##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630357.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1897165
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.667845443069	31.0	31.0	34.0	30.0	34.0
2	31.893273384233844	33.0	31.0	34.0	30.0	34.0
3	32.02162331689653	33.0	31.0	34.0	30.0	34.0
4	35.70211710631389	37.0	35.0	37.0	33.0	37.0
5	35.61690891409023	37.0	35.0	37.0	33.0	37.0
6	35.671238400455415	37.0	35.0	37.0	33.0	37.0
7	35.65289207844336	37.0	35.0	37.0	33.0	37.0
8	35.64894513655902	37.0	35.0	37.0	33.0	37.0
9	37.37199031186007	39.0	37.0	39.0	34.0	39.0
10	37.23983364651994	39.0	37.0	39.0	34.0	39.0
11	37.31775781231469	39.0	37.0	39.0	34.0	39.0
12	37.23062253414964	39.0	37.0	39.0	34.0	39.0
13	37.24035073385815	39.0	37.0	39.0	34.0	39.0
14	38.438802107354924	40.0	38.0	41.0	34.0	41.0
15	38.44719146726827	40.0	38.0	41.0	34.0	41.0
16	38.400575068589184	40.0	38.0	41.0	34.0	41.0
17	38.367921082246404	40.0	38.0	41.0	34.0	41.0
18	38.31966275996026	40.0	38.0	41.0	33.0	41.0
19	38.35392809797777	40.0	38.0	41.0	34.0	41.0
20	38.32899352454847	40.0	38.0	41.0	34.0	41.0
21	38.270935316643516	40.0	38.0	41.0	34.0	41.0
22	38.24421123096831	40.0	38.0	41.0	34.0	41.0
23	38.18569286277155	40.0	38.0	41.0	33.0	41.0
24	38.14461841748082	40.0	38.0	41.0	33.0	41.0
25	38.11929800518141	40.0	38.0	41.0	33.0	41.0
26	37.94563994170249	40.0	37.0	41.0	33.0	41.0
27	37.833546370505466	40.0	37.0	41.0	33.0	41.0
28	37.76183621350805	40.0	37.0	41.0	32.0	41.0
29	37.710525969011655	40.0	37.0	41.0	32.0	41.0
30	37.64145501313802	40.0	37.0	41.0	32.0	41.0
31	37.57989737318578	40.0	37.0	41.0	32.0	41.0
32	37.49657568002783	40.0	37.0	41.0	32.0	41.0
33	37.40497268292426	40.0	37.0	41.0	32.0	41.0
34	37.344601023105525	39.0	36.0	41.0	31.0	41.0
35	37.21594537111954	39.0	36.0	41.0	31.0	41.0
36	37.13007830104392	39.0	36.0	41.0	31.0	41.0
37	37.07725000197664	39.0	36.0	41.0	31.0	41.0
38	36.97109792769738	39.0	36.0	41.0	31.0	41.0
39	36.8936550062857	39.0	36.0	41.0	30.0	41.0
40	36.804340687288665	39.0	35.0	40.0	30.0	41.0
41	36.69284326877209	39.0	35.0	40.0	30.0	41.0
42	36.658770850189626	39.0	35.0	40.0	30.0	41.0
43	35.408037782691544	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	2.0
10	2.0
11	5.0
12	3.0
13	1.0
14	2.0
15	3.0
16	27.0
17	65.0
18	106.0
19	208.0
20	382.0
21	736.0
22	1277.0
23	2194.0
24	3599.0
25	5791.0
26	8688.0
27	12407.0
28	17609.0
29	24048.0
30	32317.0
31	42262.0
32	54148.0
33	68699.0
34	90689.0
35	120416.0
36	168379.0
37	256545.0
38	472215.0
39	514338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.56971059449231	19.237072157666834	12.950323245474168	26.24289400236669
2	17.364804853557807	21.247334839088854	35.81586208895905	25.571998218394288
3	18.891293060962013	23.009543186807687	30.985813042091753	27.11335071013855
4	14.430690003241677	15.8891293060962	35.14902499255468	34.53115569810744
5	14.261595591316517	36.97427477314836	34.53821886868037	14.225910766854755
6	34.00927172913268	36.01842749576342	15.974625296165595	13.997675478938309
7	29.14332701689099	31.476966948051434	21.193675826825817	18.186030208231756
8	27.14249946630894	35.07148824693688	19.92504605556185	17.860966231192332
9	26.90551428051856	14.807568134558672	19.26226764672551	39.02464993819726
10	16.416126167202112	27.49739743248479	32.96961518897935	23.116861211333752
11	35.552205527721625	22.03240097724763	21.868208616540997	20.547184878489748
12	21.296249930817826	25.51275192194669	29.796459453974744	23.39453869326073
13	29.69905095234205	19.521760099938593	25.705302385401374	25.073886562317988
14	22.621332356437104	20.30187147665069	25.464996455237156	31.611799711675054
15	24.72974148268601	27.795315642023755	22.48718482577952	24.987758049510717
16	24.70486225499627	26.96655272472347	23.80678538767055	24.52179963260971
17	23.651237504381537	26.909098576033188	25.6089480883318	23.830715831253478
18	23.444507989552832	25.810248449660417	26.692564958767424	24.052678602019327
19	24.422493562763385	25.800128085854418	26.76208974970548	23.015288601676712
20	24.776337324376108	25.197650178028795	26.308939918246438	23.71707257934866
21	24.6157292591841	25.739564033702923	25.401744181449686	24.24296252566329
22	24.643138577825336	25.525191535791564	25.62159854308929	24.21007134329381
23	23.854751695292713	25.652486736788838	26.004485640416092	24.48827592750235
24	24.39924835214649	25.76091167610619	25.940864395031532	23.898975576715785
25	24.60033787256248	25.098502238866942	26.05250465826641	24.248655230304163
26	24.321184504247125	25.849675700321267	25.937069258604282	23.89207053682732
27	24.521167109871836	25.521870791417722	25.523662939175036	24.433299159535412
28	23.818697899233857	25.86237886530692	26.230823360118915	24.088099875340312
29	23.817011171932858	26.038799998945795	26.17937817743844	23.964810651682907
30	23.564845440433487	26.73062174349622	25.950035974730717	23.754496841339577
31	24.26995016247928	25.895902570414275	25.79538416532036	24.03876310178609
32	23.541705650272906	25.876505206452787	26.125613744719097	24.456175398555214
33	23.682547379906335	25.629926759137977	26.37466957275725	24.312856288198443
34	24.268158014721966	25.38809223235723	26.095358073757424	24.248391679163383
35	24.26578605445494	25.556185149947417	26.46475135267623	23.713277442921413
36	23.777689341728315	25.74852477248948	26.270250610779765	24.203535275002437
37	24.485113313812978	24.685728442175563	26.331868867494396	24.497289376517067
38	23.800196609151023	25.0435254709	27.028698083719654	24.12757983622932
39	23.820700887903794	24.83115596165858	26.960016656432096	24.38812649400553
40	23.480878046980628	24.721149715496544	27.61177862758379	24.186193609939043
41	22.87186407086363	24.818821768270023	28.016329628682797	24.29298453218355
42	23.499432047291616	24.46149913159899	27.772702954144734	24.266365866964655
43	22.930899526398598	24.22962683793977	28.104671971072626	24.734801664589003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	125.0
1	129.0
2	133.0
3	369.5
4	606.0
5	606.0
6	829.0
7	1052.0
8	1036.0
9	1020.0
10	1469.0
11	1918.0
12	1918.0
13	3324.5
14	4731.0
15	8661.5
16	12592.0
17	12251.5
18	11911.0
19	11911.0
20	14066.5
21	16222.0
22	17837.0
23	19452.0
24	22504.5
25	25557.0
26	25557.0
27	29753.5
28	33950.0
29	41190.0
30	48430.0
31	53196.5
32	57963.0
33	57963.0
34	64032.0
35	70101.0
36	77316.5
37	84532.0
38	93255.0
39	101978.0
40	101978.0
41	110332.5
42	118687.0
43	115634.0
44	112581.0
45	120048.0
46	127515.0
47	127515.0
48	130397.0
49	133279.0
50	138260.5
51	143242.0
52	150354.0
53	157466.0
54	157466.0
55	143517.0
56	129568.0
57	122558.5
58	115549.0
59	104155.0
60	92761.0
61	92761.0
62	85986.0
63	79211.0
64	70184.0
65	61157.0
66	53290.5
67	45424.0
68	45424.0
69	38763.0
70	32102.0
71	27977.5
72	23853.0
73	19029.5
74	14206.0
75	14206.0
76	10865.0
77	7524.0
78	6351.5
79	5179.0
80	3853.5
81	2528.0
82	2528.0
83	1863.5
84	1199.0
85	997.5
86	796.0
87	685.5
88	575.0
89	575.0
90	485.0
91	395.0
92	230.5
93	66.0
94	45.5
95	25.0
96	25.0
97	14.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1897165.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.00411895825099
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.1811644290954	38.802032009362236
2	11.783818998238853	11.549113359443895
3	3.6593452547314187	5.379689705165091
4	1.5917805653756727	3.1201521672440595
5	0.8776786772333612	2.1504935153132
6	0.5353560208963714	1.5740790079813014
7	0.35734558596264693	1.2257983922603621
8	0.2494259524244445	0.9778319235106026
9	0.18890304103114747	0.8331324384839147
>10	1.2831003759337765	12.233728449798962
>50	0.1516572042958289	5.186862092968549
>100	0.12933278958001215	12.291530359486675
>500	0.00915285380669561	2.9547938762232184
>1k	0.001830570761339122	1.4239430093200147
>5k	1.0768063301994836E-4	0.29681969343802417
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5625	0.296495033378752	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3905	0.20583344095004916	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3677	0.1938155089304304	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1951	0.10283765513279025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.2710228156222575E-5	0.0
3	0.0	0.0	0.0	5.2710228156222575E-5	0.0
4	0.0	0.0	0.0	5.2710228156222575E-5	0.0
5	0.0	0.0	0.0	5.2710228156222575E-5	0.0
6	0.0	0.0	0.0	1.0542045631244515E-4	0.0
7	0.0	0.0	0.0	1.0542045631244515E-4	0.0
8	0.0	0.0	0.0	1.5813068446866773E-4	0.0
9	0.0	0.0	0.0	2.108409126248903E-4	0.0
10	0.0	0.0	0.0	3.1626136893733546E-4	0.0
11	0.0	0.0	0.0	3.1626136893733546E-4	0.0
12	0.0	0.0	0.0	3.6897159709355803E-4	0.0
13	0.0	0.0	0.0	3.6897159709355803E-4	0.0
14	0.0	0.0	0.0	3.6897159709355803E-4	0.0
15	0.0	0.0	0.0	5.271022815622258E-4	0.0
16	0.0	0.0	0.0	5.271022815622258E-4	0.0
17	0.0	0.0	0.0	5.271022815622258E-4	0.0
18	0.0	0.0	0.0	5.798125097184483E-4	0.0
19	0.0	0.0	0.0	5.798125097184483E-4	0.0
20	0.0	0.0	0.0	5.798125097184483E-4	0.0
21	0.0	0.0	0.0	7.379431941871161E-4	0.0
22	0.0	0.0	0.0	0.001106914791280674	0.0
23	0.0	0.0	0.0	0.0015813068446866771	0.0
24	0.0	0.0	0.0	0.0020556988980926804	0.0
25	0.0	0.0	0.0	0.0024246704951862382	0.0
26	0.0	0.0	0.0	0.003584295514623135	0.0
27	0.0	0.0	0.0	0.00901344901471406	0.0
28	0.0	0.0	0.0	0.031362585752952435	0.0
29	0.0	0.0	0.0	0.06652030793315289	0.0
30	0.0	0.0	0.0	0.11870343380781324	0.0
31	0.0	0.0	0.0	0.3338665851415138	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1750	0.0	19.24	1
TCTAGCG	205	0.0	18.951221	28
GTTCGCG	60	9.2398364E-4	18.5	30
CTAGCGG	210	0.0	18.5	29
GACGGAC	255	0.0	18.137257	7
TAGGACA	725	0.0	17.351725	4
CGCAATA	240	0.0	16.958332	36
TATACTG	245	0.0	16.612244	5
TACCGTC	225	0.0	16.444445	7
ACCGTCG	220	0.0	15.977273	8
CTTATAC	870	0.0	15.948275	37
CGCGCTT	70	0.0025933268	15.857143	12
CTTAGGA	700	0.0	15.857143	2
CGCAAGA	315	0.0	15.857142	2
CGGACCA	295	0.0	15.677966	9
GCTTAGG	710	0.0	15.633803	1
CAAGACG	335	0.0	15.462687	4
AAGACGG	300	0.0	15.416667	5
TTAGGAC	770	0.0	15.376623	3
TCTTATA	1500	0.0	15.169999	37
>>END_MODULE