.0
59	107968.5
60	112453.0
61	112453.0
62	104695.0
63	96937.0
64	90168.5
65	83400.0
66	71603.5
67	59807.0
68	59807.0
69	55431.0
70	51055.0
71	41856.0
72	32657.0
73	21321.5
74	9986.0
75	9986.0
76	7191.0
77	4396.0
78	3531.5
79	2667.0
80	2237.0
81	1807.0
82	1807.0
83	1329.5
84	852.0
85	771.5
86	691.0
87	621.0
88	551.0
89	551.0
90	490.0
91	429.0
92	249.0
93	69.0
94	47.5
95	26.0
96	26.0
97	15.5
98	5.0
99	3.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1558316.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.37614928986514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89800740304155	35.01189755190257
2	12.48094653873004	11.077126937630197
3	3.7099704553423902	4.939026083617887
4	1.525470675996821	2.7077805782138573
5	0.7750415943635656	1.7196680748666342
6	0.46181528791969007	1.2296150496639733
7	0.31878191429469754	0.9902419673755346
8	0.23217475332695264	0.8242417211979571
9	0.17129740024972254	0.6841367105802723
>10	1.1499779954685592	10.151397913587935
>50	0.12422558864562672	3.8600824791723873
>100	0.1203210684665286	11.230758734100554
>500	0.021070690919649828	6.520103633712942
>1k	0.01060800301472026	8.262559575623312
>5k	2.9063021958137695E-4	0.7913629887540818
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6208	0.3983787627156494	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6064	0.3891380182196679	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	4873	0.31270936061748705	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	4584	0.29416369978874635	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4246	0.27247361895790073	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	3574	0.2293501446433201	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	3555	0.22813087974454474	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	3358	0.21548902789934776	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3039	0.19501821196727748	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	2714	0.17416236501454133	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	2657	0.1705045703182153	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	2565	0.16460076133467152	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	2479	0.15908198337179366	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	2434	0.15619425071679943	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	2377	0.1525364560204734	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	2290	0.14695350622081785	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	2256	0.14477166377037778	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	2142	0.1374560743777257	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	2080	0.1334774204975114	No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC	2028	0.13014048498507363	No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA	1924	0.12346661396019805	No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA	1912	0.12269655191886625	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	1908	0.12243986457175567	No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC	1875	0.12032219395809321	No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC	1830	0.11743446130309898	No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA	1829	0.11737028946632133	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	1814	0.1164077119146566	No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT	1724	0.11063224660466811	No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT	1686	0.10819371680711742	No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG	1676	0.10755199843934093	No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA	1665	0.10684610823478678	No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA	1649	0.10581935884634439	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	1618	0.10383003190623724	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	1589	0.1019690486396854	No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG	1575	0.10107064292479832	No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG	1559	0.10004389353635591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	6.417183677764971E-5	0.0	0.0	6.417183677764971E-5	0.0
6	6.417183677764971E-5	0.0	0.0	6.417183677764971E-5	0.0
7	6.417183677764971E-5	0.0	0.0	6.417183677764971E-5	0.0
8	6.417183677764971E-5	0.0	0.0	6.417183677764971E-5	0.0
9	6.417183677764971E-5	0.0	0.0	6.417183677764971E-5	0.0
10	1.2834367355529943E-4	0.0	0.0	6.417183677764971E-5	0.0
11	1.9251551033294917E-4	0.0	0.0	6.417183677764971E-5	0.0
12	1.9251551033294917E-4	0.0	0.0	1.9251551033294917E-4	0.0
13	2.5668734711059885E-4	0.0	0.0	1.9251551033294917E-4	0.0
14	2.5668734711059885E-4	0.0	0.0	2.5668734711059885E-4	0.0
15	3.208591838882486E-4	0.0	0.0	3.8503102066589833E-4	0.0
16	3.8503102066589833E-4	0.0	0.0	7.700620413317967E-4	0.0
17	3.8503102066589833E-4	0.0	0.0	0.001155093061997695	0.0
18	3.8503102066589833E-4	0.0	0.0	0.0014117804091082937	0.0
19	3.8503102066589833E-4	0.0	0.0	0.001604295919441243	0.0
20	3.8503102066589833E-4	0.0	0.0	0.0019251551033294917	0.0
21	3.8503102066589833E-4	0.0	0.0	0.00237435796077304	0.0
22	3.8503102066589833E-4	0.0	0.0	0.0033369355124377854	0.0
23	3.8503102066589833E-4	0.0	0.0	0.004363684900880181	0.0
24	4.49202857443548E-4	0.0	0.0	0.007508104902985017	0.0
25	4.49202857443548E-4	0.0	0.0	0.010203322047646306	0.0
26	5.133746942211977E-4	0.0	0.0	0.015208725316302983	0.0
27	5.133746942211977E-4	0.0	0.0	0.03651377512648269	0.0
28	5.775465309988475E-4	0.0	0.0	0.08849296291637897	0.0
29	5.775465309988475E-4	0.0	0.0	0.1687077588884411	0.0
30	5.775465309988475E-4	0.0	0.0	0.26066600099081316	0.0
31	5.775465309988475E-4	0.0	0.0	0.5294818252523878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2085	0.0	20.496403	1
GGCTACG	90	2.1532178E-6	18.5	1
GCTTTAT	1135	0.0	18.255507	1
CTTATAC	1030	0.0	16.165049	37
ATTACAC	150	4.6729838E-9	16.033333	3
CTACGGC	140	3.475361E-8	15.857143	30
CGAGCGT	95	7.062255E-5	15.578948	6
TACGGCT	120	1.936478E-6	15.416666	31
TTATTGA	1170	0.0	15.337607	4
AAGACGG	305	0.0	15.163935	5
ACGAGCG	110	1.452449E-5	15.136364	5
CTATCCA	125	2.960198E-6	14.799999	9
ACGCTCT	100	1.09376066E-4	14.799999	33
CGAGCCG	200	6.184564E-11	14.799999	15
CTTTATT	1350	0.0	14.799999	2
TTTATTG	1275	0.0	14.509804	3
GCTACGA	115	2.210732E-5	14.47826	2
CGTCAAC	90	8.276346E-4	14.388888	3
CCGGAAC	285	0.0	14.280703	13
GACGGAC	300	0.0	14.183333	7
>>END_MODULE