FastQCFastQC Report
Fri 10 Feb 2017
ERR1630354.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630354.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1974056
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT38040.19269970051508165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT35310.17887030560429898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27540.13950972008899443No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT609.2400145E-418.54
TACGTTA1103.855712E-818.519
TACCGTC1002.877514E-718.4999987
CGTCGTA1005.8820915E-616.6510
CGCGCTT700.002593377615.85714212
GTCTAAC1101.4528388E-515.1363641
TATACGC1101.4528388E-515.1363647
ATTACGT1508.1097824E-814.817
TAACGCT1406.000628E-714.53571432
ATACGCT1152.2113229E-514.478268
GGTATCA22100.014.39819051
TTTAGAC1803.3342076E-914.3888883
GTAGAAC4000.014.3374993
ACGTTAA1551.2113924E-714.3225820
CGAGCCG2500.014.05999915
CTTAACG1458.918505E-714.03448430
TAGAACA4350.014.0344834
TCTTATA9400.013.97340537
TACGCTA1203.3027165E-513.8750019
ACCGTCG1203.3027165E-513.8750018
Produced by FastQC (version 0.11.5)