FastQCFastQC Report
Fri 10 Feb 2017
ERR1630353.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630353.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1099936
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC66140.6013077124487243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65800.5982166235126407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT48960.4451168067960318No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT47450.43138873534460187No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41010.3728398743199604No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT29530.26847016553690395No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG28550.25956055625054547No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG27800.25274197771506707No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG24430.22210383149565063No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT23300.21183050650219648No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.2098303901317895No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG21960.19964797951880836No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC21680.1971023768655631No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT21400.19455677421231782No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT20690.18810185319873157No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG19970.18155601780467226No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG19570.17791944258575043No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC19020.17291915165973293No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG18770.1706462921479068No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT18440.16764611759229628No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG17940.163100398568644No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT17600.16000930963256044No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG17210.15646364879411168No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT15780.14346289238646612No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT14600.13273499549064674No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA14210.12918933465219795No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA14070.12791653332557532No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC13810.12555275943327612No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC13750.12500727315043783No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC13560.12327989992144998No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA13510.12282532801908475No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT13440.12218892735577343No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG12870.11700680766880982No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA12640.11491577691792977No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA12560.1141884618741454No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA12390.11264291740610362No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC11480.10436970878305647No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG11380.10346056497832601No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC11220.10200593489075728No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG11160.101460448607919No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG250.00549624229.61
TGCATCA14600.019.76712214
GGACAGG15200.019.7171066
ACAGGCT15400.019.1006498
CAGGACA15600.019.0929494
GCATCAG14950.019.05685615
GACAGGC15100.018.9900677
CTGCATC15450.018.67961113
CCAGGAC16550.018.6676753
GCTGCAT15300.018.62091412
AGCGTCA701.2191364E-418.53
ATAGGCG1103.8511644E-818.532
ACCGAGT500.00703469518.58
TTATACG701.2191364E-418.535
ACAATCG500.00703469518.537
CGATAGA902.1520445E-618.537
TCCAGGA16950.018.008852
GGCCATC16550.017.66163326
TGGACCG752.0671637E-417.2666665
TTAGAAC650.001579875217.0769233
Produced by FastQC (version 0.11.5)