##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630352.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208278
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.67922680263878	31.0	31.0	34.0	30.0	34.0
2	31.839939888034262	33.0	31.0	34.0	30.0	34.0
3	31.888384754990927	33.0	31.0	34.0	30.0	34.0
4	35.591027376871295	37.0	35.0	37.0	33.0	37.0
5	35.56686255869559	37.0	35.0	37.0	33.0	37.0
6	35.65159546375517	37.0	35.0	37.0	33.0	37.0
7	35.623666445808006	37.0	35.0	37.0	33.0	37.0
8	35.679342033244026	37.0	35.0	37.0	33.0	37.0
9	37.31998578822535	39.0	37.0	39.0	34.0	39.0
10	37.21362793958075	39.0	37.0	39.0	34.0	39.0
11	37.32424932061956	39.0	37.0	39.0	34.0	39.0
12	37.25884154831523	39.0	37.0	39.0	34.0	39.0
13	37.28255504662038	39.0	37.0	39.0	34.0	39.0
14	38.346536840184754	40.0	38.0	41.0	34.0	41.0
15	38.34535572648095	40.0	38.0	41.0	34.0	41.0
16	38.23299628381298	40.0	38.0	41.0	33.0	41.0
17	38.34455871479465	40.0	38.0	41.0	34.0	41.0
18	38.36441678909919	40.0	38.0	41.0	34.0	41.0
19	38.42745273144547	40.0	38.0	41.0	34.0	41.0
20	38.4556026080527	40.0	38.0	41.0	34.0	41.0
21	38.40981284629198	40.0	38.0	41.0	34.0	41.0
22	38.345893469305445	40.0	38.0	41.0	34.0	41.0
23	38.275281114664054	40.0	38.0	41.0	34.0	41.0
24	38.212888543197074	40.0	38.0	41.0	33.0	41.0
25	38.182371637907025	40.0	37.0	41.0	33.0	41.0
26	37.92397660818713	40.0	37.0	41.0	33.0	41.0
27	37.71964393742978	40.0	37.0	41.0	33.0	41.0
28	37.610218073920436	40.0	36.0	41.0	33.0	41.0
29	37.47520141349543	39.0	36.0	41.0	32.0	41.0
30	37.26861214338528	39.0	36.0	41.0	32.0	41.0
31	37.068283736160325	39.0	35.0	41.0	32.0	41.0
32	36.82903139073738	39.0	35.0	41.0	31.0	41.0
33	36.63103640326871	39.0	35.0	41.0	31.0	41.0
34	36.50085942826415	39.0	35.0	40.0	31.0	41.0
35	36.285642266586	38.0	35.0	40.0	30.0	41.0
36	36.06179241206465	38.0	35.0	40.0	30.0	41.0
37	35.85186145440229	38.0	35.0	40.0	29.0	41.0
38	35.62540930871239	38.0	35.0	40.0	28.0	41.0
39	35.38580647019848	38.0	35.0	40.0	27.0	41.0
40	35.081160756296875	38.0	34.0	40.0	25.0	41.0
41	34.77108960139813	38.0	34.0	40.0	23.0	41.0
42	34.488664189208656	38.0	34.0	40.0	22.0	41.0
43	33.31104581376814	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	2.0
14	0.0
15	0.0
16	1.0
17	5.0
18	10.0
19	33.0
20	46.0
21	92.0
22	176.0
23	321.0
24	566.0
25	887.0
26	1379.0
27	1882.0
28	2679.0
29	3459.0
30	4555.0
31	5546.0
32	7161.0
33	8843.0
34	11352.0
35	14461.0
36	20238.0
37	32164.0
38	43871.0
39	48547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.243376640835805	20.75303200530061	13.320177839234102	20.683413514629486
2	18.297179730936534	25.146198830409354	33.32133014528659	23.235291293367517
3	21.29653636005723	27.09119542150395	31.080575000720188	20.531693217718626
4	13.994276879939314	17.667732549765216	34.194201980045904	34.14378859024957
5	13.038823111418393	40.404651475431876	32.09220368929988	14.464321723849855
6	29.89946129692046	35.36667338845197	17.56402500504134	17.169840309586228
7	25.141397555190657	34.24173460471101	22.119474932542083	18.497392907556247
8	29.636831542457674	32.21175544224546	20.147591200222777	18.003821815074083
9	27.833472570314676	12.753147235905857	22.261112551493678	37.15226764228579
10	20.058767608676863	29.297381384495726	28.85470380933176	21.789147197495655
11	32.14549784422743	25.4842086058057	19.669864315962318	22.700429234004552
12	19.838389076138622	30.53322962578861	28.749555882042273	20.878825416030498
13	30.751687648239372	20.963327859879584	26.626431980333976	21.658552511547065
14	22.524222433478332	23.31067131430108	28.500849825713708	25.66425642650688
15	27.146890214040848	27.274604134858215	24.96038947944574	20.618116171655192
16	21.42713104600582	26.79159584785719	28.617520813528074	23.163752292608915
17	20.213848798240814	28.910878729390525	28.542140792594513	22.333131679774148
18	19.09755230989351	25.87215164347651	31.493484669528225	23.536811377101756
19	19.840309586226102	27.42200328407225	33.50425873111899	19.233428398582664
20	20.998377168976077	25.630647499975996	33.280519305927655	20.09045602512027
21	22.15020309394175	26.21304218400407	32.10372674982475	19.533027972229426
22	22.392667492486005	26.47327130085751	31.183322290400326	19.950738916256157
23	20.741989072297603	27.16849595252499	31.575106348246095	20.514408626931314
24	19.97042414465282	28.1734028557985	31.73930996072557	20.11686303882311
25	20.309874302614777	27.919895524251242	31.486762884222045	20.283467288911936
26	20.517289392062533	28.43123133504259	31.357128453317202	19.69435081957768
27	20.181680254275534	28.34864940128098	31.768597739559628	19.70107260488386
28	19.36882435974995	29.55568999126168	31.77820028999702	19.29728535899135
29	19.54647154284178	28.87486916525029	31.730187537810046	19.848471754097886
30	18.750420111581636	30.751207520717504	32.086442159037446	18.41193020866342
31	19.795177599170337	29.458224104322106	31.449312937516204	19.29728535899135
32	18.55884923035558	30.10159498362765	31.227974149934223	20.111581636082544
33	18.893978240620708	30.055022614006283	31.647125476526565	19.403873668846447
34	18.71729131257262	30.058863634181236	31.534295508887162	19.689549544358982
35	19.276159748029077	30.326294663862726	31.094978826376284	19.302566761731914
36	18.80803541420601	30.466491900248705	30.842431749872766	19.883040935672515
37	18.981361449601014	30.663824311737198	30.693592218093123	19.661222020568665
38	18.74417845379733	30.045420063568884	31.11370379972921	20.09669768290458
39	18.76770470236895	29.5076772390747	32.02930698393494	19.69531107462142
40	18.480108316768934	29.93018945832013	31.92944045938601	19.660261765524922
41	18.41817186644773	29.355476814641968	32.07827999116565	20.14807132774465
42	17.46703924562364	30.325334408818982	32.05283323250656	20.154793113050825
43	18.001901304986603	29.916745887707773	31.3364829698768	20.74486983742882
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	50.0
1	69.5
2	89.0
3	275.5
4	462.0
5	462.0
6	659.0
7	856.0
8	850.0
9	844.0
10	1199.5
11	1555.0
12	1555.0
13	3070.5
14	4586.0
15	7006.5
16	9427.0
17	7815.0
18	6203.0
19	6203.0
20	7068.0
21	7933.0
22	5620.5
23	3308.0
24	3599.0
25	3890.0
26	3890.0
27	4189.0
28	4488.0
29	4590.0
30	4692.0
31	5075.0
32	5458.0
33	5458.0
34	6427.5
35	7397.0
36	7892.0
37	8387.0
38	9306.5
39	10226.0
40	10226.0
41	11015.0
42	11804.0
43	12614.0
44	13424.0
45	14171.5
46	14919.0
47	14919.0
48	16488.5
49	18058.0
50	16358.0
51	14658.0
52	13482.5
53	12307.0
54	12307.0
55	11553.0
56	10799.0
57	10096.5
58	9394.0
59	8421.5
60	7449.0
61	7449.0
62	6344.5
63	5240.0
64	4407.5
65	3575.0
66	3007.0
67	2439.0
68	2439.0
69	2077.0
70	1715.0
71	1403.5
72	1092.0
73	877.5
74	663.0
75	663.0
76	517.5
77	372.0
78	285.0
79	198.0
80	171.5
81	145.0
82	145.0
83	108.5
84	72.0
85	59.5
86	47.0
87	42.0
88	37.0
89	37.0
90	25.0
91	13.0
92	8.0
93	3.0
94	3.5
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	208278.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.792383256993055
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.45160651517402	28.105704875214858
2	11.868161790187633	9.20788561441919
3	4.925986434972028	5.7327226111255145
4	2.613990791623348	4.056117304756143
5	1.608990544086341	3.1208288921537557
6	1.024803208079608	2.3852735286492095
7	0.8453388781622853	2.295489682059555
8	0.6237932570919352	1.9358741681790685
9	0.5000247537006782	1.7457436695186241
>10	3.2303579385118075	23.838331460836
>50	0.230209416307738	6.025600399466098
>100	0.06807267686519135	4.347554710531117
>500	0.0037130551017377097	0.9271262447306006
>1k	0.00495074013565028	6.275746838360269
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4480	2.1509712979767426	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4193	2.0131746992001074	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2753	1.321791067707583	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1645	0.7898097734758351	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	733	0.3519334735305697	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	617	0.2962386809936719	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	581	0.2789540902063588	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	480	0.23046121049750812	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	461	0.22133878758198175	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	392	0.18820998857296498	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	333	0.15988246478264626	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	281	0.13491583364541623	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	280	0.1344357061235464	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	258	0.12387290064241063	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	218	0.10466779976761828	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	217	0.10418767224574846	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.801275218698086E-4	0.0
10	0.0	0.0	0.0	4.801275218698086E-4	0.0
11	0.0	0.0	0.0	9.602550437396172E-4	0.0
12	0.0	0.0	0.0	9.602550437396172E-4	0.0
13	0.0	0.0	0.0	9.602550437396172E-4	0.0
14	0.0	0.0	0.0	9.602550437396172E-4	0.0
15	0.0	0.0	0.0	0.0014403825656094259	0.0
16	0.0	0.0	0.0	0.0019205100874792344	0.0
17	0.0	0.0	0.0	0.002400637609349043	0.0
18	0.0	0.0	0.0	0.002400637609349043	0.0
19	0.0	0.0	0.0	0.0028807651312188517	0.0
20	0.0	0.0	0.0	0.0028807651312188517	0.0
21	0.0	0.0	0.0	0.0052814027405678945	0.0
22	0.0	0.0	0.0	0.0067217853061773206	0.0
23	0.0	0.0	0.0	0.012963443090484833	0.0
24	0.0	0.0	0.0	0.022085866006011198	0.0
25	0.0	0.0	0.0	0.02496663113723005	0.0
26	0.0	0.0	0.0	0.030248033877797943	0.0
27	0.0	0.0	0.0	0.051373644840069524	0.0
28	0.0	0.0	0.0	0.08210180623973727	0.0
29	0.0	0.0	0.0	0.12339277312054081	0.0
30	0.0	0.0	0.0	0.20837534449149694	0.0
31	0.0	0.0	0.0	0.4287538770297391	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAA	20	0.0018384774	37.0	8
TCAAGAC	20	0.0018384774	37.0	3
GGACCAG	40	1.5917522E-6	32.375	10
ACGGACC	35	2.3789964E-5	31.714287	8
CCAGGCC	25	0.0054861438	29.599998	4
CGGTAGG	25	0.0054861438	29.599998	29
AACAGAG	25	0.0054861438	29.599998	2
ATTTCCT	25	0.0054861438	29.599998	10
TACTGTG	45	3.987572E-6	28.777777	7
CGCCGGA	45	3.987572E-6	28.777777	14
GAAGGGA	50	9.044506E-6	25.899998	19
GACCAGA	45	1.3181362E-4	24.666666	11
ACCAGAG	45	1.3181362E-4	24.666666	12
CGCAAGA	45	1.3181362E-4	24.666666	2
CTGTGCG	45	1.3181362E-4	24.666666	9
TCTTATA	170	0.0	23.941177	37
CGGAAGG	55	1.8929773E-5	23.545454	17
GCAAGAC	55	1.8929773E-5	23.545454	3
TCACATT	55	1.8929773E-5	23.545454	26
GCCGGAA	55	1.8929773E-5	23.545454	15
>>END_MODULE