##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630351.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1199770
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.705493552930978	31.0	31.0	34.0	30.0	34.0
2	31.920352234178218	33.0	31.0	34.0	30.0	34.0
3	32.0378189152921	33.0	31.0	34.0	30.0	34.0
4	35.7196479325204	37.0	35.0	37.0	33.0	37.0
5	35.64945031130967	37.0	35.0	37.0	33.0	37.0
6	35.70792901972878	37.0	35.0	37.0	33.0	37.0
7	35.68227410253632	37.0	35.0	37.0	33.0	37.0
8	35.69112746609767	37.0	35.0	37.0	33.0	37.0
9	37.41062787034181	39.0	37.0	39.0	34.0	39.0
10	37.295046550588864	39.0	37.0	39.0	34.0	39.0
11	37.36905240171033	39.0	37.0	39.0	34.0	39.0
12	37.28197487851838	39.0	37.0	39.0	34.0	39.0
13	37.29544162631171	39.0	37.0	39.0	34.0	39.0
14	38.4679630262467	40.0	38.0	41.0	34.0	41.0
15	38.46999008143227	40.0	38.0	41.0	34.0	41.0
16	38.41981879860307	40.0	38.0	41.0	34.0	41.0
17	38.41077539861807	40.0	38.0	41.0	34.0	41.0
18	38.36743875909549	40.0	38.0	41.0	34.0	41.0
19	38.39943989264609	40.0	38.0	41.0	34.0	41.0
20	38.39615592988656	40.0	38.0	41.0	34.0	41.0
21	38.3239704276653	40.0	38.0	41.0	34.0	41.0
22	38.27763154604632	40.0	38.0	41.0	34.0	41.0
23	38.20298723922085	40.0	38.0	41.0	33.0	41.0
24	38.14759578919293	40.0	38.0	41.0	33.0	41.0
25	38.12911558048626	40.0	38.0	41.0	33.0	41.0
26	37.948881869024895	40.0	37.0	41.0	33.0	41.0
27	37.817450844745245	40.0	37.0	41.0	33.0	41.0
28	37.716315627161876	40.0	37.0	41.0	32.0	41.0
29	37.640119356209944	40.0	37.0	41.0	32.0	41.0
30	37.54380756311626	40.0	37.0	41.0	32.0	41.0
31	37.46825891629229	40.0	37.0	41.0	32.0	41.0
32	37.351763254623805	39.0	36.0	41.0	32.0	41.0
33	37.23227618626903	39.0	36.0	41.0	31.0	41.0
34	37.17327571117798	39.0	36.0	41.0	31.0	41.0
35	37.00945847954191	39.0	36.0	41.0	31.0	41.0
36	36.90790818240163	39.0	35.0	41.0	31.0	41.0
37	36.83517757570201	39.0	35.0	41.0	30.0	41.0
38	36.69649016061412	39.0	35.0	41.0	30.0	41.0
39	36.60714303574852	39.0	35.0	41.0	30.0	41.0
40	36.465488385273844	39.0	35.0	40.0	30.0	41.0
41	36.32869299949157	39.0	35.0	40.0	30.0	41.0
42	36.25136401143553	39.0	35.0	40.0	30.0	41.0
43	35.00732807121365	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	2.0
12	0.0
13	4.0
14	4.0
15	7.0
16	15.0
17	40.0
18	81.0
19	173.0
20	274.0
21	521.0
22	916.0
23	1661.0
24	2536.0
25	3953.0
26	5900.0
27	8545.0
28	12093.0
29	16131.0
30	21220.0
31	27431.0
32	35099.0
33	45122.0
34	58690.0
35	77154.0
36	108288.0
37	163011.0
38	277837.0
39	333055.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.948456787550946	19.64468189736366	13.225201496953584	25.181659818131806
2	18.22924393842153	21.825933303883243	34.58079465230836	25.364028105386865
3	19.322370120939848	23.32272018803604	30.76648024204639	26.588429448977717
4	14.336664527367745	15.976145427873675	34.84192803620694	34.84526200855164
5	14.41667986364053	37.06535419288697	33.67228718837777	14.845678755094728
6	33.549430307475596	35.981896530168285	15.967143702542987	14.501529459813131
7	28.783266792801953	31.551130633371393	20.99810797069438	18.66749460313227
8	27.685889795544146	33.521841686323214	19.895730014919526	18.89653850321312
9	26.79030147444927	14.284071113630112	20.108604149128585	38.817023262792034
10	17.337739733448913	26.91224151295665	31.803095593322055	23.946923160272384
11	35.81978212490727	22.199921651649902	21.210398659743117	20.76989756369971
12	21.650566358552055	26.210356985088808	29.01006026155013	23.129016394809003
13	30.011002108737507	19.503654867182878	25.09839385882294	25.386949165256674
14	22.89522158413696	21.111129633179694	25.315352109154254	30.678296673529093
15	25.335856039074155	27.97511189644682	22.40504430015753	24.283987764321495
16	24.55829033898164	26.654858847945857	24.203972428048708	24.582878385023797
17	23.353309384298658	26.6155179742784	25.755019712111487	24.27615292931145
18	23.270210123607026	24.895771689573834	27.414587795994233	24.41943039082491
19	23.755469798378023	25.65216666527751	27.35799361544296	23.234369920901507
20	24.653308550805573	24.769080740475257	26.735374280070346	23.842236428648825
21	25.521391600056674	25.254757161789342	25.982396626019987	23.241454612133992
22	25.0162114405261	25.074889353792813	26.26361719329538	23.645282012385707
23	23.933420572276354	25.209665185827284	26.492077648215904	24.364836593680455
24	23.872742275602825	25.898630570859417	26.59934820840661	23.629278945131148
25	24.121123215282932	25.042549822049224	26.471907115530474	24.364419847137366
26	23.827233553097678	26.062745359527245	26.589096243446658	23.52092484392842
27	24.374171716245613	25.24142127241055	26.313543429157253	24.070863582186586
28	23.475582820040508	25.772273018995307	27.165790109771038	23.586354051193144
29	23.181693157855253	25.89838052293356	27.230969269109913	23.68895705010127
30	23.141935537644713	26.668778182484974	27.003008910041093	23.186277369829217
31	23.92491894279737	26.26595097393667	26.110421164056447	23.698708919209515
32	23.16702367953858	25.84378672578911	26.372804787584286	24.616384807088025
33	22.866216024738076	25.670503513173358	27.229052235011707	24.234228227076855
34	23.89616343132434	25.243088258582898	27.060269885061306	23.800478425031464
35	23.712211507205545	25.23233619777124	27.628378772598083	23.427073522425133
36	22.685848120889837	26.065912633254705	27.152204172466387	24.096035073389068
37	23.39323370312643	25.32377038932462	27.26547588287755	24.017520024671395
38	22.73052335030881	25.032964651558213	28.0746309709361	24.16188102719688
39	23.122931895279926	24.68339765121648	27.941772172999823	24.251898280503763
40	22.497978779266028	24.919026146678114	28.841694658142806	23.74130041591305
41	21.833851488201905	25.05180159530577	29.306450402993907	23.80789651349842
42	22.445885461380097	24.881685656417478	29.1700909340957	23.50233794810672
43	21.726580928011202	24.24573043166607	29.236186935829366	24.79150170449336
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	227.0
1	220.0
2	213.0
3	523.0
4	833.0
5	833.0
6	1160.5
7	1488.0
8	1405.0
9	1322.0
10	1851.5
11	2381.0
12	2381.0
13	4368.5
14	6356.0
15	11007.5
16	15659.0
17	13719.5
18	11780.0
19	11780.0
20	13143.0
21	14506.0
22	12838.0
23	11170.0
24	13106.5
25	15043.0
26	15043.0
27	17307.5
28	19572.0
29	23410.0
30	27248.0
31	30937.5
32	34627.0
33	34627.0
34	39194.5
35	43762.0
36	48861.5
37	53961.0
38	58769.5
39	63578.0
40	63578.0
41	67650.5
42	71723.0
43	72465.5
44	73208.0
45	76755.5
46	80303.0
47	80303.0
48	82546.5
49	84790.0
50	83099.5
51	81409.0
52	84063.5
53	86718.0
54	86718.0
55	83185.0
56	79652.0
57	76469.5
58	73287.0
59	68715.5
60	64144.0
61	64144.0
62	58989.0
63	53834.0
64	47214.5
65	40595.0
66	34862.5
67	29130.0
68	29130.0
69	24821.0
70	20512.0
71	17725.0
72	14938.0
73	11767.0
74	8596.0
75	8596.0
76	6705.5
77	4815.0
78	4078.5
79	3342.0
80	2725.5
81	2109.0
82	2109.0
83	1593.5
84	1078.0
85	885.0
86	692.0
87	673.0
88	654.0
89	654.0
90	534.5
91	415.0
92	239.5
93	64.0
94	43.0
95	22.0
96	22.0
97	17.0
98	12.0
99	7.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1199770.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.93905327728172
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.25944114159957	42.994996063286315
2	13.023399635011277	14.309864927984314
3	4.1209846420580245	6.792089844146567
4	1.6376413318884286	3.598818575267879
5	0.8130159891110347	2.2333164370526517
6	0.4572688462512935	1.5073150503744575
7	0.2770255156611941	1.0653663694853748
8	0.19848529945015927	0.8723675552999611
9	0.1382062696972105	0.6833629452734472
>10	0.8907334225799288	9.459819949639524
>50	0.09588326491442904	3.728534547075836
>100	0.08152638456601356	8.663235137457978
>500	0.0050193449519546785	1.8909482223292688
>1k	9.126081730826688E-4	0.6623207216484063
>5k	4.563040865413344E-4	1.5376436536780624
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7961	0.6635438459037982	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5414	0.4512531568550639	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5026	0.41891362511147967	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2356	0.19637097110279472	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	1392	0.11602223759553915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	8.334930861748502E-5	0.0
13	0.0	0.0	0.0	1.6669861723497004E-4	0.0
14	0.0	0.0	0.0	2.500479258524551E-4	0.0
15	0.0	0.0	0.0	3.333972344699401E-4	0.0
16	0.0	0.0	0.0	5.834451603223952E-4	0.0
17	0.0	0.0	0.0	7.501437775573652E-4	0.0
18	0.0	0.0	0.0	7.501437775573652E-4	0.0
19	0.0	0.0	0.0	0.0010835410120273053	0.0
20	0.0	0.0	0.0	0.0011668903206447903	0.0
21	0.0	0.0	0.0	0.0014169382464972453	0.0
22	0.0	0.0	0.0	0.0023337806412895806	0.0
23	0.0	0.0	0.0	0.003750718887786826	0.0
24	0.0	0.0	0.0	0.005501054368754011	0.0
25	0.0	0.0	0.0	0.006417896763546346	0.0
26	0.0	0.0	0.0	0.008418280170365987	0.0
27	0.0	0.0	0.0	0.02025388199404886	0.0
28	0.0	0.0	0.0	0.055260591613392564	0.0
29	0.0	0.0	0.0	0.11785592238512381	0.0
30	0.0	0.0	0.0	0.21045700425914968	0.0
31	0.0	0.0	0.0	0.5366028488793686	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	45	1.3232595E-4	24.666666	5
CGCGAAC	55	5.1432755E-4	20.181818	28
ATACCGA	75	9.266329E-6	19.733334	6
CTTATAC	805	0.0	19.304348	37
AAGACGG	295	0.0	18.813559	5
CGTAAAT	60	9.2371355E-4	18.5	15
CAATACT	105	4.7976937E-7	17.619047	4
GCGTAAA	65	0.0015799885	17.076923	14
CGCAAGA	335	0.0	16.567163	2
CCAATAC	125	1.6577724E-7	16.279999	3
GTTATAC	80	3.3827193E-4	16.1875	3
GCGCAAG	355	0.0	16.15493	1
CAAGACG	335	0.0	16.014925	4
CTAGCGG	255	0.0	15.960784	29
GTCCTAA	165	9.731593E-10	15.69697	1
CGGACCA	330	0.0	15.69697	9
TCTAGCG	260	0.0	15.653846	28
GACGGAC	380	0.0	15.092106	7
TCTTATA	1410	0.0	15.088653	37
GTAGAAC	160	1.0963049E-8	15.031251	3
>>END_MODULE