FastQCFastQC Report
Fri 10 Feb 2017
ERR1630357.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630357.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1897165
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56250.296495033378752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39050.20583344095004916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36770.1938155089304304No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG19510.10283765513279025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17500.019.241
TCTAGCG2050.018.95122128
GTTCGCG609.2398364E-418.530
CTAGCGG2100.018.529
GACGGAC2550.018.1372577
TAGGACA7250.017.3517254
CGCAATA2400.016.95833236
TATACTG2450.016.6122445
TACCGTC2250.016.4444457
ACCGTCG2200.015.9772738
CTTATAC8700.015.94827537
CGCGCTT700.002593326815.85714312
CTTAGGA7000.015.8571432
CGCAAGA3150.015.8571422
CGGACCA2950.015.6779669
GCTTAGG7100.015.6338031
CAAGACG3350.015.4626874
AAGACGG3000.015.4166675
TTAGGAC7700.015.3766233
TCTTATA15000.015.16999937
Produced by FastQC (version 0.11.5)