FastQCFastQC Report
Fri 10 Feb 2017
ERR1630351.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630351.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1199770
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79610.6635438459037982No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54140.4512531568550639No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50260.41891362511147967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23560.19637097110279472No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT13920.11602223759553915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG451.3232595E-424.6666665
CGCGAAC555.1432755E-420.18181828
ATACCGA759.266329E-619.7333346
CTTATAC8050.019.30434837
AAGACGG2950.018.8135595
CGTAAAT609.2371355E-418.515
CAATACT1054.7976937E-717.6190474
GCGTAAA650.001579988517.07692314
CGCAAGA3350.016.5671632
CCAATAC1251.6577724E-716.2799993
GTTATAC803.3827193E-416.18753
GCGCAAG3550.016.154931
CAAGACG3350.016.0149254
CTAGCGG2550.015.96078429
GTCCTAA1659.731593E-1015.696971
CGGACCA3300.015.696979
TCTAGCG2600.015.65384628
GACGGAC3800.015.0921067
TCTTATA14100.015.08865337
GTAGAAC1601.0963049E-815.0312513
Produced by FastQC (version 0.11.5)