FastQCFastQC Report
Fri 10 Feb 2017
ERR1630352.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630352.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208278
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44802.1509712979767426No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41932.0131746992001074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27531.321791067707583No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16450.7898097734758351No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA7330.3519334735305697No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6170.2962386809936719No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA5810.2789540902063588No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.23046121049750812No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA4610.22133878758198175No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.18820998857296498No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3330.15988246478264626No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2810.13491583364541623No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2800.1344357061235464No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.12387290064241063No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2180.10466779976761828No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA2170.10418767224574846No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTAA200.001838477437.08
TCAAGAC200.001838477437.03
GGACCAG401.5917522E-632.37510
ACGGACC352.3789964E-531.7142878
CCAGGCC250.005486143829.5999984
CGGTAGG250.005486143829.59999829
AACAGAG250.005486143829.5999982
ATTTCCT250.005486143829.59999810
TACTGTG453.987572E-628.7777777
CGCCGGA453.987572E-628.77777714
GAAGGGA509.044506E-625.89999819
GACCAGA451.3181362E-424.66666611
ACCAGAG451.3181362E-424.66666612
CGCAAGA451.3181362E-424.6666662
CTGTGCG451.3181362E-424.6666669
TCTTATA1700.023.94117737
CGGAAGG551.8929773E-523.54545417
GCAAGAC551.8929773E-523.5454543
TCACATT551.8929773E-523.54545426
GCCGGAA551.8929773E-523.54545415