FastQCFastQC Report
Fri 10 Feb 2017
ERR1630355.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630355.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2272380
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112650.4957357484223589No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT80690.35509025779139053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78550.34567281880671363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.18557635606720707No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG28430.12511111697867433No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT25990.11437347626717363No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC25500.11221714678002798No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA25010.11006081729288235No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA24840.10931270298101549No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC23170.10196358003502935No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA22800.10033533123861325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCG303.600576E-430.8333349
TACGACG1601.8189894E-1218.55
CGAATTA1409.458745E-1118.515
CTTATAC15800.017.44620137
CGGCCTT7350.017.36734624
CTAGTAC1352.2224413E-816.4444453
ATCTCGC8000.016.4187511
GACGGTA1751.3278623E-1015.8571428
TCTCGCC8300.015.82530112
TTCCTCG8000.015.72519
CGACGGT1802.0190782E-1015.4166667
ACCGTCG1701.4879333E-915.2352938
GGTATCA39900.015.0689221
TTGGCCG6450.014.62790731
CCGAATT1904.5656634E-1014.60526414
CTCGCCT9100.014.027472513
TCTTATA26750.013.83177637
TATACAG3100.013.7258075
GGCCGCA7050.013.645390533
ACGGTAT2051.4260877E-913.5365869
Produced by FastQC (version 0.11.5)