FastQCFastQC Report
Fri 10 Feb 2017
ERR1630356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2768378
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97090.35071077721322735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90610.327303569093527No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77770.28092261967115767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46050.16634289103583397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28050.023.6773621
CTAACGT555.14561E-420.1818184
GCGTACG500.00703732318.521
GACGGAC4900.017.3673467
AAGACGG5050.017.2178235
GTACTAG1401.873559E-917.1785721
GACCGAC650.001580694617.0769237
CGCAAGA5350.016.5981312
ACGGACC5250.016.2095248
CGGACCA5200.016.0096159
GCGCAAG5400.015.7592581
CTTATAC18400.015.68478337
CGGCCTT5550.015.33333324
CAAGACG6450.015.201554
CGTACGG750.00410634814.822
CGAATTA1406.003156E-714.53571415
CTAGATA4100.014.4390253
GTATCAA46600.014.3315452
ATCTCGC6100.014.25409911
TCTACGG1304.4491226E-614.2307692
Produced by FastQC (version 0.11.5)