##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630356.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2768378
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.679631899978975	31.0	31.0	34.0	30.0	34.0
2	31.90021449382996	33.0	31.0	34.0	30.0	34.0
3	32.02341334889961	33.0	31.0	34.0	30.0	34.0
4	35.69699585822456	37.0	35.0	37.0	33.0	37.0
5	35.612867173485704	37.0	35.0	37.0	33.0	37.0
6	35.673794908065304	37.0	35.0	37.0	33.0	37.0
7	35.66334113332789	37.0	35.0	37.0	33.0	37.0
8	35.66665029125358	37.0	35.0	37.0	33.0	37.0
9	37.38119758212209	39.0	37.0	39.0	34.0	39.0
10	37.25603584481599	39.0	37.0	39.0	34.0	39.0
11	37.329882335432515	39.0	37.0	39.0	34.0	39.0
12	37.25232247908342	39.0	37.0	39.0	34.0	39.0
13	37.25992006871894	39.0	37.0	39.0	34.0	39.0
14	38.45238114159266	40.0	38.0	41.0	34.0	41.0
15	38.45841030379522	40.0	38.0	41.0	34.0	41.0
16	38.412532537102955	40.0	38.0	41.0	34.0	41.0
17	38.39589355210885	40.0	38.0	41.0	34.0	41.0
18	38.3519053395165	40.0	38.0	41.0	34.0	41.0
19	38.39926520150066	40.0	38.0	41.0	34.0	41.0
20	38.37667110488524	40.0	38.0	41.0	34.0	41.0
21	38.32094497211002	40.0	38.0	41.0	34.0	41.0
22	38.28236136828136	40.0	38.0	41.0	34.0	41.0
23	38.222219653529976	40.0	38.0	41.0	34.0	41.0
24	38.18356416645415	40.0	38.0	41.0	33.0	41.0
25	38.16334257821728	40.0	38.0	41.0	33.0	41.0
26	37.99714128634168	40.0	37.0	41.0	33.0	41.0
27	37.87863651567813	40.0	37.0	41.0	33.0	41.0
28	37.81457084256557	40.0	37.0	41.0	33.0	41.0
29	37.76110993513169	40.0	37.0	41.0	33.0	41.0
30	37.69282735233411	40.0	37.0	41.0	33.0	41.0
31	37.6286153841708	40.0	37.0	41.0	32.0	41.0
32	37.53452852175534	40.0	37.0	41.0	32.0	41.0
33	37.445604610353065	40.0	37.0	41.0	32.0	41.0
34	37.38673367582028	40.0	36.0	41.0	32.0	41.0
35	37.26311941505098	39.0	36.0	41.0	31.0	41.0
36	37.17446714285405	39.0	36.0	41.0	31.0	41.0
37	37.1235626059736	39.0	36.0	41.0	31.0	41.0
38	37.01481950802961	39.0	36.0	41.0	31.0	41.0
39	36.93891441125453	39.0	36.0	41.0	31.0	41.0
40	36.84005363429416	39.0	36.0	41.0	30.0	41.0
41	36.72582970967115	39.0	35.0	41.0	30.0	41.0
42	36.688027429780185	39.0	35.0	40.0	30.0	41.0
43	35.501541696979245	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	6.0
10	9.0
11	9.0
12	6.0
13	6.0
14	4.0
15	8.0
16	33.0
17	74.0
18	132.0
19	240.0
20	532.0
21	998.0
22	1774.0
23	3085.0
24	4976.0
25	7901.0
26	11989.0
27	17551.0
28	24819.0
29	34422.0
30	46343.0
31	60537.0
32	77272.0
33	100046.0
34	131087.0
35	175220.0
36	246332.0
37	373284.0
38	688897.0
39	760781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.29486652473037	20.58660341904176	13.320724265255684	24.797805790972184
2	17.401814347607157	22.170455046240072	36.38610767749202	24.041622928660754
3	19.2704175513604	24.533065932470205	30.793013092865205	25.403503423304187
4	14.249318554041393	16.442480037046963	35.951087604366165	33.35711380454548
5	13.559708970379045	37.84996124084211	34.764977904029	13.825351884749843
6	32.37498636385638	36.75950321812989	16.702415638326848	14.163094779686878
7	27.549633756661844	31.9333559217708	22.437904072348502	18.079106249218857
8	25.953428325178134	35.23261635513648	20.96671769534363	17.847237624341762
9	26.316781884554786	15.050690332028358	20.80116949347235	37.83135828994451
10	16.381397338080276	27.59749571770907	33.95186639974743	22.06924054446322
11	34.06883019587643	22.980134938220143	22.544067320286466	20.406967545616965
12	20.45273441704854	26.826574983618567	30.755373724252976	21.96531687507992
13	28.95233237657574	20.870740917605904	26.444148884292535	23.73277782152582
14	22.020836749894706	21.849003279176472	26.587192933912927	29.5429670370159
15	24.13106158190825	28.714286849555947	23.825648087074814	23.329003481460987
16	23.673717967705276	27.6605290173524	25.16105098364457	23.50470203129775
17	22.355111910295484	27.801947566408924	26.69552351593605	23.147417007359543
18	22.08231679344367	26.39014614333736	28.348188000338105	23.179349062880863
19	23.02348161992329	26.59553717014078	28.371233986110276	22.00974722382565
20	23.632899842434814	25.9329470180734	28.092298089350514	22.341855050141273
21	23.647926692091904	26.57610340784387	26.99067829609974	22.785291603964488
22	23.32412697976938	26.31165252721991	27.489381869094466	22.874838623916244
23	22.755707493702086	26.49497286858948	27.628091250544546	23.121228387163892
24	23.271280150326294	26.6907553809487	27.545840922012815	22.49212354671219
25	23.268318127076576	26.183599205021856	27.57340218712907	22.9746804807725
26	22.952176328521613	26.76794859661506	27.485372301036925	22.794502773826405
27	23.223201455870548	26.295505888285486	27.16233115564421	23.318961500199755
28	22.598756383701936	26.644338309291577	27.85241032835834	22.904494978648145
29	22.5968780274948	26.81393220145515	27.894492731845144	22.694697039204907
30	22.388669466380676	27.461820603978214	27.57369116500709	22.575818764634022
31	22.989924063838103	26.83683369828831	27.553715569188892	22.619526668684696
32	22.269429969462262	26.737100208136315	27.589657192767753	23.40381262963367
33	22.360963712325415	26.44888089704513	28.07571798359906	23.114437407030398
34	22.87176823396227	26.028598695698346	27.973817159361907	23.125815910977472
35	22.948094515994562	26.221780407155382	28.064772946469013	22.76535213038104
36	22.504441228762836	26.65701721368975	27.782875026459536	23.05566653108788
37	23.17931294064611	25.735394516211297	27.720455804807003	23.36483673833559
38	22.581417711020677	25.967190896618884	28.462623240034418	22.98876815232602
39	22.60980978753624	25.570785492443587	28.569834032780207	23.249570687239967
40	22.19339266530799	25.423551263591893	29.234013563176703	23.14904250792341
41	21.637507594699855	25.715888509444877	29.513021704405972	23.13358219144929
42	22.195126532576115	25.33848340074946	29.219275691397634	23.247114375276787
43	21.583107509162407	25.129733006114048	29.38038085839434	23.90677862632921
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1694.0
1	1363.5
2	1033.0
3	1840.0
4	2647.0
5	2647.0
6	3303.0
7	3959.0
8	3586.0
9	3213.0
10	4895.0
11	6577.0
12	6577.0
13	10392.0
14	14207.0
15	20095.5
16	25984.0
17	26125.0
18	26266.0
19	26266.0
20	29971.5
21	33677.0
22	36047.5
23	38418.0
24	44012.0
25	49606.0
26	49606.0
27	56062.0
28	62518.0
29	77457.0
30	92396.0
31	99067.0
32	105738.0
33	105738.0
34	114067.5
35	122397.0
36	131428.0
37	140459.0
38	151286.5
39	162114.0
40	162114.0
41	171584.0
42	181054.0
43	175263.0
44	169472.0
45	175576.5
46	181681.0
47	181681.0
48	183740.0
49	185799.0
50	187423.5
51	189048.0
52	194911.0
53	200774.0
54	200774.0
55	186500.5
56	172227.0
57	161828.0
58	151429.0
59	136804.5
60	122180.0
61	122180.0
62	111101.0
63	100022.0
64	86960.0
65	73898.0
66	62938.0
67	51978.0
68	51978.0
69	43778.0
70	35578.0
71	30889.0
72	26200.0
73	20564.0
74	14928.0
75	14928.0
76	11383.0
77	7838.0
78	6331.0
79	4824.0
80	3718.5
81	2613.0
82	2613.0
83	1962.0
84	1311.0
85	1181.0
86	1051.0
87	946.0
88	841.0
89	841.0
90	702.5
91	564.0
92	332.0
93	100.0
94	76.5
95	53.0
96	53.0
97	30.5
98	8.0
99	6.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2768378.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.50456947189265
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.97856006639894	46.056892518830566
2	10.38281347417517	11.525871835821176
3	2.8570947667350954	4.757454449040871
4	1.160734441560821	2.577042618001244
5	0.6350522906409045	1.762415199208132
6	0.3898331295991639	1.2982512014569272
7	0.25999032732271665	1.0101455829432684
8	0.1846632106664604	0.819972160427144
9	0.14215171903556387	0.7101062968282577
>10	0.8163094646922	8.60708047900807
>50	0.08828273311673648	3.477287889284471
>100	0.09264060753029425	10.822059632355534
>500	0.009003187124131156	3.4486337570943424
>1k	0.0026748599426709764	2.1654809873122542
>5k	1.9572145921982752E-4	0.961305392387726
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9709	0.35071077721322735	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9061	0.327303569093527	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7777	0.28092261967115767	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4605	0.16634289103583397	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.22444695052482E-5	0.0
5	0.0	0.0	0.0	7.22444695052482E-5	0.0
6	0.0	0.0	0.0	7.22444695052482E-5	0.0
7	0.0	0.0	0.0	1.083667042578723E-4	0.0
8	0.0	0.0	0.0	1.444889390104964E-4	0.0
9	0.0	0.0	0.0	1.444889390104964E-4	0.0
10	0.0	0.0	0.0	1.444889390104964E-4	0.0
11	0.0	0.0	0.0	2.889778780209928E-4	0.0
12	0.0	0.0	0.0	4.334668170314892E-4	0.0
13	0.0	0.0	0.0	5.057112865367374E-4	0.0
14	0.0	0.0	0.0	6.140779907946096E-4	0.0
15	0.0	0.0	0.0	8.308113993103543E-4	0.0
16	0.0	0.0	0.0	0.0012642782163418435	0.0
17	0.0	0.0	0.0	0.0016616227986207086	0.0
18	0.0	0.0	0.0	0.0019867229113943257	0.0
19	0.0	0.0	0.0	0.002275700789415318	0.0
20	0.0	0.0	0.0	0.0024563119631784386	0.0
21	0.0	0.0	0.0	0.0030703899539730483	0.0
22	0.0	0.0	0.0	0.004226301466057019	0.0
23	0.0	0.0	0.0	0.0063575133164618415	0.0
24	0.0	0.0	0.0	0.009572392209445387	0.0
25	0.0	0.0	0.0	0.011342381712323968	0.0
26	0.0	0.0	0.0	0.014376649431544392	0.0
27	0.0	0.0	0.0	0.030451043896462117	0.0
28	0.0	0.0	0.0	0.07607342638902635	0.0
29	0.0	0.0	0.0	0.1385287702763134	0.0
30	0.0	0.0	0.0	0.22561947826489012	0.0
31	0.0	0.0	0.0	0.4815816337219845	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2805	0.0	23.677362	1
CTAACGT	55	5.14561E-4	20.181818	4
GCGTACG	50	0.007037323	18.5	21
GACGGAC	490	0.0	17.367346	7
AAGACGG	505	0.0	17.217823	5
GTACTAG	140	1.873559E-9	17.178572	1
GACCGAC	65	0.0015806946	17.076923	7
CGCAAGA	535	0.0	16.598131	2
ACGGACC	525	0.0	16.209524	8
CGGACCA	520	0.0	16.009615	9
GCGCAAG	540	0.0	15.759258	1
CTTATAC	1840	0.0	15.684783	37
CGGCCTT	555	0.0	15.333333	24
CAAGACG	645	0.0	15.20155	4
CGTACGG	75	0.004106348	14.8	22
CGAATTA	140	6.003156E-7	14.535714	15
CTAGATA	410	0.0	14.439025	3
GTATCAA	4660	0.0	14.331545	2
ATCTCGC	610	0.0	14.254099	11
TCTACGG	130	4.4491226E-6	14.230769	2
>>END_MODULE