##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630353.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1099936
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.722044737148344	31.0	31.0	34.0	30.0	34.0
2	31.94304850464027	33.0	31.0	34.0	30.0	34.0
3	32.03630120297908	33.0	31.0	34.0	30.0	34.0
4	35.71703171820906	37.0	35.0	37.0	33.0	37.0
5	35.66026386989788	37.0	35.0	37.0	33.0	37.0
6	35.71274419602595	37.0	35.0	37.0	33.0	37.0
7	35.695600471300146	37.0	35.0	37.0	33.0	37.0
8	35.692703939138276	37.0	35.0	37.0	33.0	37.0
9	37.43230333401216	39.0	37.0	39.0	34.0	39.0
10	37.32133324120676	39.0	37.0	39.0	34.0	39.0
11	37.38913264044454	39.0	37.0	39.0	34.0	39.0
12	37.32229784278358	39.0	37.0	39.0	34.0	39.0
13	37.30991348587554	39.0	37.0	39.0	34.0	39.0
14	38.51454448258808	40.0	38.0	41.0	34.0	41.0
15	38.51886291566055	40.0	38.0	41.0	34.0	41.0
16	38.47534492915951	40.0	38.0	41.0	34.0	41.0
17	38.4620623381724	40.0	38.0	41.0	34.0	41.0
18	38.33776237890204	40.0	38.0	41.0	33.0	41.0
19	38.39550482937189	40.0	38.0	41.0	34.0	41.0
20	38.37141706426556	40.0	38.0	41.0	34.0	41.0
21	38.330411042097	40.0	38.0	41.0	34.0	41.0
22	38.279371708899426	40.0	38.0	41.0	34.0	41.0
23	38.222875694585866	40.0	38.0	41.0	33.0	41.0
24	38.172047282750995	40.0	38.0	41.0	33.0	41.0
25	38.149875992785034	40.0	38.0	41.0	33.0	41.0
26	37.93115963110581	40.0	37.0	41.0	33.0	41.0
27	37.81086535943909	40.0	37.0	41.0	33.0	41.0
28	37.720738297500944	40.0	37.0	41.0	32.0	41.0
29	37.637808927064846	40.0	37.0	41.0	32.0	41.0
30	37.56412373083525	40.0	37.0	41.0	32.0	41.0
31	37.505968529078054	40.0	37.0	41.0	32.0	41.0
32	37.40605635236959	40.0	36.0	41.0	32.0	41.0
33	37.29668726180432	40.0	36.0	41.0	31.0	41.0
34	37.19906158176476	39.0	36.0	41.0	31.0	41.0
35	37.03050268379251	39.0	36.0	41.0	31.0	41.0
36	36.93587081430192	39.0	35.0	41.0	31.0	41.0
37	36.832873003520206	39.0	35.0	41.0	30.0	41.0
38	36.735110042766124	39.0	35.0	41.0	30.0	41.0
39	36.640263615337616	39.0	35.0	41.0	30.0	41.0
40	36.48067978500567	39.0	35.0	41.0	30.0	41.0
41	36.32107413522241	39.0	35.0	40.0	29.0	41.0
42	36.2792707939371	39.0	35.0	40.0	29.0	41.0
43	35.00380294853519	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	3.0
10	0.0
11	5.0
12	3.0
13	3.0
14	3.0
15	8.0
16	7.0
17	22.0
18	60.0
19	134.0
20	234.0
21	495.0
22	837.0
23	1438.0
24	2271.0
25	3661.0
26	5381.0
27	7791.0
28	10740.0
29	14660.0
30	19601.0
31	24779.0
32	31897.0
33	41643.0
34	55477.0
35	71873.0
36	96060.0
37	144586.0
38	253849.0
39	312413.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.817945771390335	17.644299304686818	12.509727838710615	27.02802708521223
2	18.889462659645652	19.717238093852732	35.11040642364647	26.282892822855146
3	20.41236944694964	22.124650888778984	29.30706877491054	28.155910889360836
4	15.603089634305997	14.85422788234952	32.334335815902016	37.20834666744247
5	15.321527797980975	36.61003912954936	32.40315800192011	15.665275070549558
6	36.283201931748756	32.71308512495273	15.445716841707155	15.557996101591364
7	30.64323742472289	29.563992814127367	20.38336775957874	19.409402001571
8	28.713216041660605	33.25566214761586	18.697996974369417	19.333124836354116
9	27.94862610188229	13.872716230762517	18.5243505076659	39.65430715968929
10	18.804730457044773	26.65691458412126	30.60932636080645	23.92902859802752
11	35.90936199924359	21.762266168213422	20.30936345387368	22.019008378669305
12	23.72510764262648	24.56924766531871	27.707430250487302	23.99821444156751
13	30.651328804584992	18.661085735897363	24.369417857038954	26.318167602478688
14	23.208623047159108	19.743876007331334	25.118370523375905	31.92913042213365
15	26.621821633258662	25.98814840136153	21.59143804730457	25.798591918075232
16	24.824626160067496	25.12064338288773	22.45439734675472	27.600333110290055
17	24.149132313152766	26.212070520466646	25.206466558054284	24.432330608326303
18	23.490821284147444	23.758291391499142	26.341896255782153	26.408991068571265
19	24.957724813080034	23.901754283885605	26.206888400779683	24.933632502254678
20	24.48687923661013	24.8384451458994	26.023786838506968	24.650888778983504
21	27.314407383702328	23.629738457510253	24.696345969220026	24.359508189567393
22	26.47044918977104	24.63934265266343	24.719892793762547	24.170315363802985
23	25.624309050708405	24.925177464870686	24.195771390335437	25.254742094085476
24	25.491483140837286	24.599794897157654	25.12182526983388	24.786896692171183
25	25.145644837517818	23.971031041806068	25.85277688883717	25.030547231838945
26	26.10406423646467	24.266139120821574	25.864868501440085	23.764928141273675
27	26.366806796031767	24.408147383120475	24.447331481104356	24.777714339743405
28	24.73616646786722	23.51764102638699	26.360715532540073	25.385476973205712
29	24.854900648765017	23.67283187385448	26.57036409391092	24.901903383469584
30	24.266139120821574	24.282503709306724	26.18388706252	25.267470107351702
31	24.172133651412445	24.548155529048962	25.945782300061094	25.333928519477496
32	23.731017077357226	24.602431414191372	26.353078724580335	25.31347278387106
33	25.268470165536904	24.9092674482879	25.448753382014957	24.373509004160244
34	25.35374785442062	25.00372748959939	25.474391237308353	24.16813341867163
35	25.539122276205163	24.529518081051986	26.099609431821484	23.831750210921363
36	25.26046970005528	24.80844340034329	24.353871497978062	25.577215401623366
37	23.42354464259739	24.310959910394786	26.264073546097226	26.0014219009106
38	25.262287987664738	23.870843394524773	26.362533820149537	24.504334797660952
39	22.92778852587787	24.436603584208534	27.05548322811509	25.580124661798504
40	24.026397899514155	23.027067029354434	28.3051013877171	24.641433683414306
41	22.490035783900154	23.32963008756873	28.697942425741136	25.48239170278998
42	22.378665667820673	24.283231024350506	27.366410409332907	25.971692898495913
43	22.413940447444215	22.08010284234719	29.275794228027813	26.23016248218078
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	562.0
1	400.5
2	239.0
3	521.5
4	804.0
5	804.0
6	1130.0
7	1456.0
8	1364.0
9	1272.0
10	1714.0
11	2156.0
12	2156.0
13	4128.0
14	6100.0
15	9893.0
16	13686.0
17	11716.0
18	9746.0
19	9746.0
20	10821.0
21	11896.0
22	9811.5
23	7727.0
24	9035.0
25	10343.0
26	10343.0
27	12369.5
28	14396.0
29	17470.0
30	20544.0
31	23934.5
32	27325.0
33	27325.0
34	31779.5
35	36234.0
36	40261.5
37	44289.0
38	47061.5
39	49834.0
40	49834.0
41	53337.5
42	56841.0
43	59101.0
44	61361.0
45	62341.0
46	63321.0
47	63321.0
48	63777.5
49	64234.0
50	67908.5
51	71583.0
52	76902.5
53	82222.0
54	82222.0
55	82790.0
56	83358.0
57	77292.0
58	71226.0
59	62373.0
60	53520.0
61	53520.0
62	53914.0
63	54308.0
64	46685.0
65	39062.0
66	32574.5
67	26087.0
68	26087.0
69	23007.0
70	19927.0
71	19341.0
72	18755.0
73	23695.0
74	28635.0
75	28635.0
76	28225.0
77	27815.0
78	21292.0
79	14769.0
80	8784.5
81	2800.0
82	2800.0
83	1797.5
84	795.0
85	580.0
86	365.0
87	279.0
88	193.0
89	193.0
90	151.0
91	109.0
92	67.5
93	26.0
94	18.5
95	11.0
96	11.0
97	6.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1099936.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.31735377279306
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.57236239119824	30.06560316198868
2	13.194140483875149	10.63905659232651
3	4.732400368434077	5.723935795459587
4	2.208547857990622	3.5617122125900895
5	1.2216868595414283	2.4627590657852156
6	0.765422991372441	1.8515897717395338
7	0.5240854426099328	1.4790816737812846
8	0.3889120008307344	1.2543922179182005
9	0.2920144653542194	1.0595925455813175
>10	1.8011435694649396	13.718307373339695
>50	0.16211447246879862	4.493099915038453
>100	0.10542748771802075	9.01511213155331
>500	0.02153894906023804	6.130034921328016
>1k	0.009749208522002482	7.3396612632247
>5k	4.534515591629061E-4	1.2060613583454143
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	6614	0.6013077124487243	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6580	0.5982166235126407	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4896	0.4451168067960318	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	4745	0.43138873534460187	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4101	0.3728398743199604	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2953	0.26847016553690395	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2855	0.25956055625054547	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2780	0.25274197771506707	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	2443	0.22210383149565063	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	2330	0.21183050650219648	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2308	0.2098303901317895	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2196	0.19964797951880836	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	2168	0.1971023768655631	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	2140	0.19455677421231782	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2069	0.18810185319873157	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1997	0.18155601780467226	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	1957	0.17791944258575043	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	1902	0.17291915165973293	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1877	0.1706462921479068	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1844	0.16764611759229628	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1794	0.163100398568644	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1760	0.16000930963256044	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1721	0.15646364879411168	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1578	0.14346289238646612	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	1460	0.13273499549064674	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	1421	0.12918933465219795	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	1407	0.12791653332557532	No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC	1381	0.12555275943327612	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	1375	0.12500727315043783	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	1356	0.12327989992144998	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	1351	0.12282532801908475	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	1344	0.12218892735577343	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	1287	0.11700680766880982	No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA	1264	0.11491577691792977	No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA	1256	0.1141884618741454	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	1239	0.11264291740610362	No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC	1148	0.10436970878305647	No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG	1138	0.10346056497832601	No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC	1122	0.10200593489075728	No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG	1116	0.101460448607919	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	9.09143804730457E-5	0.0
13	0.0	0.0	0.0	9.09143804730457E-5	0.0
14	0.0	0.0	0.0	3.636575218921828E-4	0.0
15	0.0	0.0	0.0	5.454862828382743E-4	0.0
16	0.0	0.0	0.0	8.182294242574114E-4	0.0
17	0.0	0.0	0.0	0.0011818869461495943	0.0
18	0.0	0.0	0.0	0.0016364588485148228	0.0
19	0.0	0.0	0.0	0.002091030750880051	0.0
20	0.0	0.0	0.0	0.002818345794664417	0.0
21	0.0	0.0	0.0	0.003909318360340966	0.0
22	0.0	0.0	0.0	0.004909376545544468	0.0
23	0.0	0.0	0.0	0.006545835394059291	0.0
24	0.0	0.0	0.0	0.008818694905885433	0.0
25	0.0	0.0	0.0	0.010000581852035027	0.0
26	0.0	0.0	0.0	0.014728129636633404	0.0
27	0.0	0.0	0.0	0.032092776306985135	0.0
28	0.0	0.0	0.0	0.08409580193756727	0.0
29	0.0	0.0	0.0	0.16282765542722485	0.0
30	0.0	0.0	0.0	0.2714703400925145	0.0
31	0.0	0.0	0.0	0.598671195415006	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	25	0.005496242	29.6	1
TGCATCA	1460	0.0	19.767122	14
GGACAGG	1520	0.0	19.717106	6
ACAGGCT	1540	0.0	19.100649	8
CAGGACA	1560	0.0	19.092949	4
GCATCAG	1495	0.0	19.056856	15
GACAGGC	1510	0.0	18.990067	7
CTGCATC	1545	0.0	18.679611	13
CCAGGAC	1655	0.0	18.667675	3
GCTGCAT	1530	0.0	18.620914	12
AGCGTCA	70	1.2191364E-4	18.5	3
ATAGGCG	110	3.8511644E-8	18.5	32
ACCGAGT	50	0.007034695	18.5	8
TTATACG	70	1.2191364E-4	18.5	35
ACAATCG	50	0.007034695	18.5	37
CGATAGA	90	2.1520445E-6	18.5	37
TCCAGGA	1695	0.0	18.00885	2
GGCCATC	1655	0.0	17.661633	26
TGGACCG	75	2.0671637E-4	17.266666	5
TTAGAAC	65	0.0015798752	17.076923	3
>>END_MODULE