##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630355.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2272380
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.807015551976342	33.0	31.0	34.0	30.0	34.0
2	32.02363777185154	33.0	31.0	34.0	30.0	34.0
3	32.15100731391757	34.0	31.0	34.0	30.0	34.0
4	35.80070630792385	37.0	35.0	37.0	35.0	37.0
5	35.72899911106417	37.0	35.0	37.0	35.0	37.0
6	35.78982124468619	37.0	35.0	37.0	35.0	37.0
7	35.77424286430966	37.0	35.0	37.0	33.0	37.0
8	35.77496369445251	37.0	35.0	37.0	35.0	37.0
9	37.51865489046726	39.0	37.0	39.0	35.0	39.0
10	37.41561534602487	39.0	37.0	39.0	34.0	39.0
11	37.47484531636434	39.0	37.0	39.0	35.0	39.0
12	37.40981613990618	39.0	37.0	39.0	34.0	39.0
13	37.41722467192987	39.0	37.0	39.0	34.0	39.0
14	38.63792983567889	40.0	38.0	41.0	34.0	41.0
15	38.65353814062789	40.0	38.0	41.0	34.0	41.0
16	38.6153719888399	40.0	38.0	41.0	34.0	41.0
17	38.607211821966395	40.0	38.0	41.0	34.0	41.0
18	38.57193383148945	40.0	38.0	41.0	34.0	41.0
19	38.62637278976228	40.0	38.0	41.0	34.0	41.0
20	38.60822837729605	40.0	38.0	41.0	34.0	41.0
21	38.55548763851117	40.0	38.0	41.0	34.0	41.0
22	38.52645816280728	40.0	38.0	41.0	34.0	41.0
23	38.47389301085206	40.0	38.0	41.0	34.0	41.0
24	38.4435802990697	40.0	38.0	41.0	34.0	41.0
25	38.42464772617256	40.0	38.0	41.0	34.0	41.0
26	38.27490780591275	40.0	38.0	41.0	34.0	41.0
27	38.15881894753518	40.0	38.0	41.0	33.0	41.0
28	38.10520687561059	40.0	38.0	41.0	33.0	41.0
29	38.05275834147458	40.0	38.0	41.0	33.0	41.0
30	37.983383500998954	40.0	37.0	41.0	33.0	41.0
31	37.91868261470353	40.0	37.0	41.0	33.0	41.0
32	37.82627905543967	40.0	37.0	41.0	33.0	41.0
33	37.74262315281775	40.0	37.0	41.0	33.0	41.0
34	37.67963368802753	40.0	37.0	41.0	33.0	41.0
35	37.56732764766456	40.0	37.0	41.0	32.0	41.0
36	37.48068016792966	40.0	37.0	41.0	32.0	41.0
37	37.42326063422491	40.0	37.0	41.0	32.0	41.0
38	37.322611535042554	40.0	36.0	41.0	31.0	41.0
39	37.23980716253443	40.0	36.0	41.0	31.0	41.0
40	37.1470779535113	40.0	36.0	41.0	31.0	41.0
41	37.031255775882556	39.0	36.0	41.0	31.0	41.0
42	36.98979704098786	39.0	36.0	41.0	31.0	41.0
43	35.8355512722344	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	7.0
10	11.0
11	8.0
12	10.0
13	2.0
14	5.0
15	7.0
16	17.0
17	22.0
18	83.0
19	145.0
20	275.0
21	561.0
22	1070.0
23	1866.0
24	3308.0
25	5299.0
26	8116.0
27	12363.0
28	17935.0
29	24981.0
30	34213.0
31	45059.0
32	58366.0
33	75604.0
34	99384.0
35	134135.0
36	186457.0
37	286690.0
38	524536.0
39	751844.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41010746442056	20.27121344141385	13.491141446413014	24.827537647752575
2	16.539443226925073	22.170411638898425	37.401138894023006	23.889006240153495
3	18.702637763050195	24.467650657020393	31.663102122004243	25.16660945792517
4	13.913121925030145	16.349246164813984	36.293005571251285	33.444626338904584
5	13.217067567924378	38.177109462325845	35.03965005852894	13.566172911220834
6	32.141279187459844	37.03623513672889	16.817125656800357	14.005360019010904
7	27.150432586099154	32.35880442531619	22.51225587269735	17.978507115887307
8	25.681004057419976	36.15724482700956	20.8347195451465	17.327031570423962
9	25.969248101109848	15.461850570767213	20.79762187662275	37.77127945150019
10	16.00273721824695	28.429884086288386	33.96095723426539	21.60642146119927
11	33.48418838398508	23.39564685483942	22.912276995924977	20.20788776525053
12	20.117629973860005	27.231272938505	30.92968605602936	21.721411031605626
13	28.730538026210407	20.935142889833568	26.72501958299228	23.609299500963747
14	21.641318793511648	21.90258671525009	26.928682702716976	29.527411788521285
15	23.695156619931527	28.984060764484816	24.25289784279038	23.06788477279328
16	23.167031922477754	28.35353241975374	25.203047025585512	23.276388632183
17	21.948485728619332	28.262922574569394	26.94461313688732	22.843978559923954
18	21.380975012981985	26.966220438483003	28.762662934896454	22.890141613638566
19	22.716711113458135	27.12970541898802	28.886497856872527	21.267085610681313
20	23.245715945396455	26.4608032107306	28.6071431714766	21.68633767239634
21	22.969881797938722	27.265950237196247	27.26964680203135	22.49452116283368
22	22.851459703042625	26.915524692172966	27.66817169663525	22.564843908149165
23	22.23408936885556	27.247995493711443	27.840546035434215	22.677369101998785
24	23.156118254869344	27.165966959751447	27.72608454571859	21.95183023966062
25	22.90585201418777	26.579929413214337	27.933268203381477	22.580950369216417
26	22.68441017787518	26.997817266478318	27.788706114294264	22.52906644135224
27	22.66808368318679	26.78328448586944	27.67574085320237	22.8728909777414
28	22.056170182803932	27.31211329091085	28.169232258689124	22.46248426759609
29	22.29596282311937	27.590719861994913	28.04874184775434	22.064575467131377
30	21.891937087987046	28.203557503586545	27.77748439961626	22.127021008810146
31	22.559123033999594	27.395462026597666	27.974722537603746	22.070692401798993
32	21.660153671481	27.478062647972614	28.05780723294519	22.803976447601194
33	21.99258046629525	27.077337417157345	28.354280534065605	22.5758015824818
34	22.147132081782097	26.855895580844752	28.300416303611193	22.696556033761958
35	22.385692533819167	26.956142898634912	28.138427551729905	22.519737015816016
36	22.026465643950395	27.106601888768605	28.210378545841802	22.656553921439198
37	22.65734604247529	25.959566621779807	28.151541555549684	23.231545780195216
38	22.015860023411577	26.470924757302917	28.93882185197898	22.574393367306524
39	21.90971580457494	26.030989535200977	29.33787482727361	22.721419832950474
40	21.681936999973598	25.680475976729245	29.854469762979786	22.783117260317375
41	21.044895660056856	26.326978762354887	29.99595138137107	22.632174196217182
42	21.467052165570898	25.664281502213537	29.754222445189626	23.11444388702594
43	20.765761008282066	25.679947896038513	29.870576224047035	23.683714871632386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	747.0
1	644.0
2	541.0
3	1307.0
4	2073.0
5	2073.0
6	2744.0
7	3415.0
8	3288.5
9	3162.0
10	4559.0
11	5956.0
12	5956.0
13	9578.5
14	13201.0
15	20928.0
16	28655.0
17	27156.0
18	25657.0
19	25657.0
20	28186.5
21	30716.0
22	32690.5
23	34665.0
24	39417.5
25	44170.0
26	44170.0
27	49268.0
28	54366.0
29	67446.0
30	80526.0
31	84897.5
32	89269.0
33	89269.0
34	96295.5
35	103322.0
36	109323.0
37	115324.0
38	126201.0
39	137078.0
40	137078.0
41	145932.0
42	154786.0
43	146955.0
44	139124.0
45	145656.0
46	152188.0
47	152188.0
48	153496.0
49	154804.0
50	157740.5
51	160677.0
52	164535.5
53	168394.0
54	168394.0
55	149990.5
56	131587.0
57	122508.5
58	113430.0
59	101399.5
60	89369.0
61	89369.0
62	81368.0
63	73367.0
64	64055.5
65	54744.0
66	46533.5
67	38323.0
68	38323.0
69	32064.0
70	25805.0
71	22105.0
72	18405.0
73	14685.5
74	10966.0
75	10966.0
76	8378.5
77	5791.0
78	4745.0
79	3699.0
80	2801.5
81	1904.0
82	1904.0
83	1378.5
84	853.0
85	708.0
86	563.0
87	483.0
88	403.0
89	403.0
90	336.5
91	270.0
92	166.5
93	63.0
94	38.0
95	13.0
96	13.0
97	9.5
98	6.0
99	4.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2272380.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.42256304599885
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.17172921786667	44.17573550912096
2	11.683743647831069	12.7171855057475
3	3.2618166699227262	5.325492700900788
4	1.310084766149307	2.8519268312545325
5	0.656307866093791	1.7858978110037154
6	0.38244885723975025	1.2488308227000313
7	0.24350717786592044	0.9276599317692917
8	0.17050793419324067	0.7423583038779732
9	0.13013014154001312	0.6373814248931506
>10	0.8045735766406175	8.311467724906816
>50	0.08479787312519194	3.23690957908575
>100	0.08683020816139307	10.032283356289728
>500	0.009635482281780131	3.589499508490472
>1k	0.0036436697704210373	3.2192553935545174
>5k	1.6194087868537943E-4	0.7017099840799689
>10k+	8.097043934268972E-5	0.49640561232484615
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11265	0.4957357484223589	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8069	0.35509025779139053	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7855	0.34567281880671363	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4217	0.18557635606720707	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2843	0.12511111697867433	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2599	0.11437347626717363	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2550	0.11221714678002798	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2501	0.11006081729288235	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	2484	0.10931270298101549	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	2317	0.10196358003502935	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	2280	0.10033533123861325	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	4.4006724227461956E-5	0.0
3	0.0	0.0	0.0	4.4006724227461956E-5	0.0
4	0.0	0.0	0.0	4.4006724227461956E-5	0.0
5	0.0	0.0	0.0	4.4006724227461956E-5	0.0
6	0.0	0.0	0.0	4.4006724227461956E-5	0.0
7	0.0	0.0	0.0	4.4006724227461956E-5	0.0
8	0.0	0.0	0.0	8.801344845492391E-5	0.0
9	0.0	0.0	0.0	1.3202017268238586E-4	0.0
10	0.0	0.0	0.0	3.960605180471576E-4	0.0
11	0.0	0.0	0.0	4.840739665020815E-4	0.0
12	0.0	0.0	0.0	5.720874149570055E-4	0.0
13	0.0	0.0	0.0	6.160941391844674E-4	0.0
14	0.0	0.0	0.0	8.361277603217772E-4	0.0
15	0.0	0.0	0.0	9.68147933004163E-4	0.0
16	0.0	0.0	0.0	0.0014082151752787826	0.0
17	0.0	0.0	0.0	0.0018042756933259402	0.0
18	0.0	0.0	0.0	0.00202430931446325	0.0
19	0.0	0.0	0.0	0.0024643765567378697	0.0
20	0.0	0.0	0.0	0.002816430350557565	0.0
21	0.0	0.0	0.0	0.0035645446624244185	0.0
22	0.0	0.0	0.0	0.005060773286158125	0.0
23	0.0	4.4006724227461956E-5	0.0	0.006997069152166451	0.0
24	0.0	4.4006724227461956E-5	0.0	0.010297573469226098	0.0
25	0.0	4.4006724227461956E-5	0.0	0.011881815541414728	0.0
26	0.0	4.4006724227461956E-5	0.0	0.014786259340427217	0.0
27	0.0	4.4006724227461956E-5	0.0	0.030320632992721287	0.0
28	0.0	4.4006724227461956E-5	0.0	0.08264462809917356	0.0
29	0.0	4.4006724227461956E-5	0.0	0.15719201894049412	0.0
30	0.0	4.4006724227461956E-5	0.0	0.2690571119267024	0.0
31	0.0	4.4006724227461956E-5	0.0	0.6109893591740818	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCGCG	30	3.600576E-4	30.833334	9
TACGACG	160	1.8189894E-12	18.5	5
CGAATTA	140	9.458745E-11	18.5	15
CTTATAC	1580	0.0	17.446201	37
CGGCCTT	735	0.0	17.367346	24
CTAGTAC	135	2.2224413E-8	16.444445	3
ATCTCGC	800	0.0	16.41875	11
GACGGTA	175	1.3278623E-10	15.857142	8
TCTCGCC	830	0.0	15.825301	12
TTCCTCG	800	0.0	15.725	19
CGACGGT	180	2.0190782E-10	15.416666	7
ACCGTCG	170	1.4879333E-9	15.235293	8
GGTATCA	3990	0.0	15.068922	1
TTGGCCG	645	0.0	14.627907	31
CCGAATT	190	4.5656634E-10	14.605264	14
CTCGCCT	910	0.0	14.0274725	13
TCTTATA	2675	0.0	13.831776	37
TATACAG	310	0.0	13.725807	5
GGCCGCA	705	0.0	13.6453905	33
ACGGTAT	205	1.4260877E-9	13.536586	9
>>END_MODULE